Key Concepts Flashcards

(60 cards)

1
Q

What is science?

A

-Science is both a body of knowledge and a process.
-It involves studying the natural world, which like biology focuses on living organisms.
-Science involves the following things:
1) Observation: Noticing something interesting or unusual. This leads us to our hypotheses, as the hypotheses are the proposed explanations for those observations.
2) Hypothesis Formation: Proposing an explanation based on imagination, intuition, logic, and previous knowledge.
3) Testing: Conducting experiments to evaluate the hypothesis.
4) Modification: Adjusting the hypothesis based on test results.

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2
Q

What characteristics does a scientific hypotheses require?

A

-A scientific hypotheses must be testable and falsifiable, meaning that it should have the possibility of being proven wrong through observation.
-Hypothesis testing is based on deductive reasoning: a general principle to predict an expected observation.
-The steps to creating a hypothesis are as follows:
1) Hypotheses that is testable and falsifiable
2) Make prediction
3) Test prediction

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3
Q

What are independent variables and dependent variables?

A

-Independent Variable: This is the variable that scientists manipulate or change to observe its effects. It is the factor that can be freely altered during the experiment. For example, if researchers are studying the effect of Vitamin C on cold prevention, the amount of vitamin C intake would be the independent variable.
-Dependent Variable: This variable is what scientists measure in the experiment. It is the outcome that may be affected by changes in the independent variable. In the vitamin C study, the dependent variable would be the indvidiuals’ susceptibility to illness upon exposure to a cold virus.

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4
Q

What are controlled experiments in hypothesis testing?

A

-Controlled experiments involve comparing an experimental group with a control group.
-The goal is to test hypotheses by ensuring that the only difference between the groups is the experimental treatment.
-This helps eliminate alternative hypotheses and isolate the effect of the treatment.

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5
Q

What two things are needed for controlled experiments?

A

1) Random Assignment: Subjects are randomly assigned to either the control group or the experimental group. This randomization helps ensure that the groups are as similar as possible, minimizing differences that could affect the results. For example, in a study on echinacea tea, participants were randomly assigned to groups to ensure a balanced representation of the population.

2) Identical Participation: Both groups should participate under identical conditions, except for the experimental treatment. This means that all participants receive the same information and undergo the same procedures, with the only difference being the treatment itself. In the echinacea study, both groups drank tea, but only the experimental group received tea with echinacea extract, while the control group received a placebo or “sham tea.”

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6
Q

What is the gold standard of hypotheses testing?

A

-Double-blind, placebo-controlled and randomized experiments are the gold standard.
-Double blind means that neither the participants nor the researchers know who is receiving the treatment or the placebo. This helps prevent bias from influencing the results.
-Placebo-controlled means that a placebo is used to ensure that any effects observed are due to the treatment itself and not participants expectations.
-Randomized means that participants are randomly assigned to either the treatment or control group, ensuring that the groups are comparable.

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7
Q

What are model systems?

A

-Model systems are alternative organisms used to test hypoetheses that would be impractical or unethical to test on humans.
-This involves testing on bacteria, nematodes, mammals(rodents, dogs, pigs, human cells).

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8
Q

What do statistical tests tell us in science?

A

-Statistical tests help to evaluate the likelihood that observed differences between experimental and control groups are not random.
-Statistical tests do carry the limitation that statistical significance does not equate to practical significance. A finding may be statistically significant, but not have meaningful applications in real world context.
-Applications may be limited. Just because a result is statistically significant, doesn’t mean it will change medical advice.

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9
Q

What are the eight key characteristics of life?

A

1) Common Set of Biological Molecules: All living organisms contain essential biological molecules, such as proteins, lipids, carbohydrates, and nucleic acids, which are crucial for various cellular functions.
2) Composed of Cells: The cell is the basic structural and funcitonal unit of life.
3) Growth: Living organisms exercising their ability to grow often involves an increase in size and number of cells.
4) Movement: While not all organisms move in the same way, movement is a common characteristic.
5) Reproduction: The ability to reproduce and pass genetic information.
6) Responses to External Environmental Stimuli: Organisms can respond to changes in their environment, which is crucial for survival.
7) Metabolism: This encompasses all chemical processes within cells, including energy production and waste excretion.
8) Maintain Homeostasis: Organisms regulate their internal environment to maintain stable conditions necessary for life, despite external changes.

