KAZAK 4 Flashcards
what is the structure of the mitochondrion?
double membrane
membrane invaginates to form cristae
makes the inter membrane space
matrix
how does NADH go through the ETC?
gives two electrons
makes one water
10 protons pumped in total
proton oxygen ratio is 10:1
how many electrons does each part of the ETC pump?
complex 1: 4
complex 3: 4
complex 4: 2
where are protons pumped to and from?
from the matrix into the inter membrane space
how does FADH go through the ETC?
does not go to complex 1
so only 6 protons pumped
first goes to complex 2 which is succinate dehydrogenase
what is the potential that is across the membrane?
membrane allows for ATP disequilibrium to occur
150-200 mV
what direction of reduction potential do electrons flow to?
from low to high reduction potential
what does complex 2 do?
ferries over electrons from complex 1 to complex 3
electrons from FADH go to carbon dioxide after it goes to complex 2 (succinate dehydrogenase)
what are the reduction potentials of complexes 1 and 4?
complex 1: -0.32, low, wants to give electrons away
complex 4:+0.82, high, good electron acceptor
what is the equation of NADH to make water?
NADH+H+1/2O2–> NAD++H2O
energy: -220kJ/mol
provides energy for ATP synthesis
how many subunits of the FO/C ring in mammals?
8 subunits
how many protons go through aTP synthase for every 360 turn and how many ATPs are produced?
8 protons go through
3 ATPs are produced
what is the P/O ratio through complex 1,3 and 4 and why is it that?
10/3.7=2.5
8/3=2.7 protons for every ATP molecule
you start with ADP so you need a Pi which is transported inside by symport
negative Pi is coupled to one proton, so therefore 2.7+1=3.7
what is the P/O ratio through the complex 2,3 and 4?
6/3.7=1.5
for FADH
