Lab Flashcards
(40 cards)
Phenol Red Broth
(+) Yellow broth, bubble in tubeA/G
(+) Yellow broth, no bubbleA/–
(-) Red broth, no bubble* –/–
(-) Pink broth, no bubble *K
Methyl Red & Voges-Proskauer
Red *(+)
No Color Change *(–)
Catalase Test
Bubbles *(+)
No Bubbles *(–)
Oxidase Test
Dark Blue/Purple within 20/s*(+)
No color change to Blue/Purple within 20/s *(–)
Nitrate Reduction Test
- Gas (non-ferm) *(+)
- Gas (ferm, or status unknown)
- Red color (after Reagents A&B) *(+)
- No color change(after Reagents A&B)
- No color change(after zinc dust) *(+)
- Red color(after zinc) *(–)
Citrate Utilization Test
Blue(even small amount) *(+)
No color change; growth *(+)
No color change; no growth *(–)
Malonate Utilization Test
Dark Blue *(+)
No color change; or slightly yellow *(–)
Decarboxylation Test (Ornithine)
Purple ( may be slight) *(+)
No color change *(–)
Yellow *(–)
Phenylalamine Deaminase Test
Green color *(+)
No color change *(–)
Starch Hydrolysis (Amylase Test)
Clearing around growth *(+)
No clearing around growth*(–)
Gelatin Hydrolysis
Gelatin is liquid (control is solid) *(+)
Gelatin is solid *(–)
Urea Hydrolysis
24 hours: 1.All pink *(+) 2.Partially pink *(w+) 3.Orange or yellow *(w+) 4.Orange or yellow*(--) 24h to 6d: 1.Gelatinase is present *(+) 2.All pink or Partially pink *(w+) 3.All pink or partially pink *(w+) 4.Orange or yellow*(--)
Indole Production
Layer on top of media turns Red/Pink *(+)
Reagent color is unchanged *(–)
Kliger Iron Agar (KIA)
- Yellow slant/yellow butt *(A/A)
- Red slant/yellow butt
* (K/A) - Red slant/red butt
* (K/K) - Red slant/ no change in butt
* (K/NC) - No change in slant/no change in butt *(NC/NC)
- Black precipitate in agar *(H2S)
- Cracks in or lifting of agar
Litmus Milk Medium
1.Pink color *(A)
2.Pink and solid (white in the lower portion if litmus is reduced) clot not movable *(AC)
3.Fissures in clot *(G)
4.Clot broken apart *(S)
5.White color (lower part of medium) *(R)
6.Semisolid and not pink;
clear to grey fluid on top *(C)
7.Clarification of medium; loss of body *(D)
8.Blue medium or blue band on top *(K)
9.No change *(NC)
Enterotube II Interpretation
- Add the circled numbers for each groups up and write the sum on the corresponding lines
(e. g. if the enterotube showed a + reaction for glucose and gas then you write a number 2 + 1 = “3” on the first line) - The established ID code identifies the bacterium under investigation with the help of the Coding Manual
Why use Enterotube?
The Enterotube is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae.
Flagella:
Structure that allows bacteria to move
Motility test and its media:
Semisolid media that contains TTC and less agar to make it more gelatinous. TTC is salt that becomes oxidized when exposed to air.
Aerobic/ Anaerobic/ Facultative
Aerobic: require oxygen
Anaerobic: do not require oxygen
Facultative: can grow in both environments but prefer oxygen.
GasPak/ Anaerobic chamber
made of metal and will work as a catalyst. Takes O2 that is floating and takes O2 Hydrogen to catalyze them by making them water.
Spectrophotometry
An apparatus for measuring intensity of light in a part of the spectrum, as transmitted or emitted by particular substances.
What is the plate count method?
The plate count method means diluting bacteria with a diluent solution (e.g. sterile saline) until the bacteria are dilute enough to count accurately when spread on a plate. The assumption is that each viable bacterial cell will develop into a single colony.
Absorbance method:
The reason why we use it
Purpose
Advantages/disadvantages
Measure the rate of growth of bacterial culture.