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10
Q

What is a molecule?

A

A molecule is two or more atoms held together by chemical bonds.

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11
Q

What are macromolecules?

A

-Macromolecules are large organic molecules composed of smaller subunits. In living organisms, the primary macromolecules include:
1) Carbohydrates
2) Proteins
3) Lipids
4) Nucleic acids.

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12
Q

What are carbohydrates?

A

-Carbohydrates are essential for energy storage and structural functions.
-Carbohydrates are made of molecules of carbon, oxygen, and hydrogen.
-They can be simple sugars like monosaccharides or complex structures like polysaccharides, which consist of sugar monomers linked by covalent bonds.
-Monosaccharide means 1 sugar, while disaccharides means 2 sugars. Polysaccharides means many sugars.

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13
Q

What are proteins?

A

-Proteins perform many important functions in cells. They catalyze reaction, and serve as transport channels or chemical messengers.
-Proteins are made of carbon, oxygen, hydrogen, and nitrogen.
-They are made up of amino acids.
-There are 20 standard amino acids that can be used as building blocks to make all proteins in living organisms.
-Amino acids are joined by peptide bonds.

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14
Q

What are lipids?

A

-Lipids are crucial for storing energy and forming cell membranes.
-Lipids are composed mostly of carbon and hydrogen.
-They are typically composed of fatty acids and glycerol.

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15
Q

What are nucleic acids?

A

-Nucleic acids include DNA and RNA, which are vital for storing and transmitting genetic information.

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16
Q

What are cells? What are the different types of cells? How do plant and animal cells differ?

A

-Cells are the smallest, most basic unit of life.
-Cells are microscopic, self-contained units enclosed by a water-repelling membrane.
-The human body has 100 trillion cells.
-There are prokaryotic cells and eukaryotic cells.
-Prokaryotic cells lack a nucleus and are membrane-bound organelles. They are typically smaller and simpler, with ribosomes, a cell wall, a cell membrane, and a nucleoid region. Bacteria are common examples of prokaryotic organisms.
-Eukaryotic cells have a nucleus, the cell membrane, and various membrane-bound organelles, such as mitochondria and the endoplasmic reticulum. Eukaryotic cells are fund in plants, animals, fungi, and protists.
-Plant cells have a cell wall, chloroplasts, and large central vacuole.
-Animal cells lack a cell wall, chloroplasts, and have small multiple vacuoles. Instead, they have centrioles, which are involved in cell division?

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17
Q

Compare and contrast prokaryotes and eukaryotes?

A

1) Prokaryotes are small and microscopic, while eukaryotes are larger, and often single-celled or multicellular.
2) Prokaryotes are simple, while eukaryotes are complex.
3) Prokaryotes have genetic material, but not nucleus, while eukaryotes hold their genetic material inside their nucleus.
4) Prokaryotes don’t have organelles, while eukaryotes have membrane-bound organelles.
5) All prokaryotes have cell walls, while only some eukaryotes have cell walls.

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18
Q

Are viruses alive?

A

-Answering the question is difficult.
-Variuses exist in a gray area between the living and nonliving.
-Viruses must replicate within a host cell.
-Viruses are active agents of evoluation and often exchange genetic information with their hosts.
-Viruses may add their genes to the genome of their hosts, possibly becoming a critical part of the host.
-Viruses are not living due to their lack of cellular structure, dependence on host cells, absence of metabolic processes, and their structure(a virus typically consists of a strand of DNA or RNA encased in a protein shell, known as a capsid).

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19
Q

What is metabolism?

A

-Metabolism encompasses all the chemical processes that occur within cells. These processes include the following:
1) Energy Production: Metabolism involves breaking down substances to produce energy. This energy is crucial for various cellular activities and overall bodily functions.
2) Synthesis of Necessary Substances: It also includes the synthesis of compounds needed for life, such as proteins and nucleic acids.
3) Waste Excretion: Metabolic processes generate waste products, which must be excreted to maintaining cellular health.

