L18- Oxidative phosphorylation continued

Question 1

What are the five complexes involved in?

Answer 1

Complexes I-IV are involved in electron transport

Complex V is an ATP synthase

Question 2

In complex IV what’s the source of electrons?

Answer 2

Cytochrome C

Question 3

What is the net transfer in complex IV?

Answer 3

O2 + 4e- +4H+ for every pair of electrons. Makes H2O

Question 4

What does the formation of H2O remove from the matrix?

Answer 4

2H+. 2 protons go to make water

Question 5

What does complex V do?

Answer 5

It's the ATP synthase.
Uses the proton gradient to make ATP.
Knob and stalk structure. 
F1= knob- contains the catalytic subunits
F0=stalk- has a proton channel

Question 6

How are ATP and ADP transported across the inner mitochondrial membrane?

Answer 6

Adenosine nucleotide translocase. Antiport exchange of ATP for ADP

Question 7

How are Pi and H+ transported across the inner mitochondrial membrane?

Answer 7

Symport of Pi and H+ together. It’s electroneutral

Question 8

What is the P:O ratio?

Answer 8

molecules of ADP phosphorylated / atoms of oxygen reduced

Question 9

How many H+ have to be translocated by ATP synthase for each ATP produced?

Answer 9

3H+

Question 10

How many H+ are needed to transport Pi, ADP and ATP?

Answer 10

1H+. It goes through with Pi as a symport

Question 11

What’s the net H+ needed to be transported for each ATP molecule?

Answer 11

4 H+.

3 for the atp synthase and 1 through the symport so that adp and atp can move across i think

Question 12

Since 4H+ are required for ATP synthesis, what is the P:O ratio for NADH and succinate?

Answer 12

NADH- 10K+/ o (1 oxygen?)
10/4= 2.5 atp/o

succinate- 6H+/4H+ = 1.5 ATP/o