Lab 1 - Assaying Biomolecules Flashcards
What is usually quantified when a standard curve (calibration curve) is generated?
the amount or concentration of an analyte in a sample
Which relationship does a standard curve describe?
the relationship between signal intensity and amount/concentration of the analyte
What are the two steps that lead to color developpment because of RC DC?
1- Reaction between the copper and protein at alkaline (basic) pH
2- The reagent of the Folin agent by the copper-treated protein.
Why is the RC DC assay coloremetric?
AS the concentration of the protein goes up, the color of the samples become darker.
What is the purpose of using a control?
It generates data to which the experimental data can be compared. In th case of this lab, it creates a standard curve to which can be extrapolated unknown concentrations. The buffer calibrates the spectrophotometer, so all the data is relative to the buffer.
