lab 7: gel electrophoresis Flashcards
1
Q
restriction digestion
A
treat DNA with restriction enzymes
2
Q
gel electrophoresis
A
DNA into agarose (gel), apply electric current, drags DNA through gel, sort by size, visualize DNA fragments
3
Q
restriction endonuclease
A
enzyme binding to DNA at specific nucleotide sequence, cuts both strands of sugar phosphate backbone
4
Q
restriction site
A
positions cut on DNA molecule by enzyme
5
Q
agarose
A
polysaccharide
6
Q
phosphate
A
negative
7
Q
smaller fragments
A
faster, longer down gel
8
Q
larger fragments
A
slower, less far down gel
9
Q
markers
A
DNA fragments of known lengths, compares size
10
Q
ethidium bromide
A
emits orange when exposed to UV light
11
Q
AVI
A
has Fok1 site
12
Q
A
