L18- Oxidative phosphorylation continued Flashcards Preview

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Flashcards in L18- Oxidative phosphorylation continued Deck (12):
1

What are the five complexes involved in?

Complexes I-IV are involved in electron transport
Complex V is an ATP synthase

2

In complex IV what's the source of electrons?

Cytochrome C

3

What is the net transfer in complex IV?

O2 + 4e- +4H+ for every pair of electrons. Makes H2O

4

What does the formation of H2O remove from the matrix?

2H+. 2 protons go to make water

5

What does complex V do?

It's the ATP synthase.
Uses the proton gradient to make ATP.
Knob and stalk structure.
F1= knob- contains the catalytic subunits
F0=stalk- has a proton channel

6

How are ATP and ADP transported across the inner mitochondrial membrane?

Adenosine nucleotide translocase. Antiport exchange of ATP for ADP

7

How are Pi and H+ transported across the inner mitochondrial membrane?

Symport of Pi and H+ together. It's electroneutral

8

What is the P:O ratio?

molecules of ADP phosphorylated / atoms of oxygen reduced

9

How many H+ have to be translocated by ATP synthase for each ATP produced?

3H+

10

How many H+ are needed to transport Pi, ADP and ATP?

1H+. It goes through with Pi as a symport

11

What's the net H+ needed to be transported for each ATP molecule?

4 H+.
3 for the atp synthase and 1 through the symport so that adp and atp can move across i think

12

Since 4H+ are required for ATP synthesis, what is the P:O ratio for NADH and succinate?

NADH- 10K+/ o (1 oxygen?)
10/4= 2.5 atp/o

succinate- 6H+/4H+ = 1.5 ATP/o