Kligler Iron Agar (KIA) Flashcards

1
Q

What is the abbreviated reaction for this KIA result?

A

A/A (Acid/Acid)

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2
Q

What is the abbreviated reaction for this KIA result?

A

K/A (Alkaline/Acid)

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3
Q

What is the abbreviated reaction for this KIA result?

A

K/A, H2S

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4
Q

What is the abbreviated reaction for this KIA result?

A

K/NC (Alkaline/No Change)

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5
Q

TRUE or FALSE: This KIA result means you’re dealing with a lactose fermenter.

A

TRUE

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6
Q

TRUE or FALSE:
This KIA result means you’re dealing with Non-fermenters.

A

FALSE
This result is for Non-LACTOSE fermenters

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7
Q

For KIA, what would be the interpretation?

Fermenter, gas, abbreviation?

A

Non-lactose fermenter, H2S positive.
K/A, H2S

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8
Q

For KIA, what would be the interpretation?

Fermenter, gas, abbreviation

A

Nonfermenter, no gas production, K/NC

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9
Q

What organisms will produce a KIA result of A/A (yellow/yellow)?

A

Escherchia coli
Klebsiella
Enterobacter

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10
Q

What organisms will produce a KIA result of K/A (red/yellow)?

A

Shigella
Providencia
Morganella
Yersinia
Serratia
Plesiomonas shigelloides

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11
Q

What organisms will produce a KIA result of K/A, H2S?

A

Salmonella
Proteus

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12
Q

What organisms will produce a KIA result of K/NC?

A

Pseudomonas aeruginosa
Acinetobacter

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13
Q

What does the KIA media contain for reactions?

What is fermented/metabolized?

A

Carbohydrates: Glucose (1x) & Lactose (10x)
Peptones: protein fragments

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14
Q

What is the pH indicator used in KIA tubes?

A

Phenol Red

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15
Q

When sugars are metabolized, ____ are produced.

A

Acids

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16
Q

When amino acids/proteins (peptones) are metabolized, ________ products are formed.

A

Alkaline

17
Q

Which enterics are lactose fermenters and will turn KIA yellow/yellow?

A

Escherichia coli
Klebsiella
Enterobacter

18
Q

Which enterics are non-lactose fermenters and will turn KIA red/yellow?

A

Shigella
Providencia
Morganella
Yersinia
Serratia
Plesiomonas shigelloides

19
Q

Which enterics are non-lactose fermenters and produce H2S? KIA: K/A, H2S

A

Salmonella
Proteus

20
Q

Which enterics are nonfermenters?
KIA: K/NC

A

Pseudomonas aeruginosa
Acinetobacter