Lab 1 Flashcards
Leaves and Plant Reproduction (50 cards)
Midrib
large bulge in the middle of the leaf which contains a large vein. This midrib vein branches repeatedly into smaller and smaller veins extending throughout the leaf.
Veins
Vascular bundles of the leaf, primarily made up of vascular tissues XYLEM and PHLOEM.
Xylem cells
(stained red), are large empty cells with thick walls.
Phloem cells
(stained green), are smaller and have thinner cell walls.
Bundles Sheath
protective layer of parenchyma cells which typically encloses each vein.
Upper and Lower Epidermis
the outermost layer of the top and the bottom of the leaf respectively. Both layers are one cell thick and normally coated with a very thin waxy cuticle.
Cuticle Layer
waxy layer (thin or thick, transparent). Found above the upper epidermis and or below the lower epidermis.
Stomata
pores (holes) in the upper and or lower epidermis. Surrounded by a pair of guard cells, which can be open or closed to allow for gas exchange.
Hydrophytes
plants adapted to wet environments (hydro=water) (phyte=plant)
Mesophytes
plants adapted to moderate amounts of water in their habitats (meso=middle)
Xerophytes
plants adapted to dry environments (xero=dry)
Mesophyll
sandwiched between the upper and lower epidermis, the photosynthetic tissue that makes up the middle part of the leaf. It is best observed in the regions between the veins. Consists of two tissue types: palisade mesophyll ( or parenchyma) and spongy mesophyll (or parenchyma).
Palisade mesophyll
long rectangular cells filled with chloroplast (photosynthesis)
Spongy mesophyll
Irregular shaped cells, loosely packed (gas exchange).
Vascular bundles
composed of thick walled Xylem cells (transports water and minerals) and smaller thin walled Phloem cells (transports sugars).
Tissue plans
A line drawing to proportion to show the different “layers” of tissue only, without cellular details. Notes are very important, must show all formulas and calculations.
Lengths
- Diameter of field view under low power = 4500µm
- Diameter of field view under medium power = 1800µm
- Diameter of field view under high power = 450µm
Calibrated values for this semester
- Low power objective: 250 µm/od
- Medium power objective: 100 µm/od
- High power objective: 25µm/od
Nerium (xerophyte) internal adaptation to prevent water loss
- Crypts with epidermal hair
- Thickened cuticles on upper and lower epidermis
- Thickened upper epidermis and palisade mesophyll
Sample Calculations: Specimen Length and Specimen Width
Specimen Length: Diameter of field view under high power = 450µm
Specimen Width: Calibrated value for one ocular division for high power objective = 25µm/od = ?µm (value you calculate from the above line) X # of ocular divisions taken up by the specimen’s width #od (value you determine using the ocular ruler).
Scale calculation
Scale = Actual Specimen Length/ Length of Drawing (chosen between 7 and 10cm) = 450µm/*cm = **µm/cm
Drawing width
Drawing width = Actual Specimen Width/ Scale = ?µm/ **µm/cm = —cm
Tissue plan format
Figure 1: Tissue Plan of —–(viewed at—-)
Actual Specimen Length* Actual Specimen Width; Scale
DRAWING
Notes: 3-4 GOOD notes (full complete sentences):
- Drawn from prepared slide of temporary wet mount?
- Describe each layer: shape of cells? How many cells thick?
- Structures expected to see but didn’t?
Complete Flower
4 whorls present
- sepals
- petals
- stamen: anther & filament
- carpel/pistil: stigma, style, ovary & ovules