Lab 3 (a and b) Flashcards
(43 cards)
what is a round cell shape called
coccus
what are the different cell arrangements for round cells
- single cocci
- diplococci
- stroptococci (line)
- staphylococci (clumps)
what is a rod cell shape called
bacillus “rod shaped”
what are the different cell arrangements for chain shaped cells
- single bacilli
- streptobacilli (chain)
what is a spiral-shaped cell called
spirillum
what are the different cell arrangements for spiral shaped cells
- single spirillum
what are the different colony morphology characteristics
- colony color (white/tan)
- colony shape (circular/non circular)
- colony texture : rough/smooth
- light reflection : matte/glossy
- colony transparency : translucide/opaque
what is the e.coli and B subtilus starch agar test
e coli = negative
B subtilus = positive (produces amylase, breaks down starch)
what are the 4 antibiotics used in the lab to test inhibition
penicilin
norfloxacin
streptomycin
Cefotaxime
what is e coli sensitivity to penicilin
not sensitive (-) 6 mm
what is e coli sensitivity to cefotaxime
medium sensitivity (++)
what is e coli sensitivity to norfloxacin
high sensitivity
what is e coli sensitivity to streptomycin
medium sensitivity
what is B.sub sensitivity to cefotaxime
medium
what is B.sub sensitivity to pen
medium
what is B.sub sensitivity to nerfloxacin
high
what is B.sub sensitivity to strepto
medium
what are the zones of inhibition for each sensitivity
*all in mm
- 6 = not sensitive (-)
- 6 - 10 = low sensitivity
- 10 - 25 = medium
- more then 25 = high
which antibiotic has the largest inhibition diff between both
penicilin
what is the target of pen
inhibition of cell wall synthesis
what is the target of nerfloxacin
DNA rep
what is the target of cefotaxime
peptidoglycan/cell wall synthesis
what is the target of streptomycin
protein synthesis
what is e coli cell arrangement
single bacillus