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20
Q

What are enzymes?

A

-Enzymes are proteins that play a crucial role in metabolism by catalyzing, or speeding up chemical reactions in cells. Their key functions are as follows:
1) Catalysis: Enzymes lower the activation energy required for reactions, allowing them to occur more easily and without the need for excessive heat, which may damage cells.
2) Specificity: Each enzyme is specific to a particular reaction, determined by the shape of its active site and the substrate it binds to. This specificity ensrues that enzymes only catalyze the intended reactions.
3) Function: Enzymes can break down substances, such as food molecules, to release energy, or they can build complex molecules, to release energy, or they can build complex molecules from simpler ones.
4) Reusability: After catalyzing a reaction, enzymes are not permanently altered and can be used repeatedly.

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21
Q

What are the three key components of cells?

A

1) Cell Structure
2) Energy for Cells to Work
3) Cancer: cell growth out of control

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22
Q

What does the cell structure consist of?

A

-The cell structure includes the cytoplasms, cytosol, and organelles.
-Cytoplasm: A crucial component of the cell, encompassing both the cytosol and organelles. It acts as the medium where cellular processes occur.
-Cytosol: This is the watery matrix within the cytoplasm. It contains salts and enzymes necessary for cellular reactions and serves as the environment necessary for cellular reactions and serves as the environment.
-Organelles: These are specialized structures within the cell, each performing specific functions similar to how organs function in the body.
-There is the Nucleus that houses DNA. There is the mitochondria that is involved in energy production. Ribosomes are sites for protein synthesis. The Golgi Apparatus modifies and sorts proteins. Lysosomes are responsible for breaking down macromolecules.

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23
Q

What are the subcellular structures?

A

1) Cell Wall
2) Nucleus
3) Nucleolus
4) Mitochondrion
5) Chloroplast
6) Lysosome
7) Ribosomes
8) Rough endoplasmic reticulum
9) Smooth endoplasmic reticulum (smooth ER)
10) Golgi apparatus
11) Centrioles
12) Cytoskeletal elements
13) Central vacuole

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24
Q

What does the cell wall do?

A

-The cell wall is found in plant cells, fungi, and some bacteria.
-It is located outside the plasma membrane.
-It provides protection and structural support.
-The cell wall is composed primarily of cellulose, a polysaccharide that forms strong fibers.

