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Lab Flashcards

1
Q

Aerobic bacteria(BMN)

A
  1. Bacillus subtilis
  2. Mycobacterium tuberculosis
  3. Norcadia asteroides
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2
Q

Anaerobic bacteria(VELA)

A
  1. Veillonella
  2. Eubacterium
  3. Lactobaccilus
  4. Actinomyces
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3
Q

Facultative anaerobes

A
  1. Staphylococcus aureus
  2. Escherichia Coli
  3. Listeria
  4. Cutibacterium acne
  5. Clostridium botulium
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4
Q

Micraerophiles (C.H.S)

A
  1. Campylobacter jejuni
  2. Heamophilus influenzae
  3. Streptococcus oralis
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5
Q

Aerotolerant (L.S)

A
  1. Lactobacillus rhamnosus
  2. Streptococcus pyrogens
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6
Q

Cocci bacteria

A
  1. Staphylococcus aureus
  2. Staphylococcus oxalis
  3. Streptococcus pyogens
  4. Streptococcus pneumoniae
  5. Neisseria gonorrhoea
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7
Q

Rod shaped bacteria

A
  1. E. Coli
  2. Bacillus anthracis
  3. Corynebacterium diphtheria
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8
Q

Vibrio bacteria

A
  1. Vibro cholerae
  2. Vibro harveyi
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9
Q

Spirillum shaped bacteria

A
  1. Spirillum volutans
  2. Helicobacter pylori
  3. Campylobacter jejuni
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10
Q

Gram negative bacteria (KESPP)

A
  1. Pseudomonas
  2. Klebsiella
  3. Escherichia
  4. Salmonella
  5. Citrobacter
  6. Morganella
  7. Aeromonas
  8. Providencia
  9. Proteus
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11
Q

Gram positive (MNSSCA)

A
  1. Mycobacterium
  2. Norcardia
  3. Streptococci
  4. Staphylococci
  5. Clostridium
  6. Actinomyces
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12
Q

Sources of nitrogen

A

Peptone, meat, amino acid, yeast

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13
Q

Carbon

A

Organic acids and sources

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14
Q

Minerals

A

NaCl is used to provide isotonic cdts

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15
Q

Nutrient broth components

A
  1. Lab lemco powder - 1g
  2. Yeast extract - 2g
  3. Peptone - 5g
  4. NaCl - 5g
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16
Q

Nutrient broth total amount

A

13g in 1000ml

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17
Q

Lab lemco powder

A

It has the ability to support bacteria growth

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18
Q

Yeast extract

A

It’s a rich source of amino acids, vitamins and minerals

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19
Q

Peptone

A

Makes nitrogen available in the form of amino acids

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20
Q

NaCl

A

It maintains the osmotic balance of the medium

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21
Q

Other solidifying agents

A

Pectin, silica gel ,silicone, starch, Xanthan gum, gelatin

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22
Q

Nutrient agar total amount

23
Q

Nutrient agar amount of water for dissolving

24
Q

Nutrient broth amount of water for dissolving

25
Concentration of Agar
It is usually used in concentrations of 2- 3%
26
Components of peptone
1. Nicotinic acid 2. Riboflavin 3. Peptones 4. Amino acids 5. Inorganic salts ( magnesium, calcium)
27
Disadvantages of liquid media
1. Growth in liquid media can not be ordinarily quantitated 2. Bacteria grown in liquid media form colloidal suspension 3. Identification of cultures in liquid media requires sub growth onto a solid media.
28
Examples of liquid media
Nutrient broth, peptone water, tryptone water, Koser's citrate medium, MRVP Medium, sugar medium
29
Examples of solid media
1. Nutrient agar 2. Blood agar 3. MacConkey agar 4. Mannitol salt agar 5. Bismuth sulphite agar 6. Cetrimide agar 7. Chocolate agar.
30
Semisolid agar
Prepared with the use of a reduced conc of agar ( 0.2- 0.4%)
31
Examples of semisolid media
1. Hugh and Leifson's oxidation fermentation medium 2. Stuart's and Amies media 3. Mannitol motility media
32
MacConkey agar
For cultivation of gram-negative bacteria (KESPP)
33
Blood agar
Can be used for fastidious organisms( mycobacterium tuberculosis)
34
Simple media
Media that serve as the basal media for other media .Eg nutrient broth and nutrient agar.
35
Complex media ( undefined media)
Media, which contains complex ingredients in unknown proportions
36
Defined media
This is a media that contains defined proportions of all ingredients. Eg Dubos' medium
37
Special media
1. Enriched media 2. Enrichment media 3. Selective media 4. Differential media or indicator media 5. Transport media 6. Sugar media
38
Enriched media
Mostly used for fastidious organisms. Are prepared by adding substances like blood,serum, or egg to simple media. Egs blood agar, chocolate media
39
Lactose fermenting bacteria like E. Coli
Form pink colonies on MacConkey agar
40
Non lactose fermenting bacteria like Salmonella sp
Form pale or colourless colonies on MacConkey agar
41
MacConkey agar
Inhibits Gram-positive cocci bacteria
42
Transport media
They are used to ensure the viability of microorganisms in clinical specimens during their transport to the lab
43
Transport media
They lack carbon, nitrogen, and other growth factors; hence, don't encourage multiplication. They contain only buffers and salt.
44
Sugar media
Contains 1% of sugar( carbs)
45
Exales of organisms that can test positive for hydrogen sulphide production test
E.Coli and Salmonella
46
Examples of organisms that can test positive for citrate utilisation test
Klebsiella aerogenes,klebsiella pneumonia, and Proteus mirabillis
47
Organisms that test negative for citrate utilisation
E.Coli and Shigella dysenteria
48
Organisms that test positive for indole production (EPV)
1. Escherichia coli 2. Proteus vulgaris 3. Vibrio cholerae
49
Organisms that test negative for indole production test(SKP)
Salmonella typi Klebsiella spp P.mirabi
50
Methyl red test
Used to distinguish between E. Coli(+ve) from Enterobacter(MR -ve). And Yerisinia +ve from other gram- negative non-enteric bacteria ( MR -ve)
51
Voges P
52
Cetrimide agar
It is selective for highly resistant bacteria like Pseudomonas aeruginosa and Mycobacterium tuberculosis.
53
Cetrimide agar ( Pseudosel agar or pseudomonas cetrimide agar)
It inhibits E. Coli and other gram neg and pos bacteria
54
Example of non-hemolytic bacteria
Vibrio cholerae

Micro Biology (7 decks)

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