LAB 2

Question 1

Used to observe minute organisms

Answer 1

MICROSCOPE

Question 2

Functions of Microscopes in Microbiology

Answer 2

1. Identify the features of the microorganisms
2. Characterize microorganisms
3. Determine the Biology of Microorganisms

Question 3

has 2 lenses and uses visible light

Answer 3

COMPOUND MICROSCOPE

Question 4

Objectives of the compound Microscope

Answer 4

SCANNER - 4X
LPO - 10X
HPO - 40X
OIL IMMERSION OBJECTIVE - 100X

Question 5

Magnification of ocular lenses

Answer 5

5x AND 10x

Question 6

Formula for calculating the total magnification

Answer 6

ocular x objectives

Question 7

STAINED AND UNSTAINED SLIDES

Answer 7

COMPOUND AND ELECTRON MICROSCOPE

Question 8

Illuminates a dark background; has a special type of condenser that scatters light

Answer 8

DARK FIELD MICROSCOPE

Question 9

Uses beam of light/electrons; Provides a high resolution of biological and non-biological samples

Answer 9

ELECTRON MICROSCOPE

Question 10

uses UV light; specific fluorescent dye; stained

Answer 10

FLUORESCENCE MICROSCOPE

Question 11

Observing live organisms in their natural state; unstained and has a special condenser

Answer 11

PHASE CONTRAST MICROSCOPE

Question 12

Illuminating parts

Answer 12

Iris Diaphragm
Condenser
Illuminator

Question 13

Uses coarse adjustment (maximized movement)

Answer 13

LPO and scanning

Question 14

Uses Fine adjustment (minimized movement)

Answer 14

HPO and Oil Immersion Objective

Question 15

distance of the objective to the stage

Answer 15

Working distance

Question 16

TRUE OR FALSE

The relationship between magnification and the working distance is inversely proportional

Answer 16

TRUE

Question 17

Mechanical Parts

Answer 17

Base
Arm
Nose Piece
Fine Adjustment
Coarse Adjustment

Question 18

Magnifying Parts

Answer 18

Ocular
Objectives

Question 19

Number that expresses the ability of a lens to receive fine detail in an object being observed.

Measure of the resolving power of an objective

Answer 19

NUMERICAL APERTURE

Question 20

Numerical aperture of OIO

Answer 20

1.25

Question 21

It’s NA is 0.25

Answer 21

Low Power Objective

Question 22

It’s Magnification is 4x and has an NA of

Answer 22

Scanning; 0.1

Question 23

HPO magnification and NA

Answer 23

40X and 0.65

Question 24

COLOR BANDS

SCANNING
LPO
HPO
OIO

Answer 24

SCANNING - Red
LPO - Yellow
HPO - Blue
OIO - White

Question 25

# TRUE OR FALSE MAGNIFICATION IS INVERSELY PROPORTIONAL TO THE NUMERICAL APERTURE

Answer 25

FALSE

Question 26

Inversely proportional to numerical aperture; ACCEPTABLE SHARPNESS

Answer 26

DEPTH OF FOCUS

Question 27

Inversely proportional to numerical aperture, but directly proportional to the depth of field;

Answer 27

RESOLUTION

Question 28

Inversely proportional to numerical aperture; Scope

Answer 28

Depth of Field

Question 29

constant image even if the objective was changed

Answer 29

PARFOCAL

Question 30

houses the optical parts in the upper part of the microscope

Answer 30

HEAD/BODY

Question 31

supports the microscope and houses the illuminator

Answer 31

BASE

Question 32

connects to the base and supports the microscope head. It is also used to carry the microscope

Answer 32

ARM

Question 33

what you look through at the top of the microscope

Answer 33

EYEPIECE

Question 34

holds the eyepieces in place above the objective lens

Answer 34

EYEPIECE TUBE

Question 35

primary optical lenses on a microscope; ranging from 4x to 100x

Answer 35

OBJECTIVE LENSES

Question 36

Houses the objectives; used to select the objective

Answer 36

Nosepiece

Question 37

where the specimen to be viewed is placed; used when working at higher magnifications where delicate movements of the specimen slide are required.

Answer 37

STAGE

Question 38

when there is no mechanical stage

Answer 38

STAGE CLIPS

Question 39

hole in the stage through which the base (transmitted) light reaches the stage

Answer 39

APERTURE

Question 40

used to collect and focus the light from the illuminator on to the specimen

Answer 40

CONDENSER

Question 41

controls the amount of light reaching the specimen

Answer 41

IRIS DIAPHRAGM

Question 42

moves the condenser up or down to control the lighting focus on the specimen

Answer 42

CONDENSER FOCUS KNOBS

Question 43

optical constant of the lens

Answer 43

FOCAL LENGTH (mm)

Question 44

distance from the center of the lens to the point where parallel rays entering the lens are brought to a focus

Answer 44

FOCAL LENGTH (mm)

Question 45

free space between the specimen surface and the objective

Answer 45

WORKING DISTANCE (mm)

Question 46

Measure of the resolving power of an objective

Answer 46

NUMERICAL APERTURE

Question 47

the property to recognize features of a specimen to each other as separate and distinct

Answer 47

RESOLVING POWER OF AN OBJECTIVE

Question 48

The objectives are optically and mechanically designed so that the distance between the specimen and the aerial image is always constant

Answer 48

PARFOCAL

Question 49

It is used in refocusing and resolve the sharpness of the image after changing from one objective to another

Answer 49

FINE FOCUS KNOB

Question 50

# TRUE OF FALSE: Place the microscope close to the edge of the table.

Answer 50

TRUE

Question 51

It is used to lower the body tube until the 4X or 16mm objective reaches the downward stop.

Answer 51

COARSE FOCUS KNOB

Question 52

The circular area seen in the eyepiece

Answer 52

FIELD OF VISION

Question 53

It is used for initial focusing and viewing.

Answer 53

SCANNING OBJECTIVE

Question 54

# TRUE OR FALSE The higher the magnification, the lower the depth of focus.

Answer 54

TRUE

Question 55

# TRUE OR FALSE: The higher the power of objective, the lesser is the area of the specimen surface included in the field of vision.

Answer 55

TRUE

Question 56

Thickness of the layer within the boundaries of which all points appear to be sharply in focus

Answer 56

Depth of Focus

Question 57

# TRUE OF FALSE: The depth of focus is inversely proportional to the square of the numerical aperture.

Answer 57

TRUE