L22 Chlamydia

Question 1

Chlamydia
Gram
Special

Answer 1

Negative

No peptidoglycan

Question 2

Chlamydia growth

Answer 2

Obligate intracellular

Question 3

Chlamydia species

Answer 3

Trachomatis (human)
Pneumoniae (human)
psittaci
pecorum

Question 4

Chlamydia is leading cause of

Answer 4

blindness

Question 5

Chlamydia infectious dose

Answer 5

Small

Question 6

Chlamydia infects what type of cells

Answer 6

mucosal epithelial

Question 7

Chlamydia spread by

Answer 7

Fingers
Flies
Fomites
Fornication

Question 8

C. trachomatis symptoms

Answer 8

Male: prostitis, epididymitis
Women: usually asymptomatic
cervicitis, newborn eye infetion

Question 9

C. trachomatis infant infection leads to

Answer 9

conjunctivitis

pneumonia

Question 10

Chlamydia development

Answer 10

Elementary body enters epithelial cells

Turns to reticulate bodies which multiple

Question 11

How do EB get into cells

Answer 11

receptor mediated endocytosis

Question 12

How do EB modify the vesicle

Answer 12

maintain pH above 6.2
Prevent fusion with lysosome

Also adds glycolipids for camouflage

Question 13

RB divide by

Answer 13

binary fission

Question 14

Chlamydia development

Answer 14

Slow (2-3 days per cycle)

Question 15

RB nutrientt uptake

Answer 15

drinking staws that allow nutrient uptake without exiting inclusion vacuole

Question 16

Lymphogranuloma venerum significance

Answer 16

systemic, spreads to other areas

Question 17

Chlamydia pneumoniae can be observed in 40-100% of patients with

Answer 17

atherosclerotic heart lesions

Question 18

Layers to penetrate for chlamydia treatment

Answer 18

plasma membrane
inclusion membrane
chlamydial outer membrane
chlamydial cytoplasmic membrane

Question 19

Rickettsiae
Gram
special

Answer 19

Gram -

obligate intracellular bacteria

Question 20

Rickettsiae vs chlamydia

Answer 20

Rickettsiae capable of independent metabolism but just need host for replication

Question 21

Rickettsiae transmission

Answer 21

tick bite

Question 22

Rickettsiae ticks

Answer 22

Rocky mountain wood tick

American dog tick

Question 23

Rickettsiae attach to ____ cells

Answer 23

vascular endothelial cells (small blood cells)

Question 24

Once inside the cell Rickettsiae…

Answer 24

lyse phagosome (phospholipase) and enter cytosol

Question 25

R. prowazekii exits cell by

Answer 25

lysis

Question 26

R rickettsii exits cell by

Answer 26

using host actin to be pushed out of filopodia

Question 27

R. tsutsugamushi exits cell by

Answer 27

budding thru cell membrane (remains enveloped in host cell membrane as it infects other cells)

Question 28

Injury to host from Rickettsiae is dictated by

Answer 28

number of intracellular bacteria

Question 29

Rickettsiae symptoms

Answer 29

hemorrhagic spots from cell lysis

Question 30

R. prowazekki disease

Answer 30

Recrudescent typhus

Question 31

R. typhi disease

Answer 31

Murine typhus

Question 32

Why is diagnosing rickettsioses problematic?

Answer 32

no fever and not aware of tick bite

Question 33

Ricketts diagnostic tests

Answer 33

Antibody titers

Question 34

Mycoplasma significance

Answer 34

small organism capable of growth on cell free media

Question 35

Mycoplasma requires

Answer 35

sterol

Question 36

Mycoplasma appearance

Answer 36

fried egg small colonies slow growth

Question 37

Mycoplasma species

Answer 37

M. pneumoniae M. genitalium M. hominis Ureaplasma urealyticum

Question 38

True or false: some mycoplasma are part of normal human flora

Answer 38

True

Question 39

M. pneumoniae significance

Answer 39

Walking pneumonia | Primary atypical pneumonia: not cleared by penicillin

Question 40

M. Pneumoniae and penicillin

Answer 40

no meurin so not cured by penicillin