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25
What does the nucleus do?
-The nucleus is present in all eukaryotic cells. -It is a spherical structure that is surrounded by a double membrane called the nuclear envelope. -The nucleus contains nuclear pores for regulating traffic in and out. -It houses chromatin, which is made up of DNA and proteins.
26
What does the mitochondrion do?
The mitochondrion produces energy for the cell through cellular respiration.
27
What does the chloroplast do?
The chloroplast produces sugars through photosynthesis in plant cells.
28
What does the lysosome do?
The lysosome contains digestive enzymes to recycle molecules.
29
What do ribosomes do?
-RIbosomes are essential cellular structures where proteins are assembled. -They act as workbenches for protein synthesis, translating genetic information from mRNA to build proteins. -Ribosomes are composed of two subunits, one large and one small, made of ribosomal RNA (rRNA) and proteins. -These subunits come together during protein synthesis to facilitate the translation process. -Ribosomes can be located free-floating in the cytoplasm, or attached to the endoplasmic reticulum.
30
What is the rough endoplasmic reticulum?
The rough endoplasmic reticulum is a membrane network with ribosomes attached for protein synthesis.
31
What is the smooth endoplasmic reticulum?
The smooth endoplasmic reticulum is involved in lipid synthesis or detoxificiation, and it lacks ribosomes.
32
What is the golgi apparatus?
The Golgi Apparatus modifies, sorts, and packages proteins.
33
What do centrioles do?
Centrioles move chromosomes during cell division.
34
What do cytoskeletal elements do?
Cytoskeletal elements form cytoskeleton for maintaining shape and structural support.
35
What does the central vacuole do?
The central vacuole stores water, sugars, and pigments in plant cells.
36
What substances travel through the plasma membrane?
-Substances like food and supplements must cross the plasma membrane to enter cells. This is crucial for synthesizing cell components and providing energy. -The plasma membrane is a phospholipid bilayer with a hydrophobic interior, making it selectively permeable. This means it allows some substances to pass while blocking others. -Molecules move from high to low concentration until equilibrium is reached, meaning equal concentrations on both sides of the membrane. This process is passive and doesn't require energy.
37
How do cancer cells differ from normal cells?
-Cancer cells differ from normal cells because they replicate when they should not, they invade nearby tissues, and they can move to other parts of the body. -These behaviors allow cancer to grow uncontrollably and spread throughout the body.
38
What is the cell cycle? What are the corresponding three stages?
The cell cycle is the series of stages that a cell goes through to grow and divide: and it consists of three phases: 1) Interphase: This is the longest phase where the cell prepares for division. 2) Mitosis: This is a type of cell division that results in two genetically identical daughter cells (2n), used for growth and repair in somatic cells. 3) Cytokinesis: The cytoplasm divides, resulting in two genetically identical daughter cells.
39
What is meiosis? What are gametes?
-Meiosis is a specialized cell division in gonads that reduces chromosome number by half, producing four genetically unique gametes (n). -Gametes are reproductive cells (sperm or egg) that are haploid (n), and they combine during fertilization to form a diploid (2n) zygote.
40
What are tumor suppresor genes?
Tumor suppressor genes are genes that encode proteins which stop cell division if DNA is damaged.
41
What are proto-oncogenes and oncogenes?
-Proto-oncogenes promote normal cell growth. When mutated, they become oncogenes and cause uncontrolled division.
42
What is sampling error?
Sampling error is a concept in statistics that refers to the difference between a sample and the population from which it is drawn.
43
What are the two types of nucleic acids?
1) DNA (Deocyribonucleic Acid): DNA is a double-stranded helix composed of nucleotides. DNA serves as the primary storage of genetic information in almost all living organisms. It encodes the isntructinos for building proteins, which are essential for various cellular functions. 2) RNA (Ribonucleic Acid): RNA is typically single-stranded and also made up of nucleotides. The sugar in RNA is ribose. RNA plays a critical role in protein synthesis, acting as a messenger in order to carry the genetic instructions from DNA to the ribosome, where proteins are synthesized.
44
What are the three key types of lipids?
1) Fats (Triglycerides): The structure is 1 glycerol and 3 fatty acid chains. The function is to help with long term energy storage, insulation, and protection. 2) Steroids (e.g. Cholesterol): The structure is 4 fused carbon rings. Cholesterol helps to stabilize animal cell membranes. 3) Phospholipids: The structure is 1 glycerol, 2 fatty acid tails, and 1 phosphate group. The phosphate head is hydrophilic (water loving), and the fatty acid tails is hydrophobic (water fearing). The function is to form cell membranes--the famous phospholipid bilayer.
45
