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Biology lab test > Lab 3 (a and b) > Flashcards

Lab 3 (a and b) Flashcards

1
Q

what is a round cell shape called

A

coccus

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2
Q

what are the different cell arrangements for round cells

A
  • single cocci
  • diplococci
  • stroptococci (line)
  • staphylococci (clumps)
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3
Q

what is a rod cell shape called

A

bacillus “rod shaped”

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4
Q

what are the different cell arrangements for chain shaped cells

A
  • single bacilli
  • streptobacilli (chain)
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5
Q

what is a spiral-shaped cell called

A

spirillum

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6
Q

what are the different cell arrangements for spiral shaped cells

A
  • single spirillum
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7
Q

what are the different colony morphology characteristics

A
  • colony color (white/tan)
  • colony shape (circular/non circular)
  • colony texture : rough/smooth
  • light reflection : matte/glossy
  • colony transparency : translucide/opaque
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8
Q

what is the e.coli and B subtilus starch agar test

A

e coli = negative
B subtilus = positive (produces amylase, breaks down starch)

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9
Q

what are the 4 antibiotics used in the lab to test inhibition

A

penicilin
norfloxacin
streptomycin
Cefotaxime

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10
Q

what is e coli sensitivity to penicilin

A

not sensitive (-) 6 mm

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11
Q

what is e coli sensitivity to cefotaxime

A

medium sensitivity (++)

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12
Q

what is e coli sensitivity to norfloxacin

A

high sensitivity

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13
Q

what is e coli sensitivity to streptomycin

A

medium sensitivity

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14
Q

what is B.sub sensitivity to cefotaxime

A

medium

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15
Q

what is B.sub sensitivity to pen

A

medium

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16
Q

what is B.sub sensitivity to nerfloxacin

A

high

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17
Q

what is B.sub sensitivity to strepto

A

medium

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18
Q

what are the zones of inhibition for each sensitivity

A

*all in mm
- 6 = not sensitive (-)
- 6 - 10 = low sensitivity
- 10 - 25 = medium
- more then 25 = high

19
Q

which antibiotic has the largest inhibition diff between both

A

penicilin

20
Q

what is the target of pen

A

inhibition of cell wall synthesis

21
Q

what is the target of nerfloxacin

A

DNA rep

22
Q

what is the target of cefotaxime

A

peptidoglycan/cell wall synthesis

23
Q

what is the target of streptomycin

A

protein synthesis

24
Q

what is e coli cell arrangement

A

single bacillus

25
Q

what is B. subtilus cell arrangement

A

strepto bacillus

26
Q

what is S. aureus cell arrangement

A

staphylo coccus

27
Q

what is S. Volutans cell arrangement

A

single spirillum

28
Q

which of the 4 bacteria have flagella

A

S. volutans

29
Q

what is e coli colony shape

A

circular

30
Q

what is B sub colony shape

A

non-circular

31
Q

what is e coli colony pigmentation

A

tan

32
Q

what is B.sub colony pigmentation

A

white

33
Q

what is b sub colony texture

A

rough

34
Q

what is e coli colony texture

A

smooth

35
Q

what is e coli colony reflection

A

glossy

36
Q

what is b sub colony reflection

A

matte

37
Q

what is e coli colony transperency

A

translucent

38
Q

what is b sub colony transperency

A

opaque

39
Q

where is e coli found

A

intestine

40
Q

where is b sub found

A

soil

41
Q

What is viewed at cell level

A
  • gram stain
  • cell shape (bacillus, coccus, spirilla)
42
Q

What is viewed at molecular level

A
  • metabolic capacities : amylase with iodine
  • sensitivity to antibiotics
43
Q

What is viewed at colony level

A
  • colony morphology (mass of cells derived from a single isolated cell during inoculation on agar medium)

Biology lab test (6 decks)

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