Lab #2 Flashcards
Microscopes all have
Lens system, adjustable light source, and focusing mechanism
The base of a compound microscope contains
Light switch, light source, and voltage adjustment controls
What does the condenser do?
Focuses the light on the specimens on the slide supported on the microscope stage
An iris diaphragm allows
To regulate light intensity
Glass slide is where
The specimen is mounted
Stage clips help
Hold slide in place
Mechanical stage control allows you to
Move the mechanical stage
Light pass through the
Objective lens and passes through the ocular lens
Monocular
One ocular lens
Binocular
Two ocular lenses
Coarse focusing knob
Rough focus
Fine-focusing knob
Sharp focus
Compound microscope is used to..
Observe microscopic objects
Magnification
Indicates the number of times the image of the sample is enlarged by the microscope
Ocular lens have a magnification power of
10x
Resolution is
The ability to clearly distinguish objects which are close together by decreasing the wavelength of light used to increase resolution
Specimen preparation
Mounting of sample on the microscope slide with a coverslip
Prepared/permanent slides
Preserved living samples
Wet mount/temporary slides
Aqueous solution covered with a cover glass
Focusing
Rotating focus knobs to adjust distance
Field of view
Diameter of visible image
Depth of field
Distance between highest and lowest points in the sample that are simultaneously in focus
Working distance
Distance between objective and specimen
Light intensity, working distance, field of view, and depth of field ______ when magnification increases
Decrease