What are lipids?
-Lipids are crucial for storing energy and forming cell membranes. -Lipids are composed mostly of carbon and hydrogen. -They are typically composed of fatty acids and glycerol.
46
What is organic chemistry?
-Organic chemistry is the branch of chemistry that focuses on carbon-containing molecules. -These molecules include carbohydrates, proteins, lipids, and nucleic acids. -Carbon's ability to interact with other elements through chemical bonds such as covalent bonds is crucial for forming the diverse structures found in biological systems.
47
What does the plasma membrane consist of?
-The Plasma Membrane has a phospholipid bilayer with a structure such that it is made up of phospholipids, each with a hydrophilic (water-attracting) head which maximizes exposure to water, and it has two hydrophobic (water-repelling) tails that excludes water. -It helps to form a flexible, self-sealing barrier that is semipermeable, allowing selective passage of substances. -Proteins are embedded in the phospholipid bilayer, and their role is to perform enzymatic functions, serve as receptors for outside substances, and help transport substances throughout the cell. -Sugar Chains extend from the outer surface of the plasma membrane, and are involved in cell recognition and communication.
48
What are the properties of water?
1) Polarity: Water molecules are polar, meaning they have regions with different charges. The oxygen atom carries a partial negative charge (δ−), while the hydrogen atoms have a partial positive charge (δ+). This polarity allows water molecules to form hydrogen bonds with each other, contributing to its unique characteristics. 2) Solvent Abilities: Water is an excellent solvent due to its polarity. It can dissolve a wide variety of substances, such as salts and polar molecules, by surrounding and separating their charged components. This ability is crucial for facilitating chemical reactions in biological systems. 3) Temperature Moderation: Water can absorb and retain heat, helping to moderate temperature changes in organisms and environments. This property is vital for maintaining stable conditions necessary for life.
49
What can water dissolve, and what can it not dissolve?
-Water can dissolve polar molecules, and hydrophilic substances. -Polar Molecules: Water can dissolve substances like sodium chloride (table salt) and alcohol. This is because the positive and negative poles of water molecules can attract and separate the charged components of these substances. -Hydrophilic Substances: These are substances that "love" water and mix well with it, typically because they contain charged atoms or polar bonds. -Water cannot dissolve Nonpolar Molecules: Substances like oil do not mix well with water. They are hydrophobic, meaning they "fear" water, as they lack charged atoms and do not interact with water's polar molecules.
50
What does the oxygen atom consist of?
-The oxygen atom has 8 positively charged protons located in the nucleus. -It has 8 neutrally charged neutrons also located in the nucleus. -It has 8 electrons that are negatively charged, orbiting in the electron cloud. -The oxygen atom is structured such that there are 2 electrons in the inner shell, and 6 in the outer shell. -Oyxgen is electronegative, meaning that it strongly pulls electrons, which is why it's vital in water (H2) and respiration.
51
What does the carbon atom consist of?
-The carbon consists of 6 protons, 6 neutrons, and 6 electrons. -Carbon has 2 electrons in the inner shell, and 4 in the outer shell. -Carbon can form four bonds, and it can form a combination of single and double bonds. -Carbon can form methane (CH4), and carbon dioxide (CO2). IT can also form glucose (C6H12O6).
52
What does the hydrogen atom consist of?
-The hydrogen atom has 1 proton, 0 neutrons, and 1 electron. -Hydrogen is very reactive and forms bonds easily, especially in water and organic compounds.
53
What genetic material is inside a virus?
-Viruses are unique entities that contain genetic material essential for their replication and infection processes. Here's a breakdown of the genetic material found in viruses: Types of Genetic Material: 1) DNA or RNA: A virus can have either DNA or RNA as its genetic material. This can be single-stranded or double-stranded, and it can be linear or circular. Examples: The herpes virus has a double-stranded DNA genome, while the coronavirus responsible for COVID-19 has a single-stranded RNA genome. Function of Viral Genes: 2) The genes in a virus code for proteins necessary to produce more viruses within a host cell. For instance, RNA viruses known as retroviruses carry the enzyme reverse transcriptase, which synthesizes DNA from their RNA genome. Viral Structure: 3) The genetic material is enclosed within a protein shell called a capsid. Some viruses also have an outer envelope derived from the host cell's membrane.
54
What are the four different nitrogen bases?
-DNA is composed of four nitrogenous bases that form the rungs of its double helix structure. These bases are: 1) Adenine (A): Adenine has a structure consisting of a pentagonal and hexagonal ring sharing a side. It pairs with Thymine (T) through two hydrogen bonds. 2) Thymine (T): Thymine is a hexagonal ring and pairs with Adenine (A) through two hydrogen bonds. 3) Guanine (G): Guanine also has a pentagonal and hexagonal ring sharing a side. It pairs with Cytosine (C) through three hydrogen bonds. 4) Cytosine (C): Cytosine is a hexagonal ring and pairs with Guanine (G) through three hydrogen bonds.
55
What are purines and pyrimidnes? How do purines form hydrogen bonds with pyrimidines?
-In DNA, purines and pyrimidines form hydrogen bonds to create the double helix structure. Here's how they pair: 1) Purines: These are larger, double-ring structures. Adenine (A) and Guanine (G) are purines. 2) Pyrimidines: These are smaller, single-ring structures. Thymine (T) and Cytosine (C) are pyrimidines. -Hydrogen bonding occurs when Adenine pairs with Thymine through two hydrogen bonds. This pairing is specific and consistent, ensuring the stability of the DNA structure. -Next, Guanine pairs with Cytosine through three hydrogen bonds. This bond is slightly stronger due to the additional hydrogen bond.
56
How do cells get their energy?
-Cells obtain energy primarily through a process called cellular respiration, which occurs in the mitochondria of both plant and animal cells. Here's a simplified breakdown of how this process works: 1) Glucose Breakdown: The process begins with glucose, a sugar molecule, which is broken down in a series of reactions. This breakdown releases energy. 2) Role of Oxygen: Oxygen is crucial in this process. It combines with hydrogen atoms from glucose to form water, while carbon and oxygen are released as carbon dioxide. 3) ATP Production: The energy released from glucose is used to synthesize ATP (adenosine triphosphate), the energy currency of the cell. ATP is generated by adding a phosphate group to ADP (adenosine diphosphate). 4) Metabolism of Other Nutrients: Besides glucose, cells can also metabolize proteins and fats to produce ATP, especially when carbohydrates are scarce. -This entire process is known as aerobic respiration because it requires oxygen. It efficiently converts the energy stored in food into a form that cells can use for various functions.
57
Describe the process of making ATP?
1) Cellular Respiration: This process occurs in the mitochondria and involves breaking down glucose in the presence of oxygen. The overall reaction converts glucose and oxygen into carbon dioxide, water, and ATP. 2) Phosphorylation: ATP is synthesized by adding a phosphate group to ADP (adenosine diphosphate). This process is powered by the energy released from glucose breakdown. 3) ATP's Role: Once formed, ATP can transfer its phosphate group to other molecules, a process known as phosphorylation. This transfer energizes the recipient molecule, enabling it to perform work, such as mechanical, transport, or chemical tasks within the cell. 4) Regeneration: Cells continuously use ATP, so it must be regenerated. This regeneration involves reattaching a phosphate group to ADP during cellular respiration.
58
What is the process of aerobic cellular respiration? How does oxygen get into the cell?
-Glucose+Oxygen gives us water+carbon dioxide. -C6H12O6+6O2 leads to 6H2O+6CO2. -Oxygen gets into the cell by diffusing across the plasma membrane (simple diffusion, since O2 is small and nonpolar).
59
How could we test for a cancer susceptibility gene?
-DNA profiling is a technique used to identify individuals based on differences in their DNA sequences. -Process: Instead of analyzing the entire genome, scientists focus on 13 specific DNA sequences known as short tandem repeats (STRs). -STRs: These are short sequences of DNA (four or five bases long) repeated in tandem and located between gene-coding regions on chromosomes. STRs are the 13 DNA sequences carried by all humans. Sientists know percentages of variation for specific STRs in the population. -DNA Profiling and PCR: DNA profiling often requires more DNA than what is easily obtainable from a suspect or crime scene. Small DNA segments are amplified using the polymerase chain reaction (PCR). -PCR Process: PCR involves heating and cooling cycles in an automated machine. Heating denatures DNA, breaking hydrogen bonds and resulting in single strands. Taq polymerase, a heat-tolerant enzyme, synthesizes new DNA strands using nucleotides and primers.
60
What is a phenotype? What are factors that alter phenotypical ratios?
-Phenotype refers to the observable physical traits of an organism, such as eye color, height, or seed shape in plants. These traits are influenced by the organism's genotype, which is the set of alleles it possesses, and by environmental factors. -The key facotrs affecting phenotypical ratios are as follows: 1) Genotype Composition: Homozygous: An individual with two identical alleles for a gene. Heterozygous: An individual with two different alleles for a gene. 2) Dominant and Recessive Alleles: Dominant Alleles: Expressed even if only one copy is present. For example, the allele for round seeds in pea plants is dominant. Recessive Alleles: Expressed only when two copies are present, such as the allele for wrinkled seeds. Environmental Influences: 3)Environmental conditions can also affect how genes are expressed, altering phenotypical outcomes.