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Bacteriology > Lab 7: Pasteurella, Mannheimia, Bibersteinia, Ornithobacterium, Riemerella, Actinobacillus, Haemophilus, Histophilus, Avibacterium, Taylorella > Flashcards

Lab 7: Pasteurella, Mannheimia, Bibersteinia, Ornithobacterium, Riemerella, Actinobacillus, Haemophilus, Histophilus, Avibacterium, Taylorella Flashcards

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1
Q

Genera within Pasteurellaceae family?

A
  1. Pasturella
  2. Mannheimia
  3. Bibersteinia
  4. Actinobacillus
  5. Haemophilus
  6. Avibacterium
  7. Histophilus
  8. Gallibacterium
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2
Q

other genera resembling pasteurellaceae?

A
  1. Ornithobacterium
  2. Reimerella
  3. Taylorella
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3
Q

general habitat of pasteurellaceae?

A

Mucous membranes

(respiratory tract, genitals, alimentary tract)

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4
Q

general morphology of pasteurellaceae?

A

0.5-2μm coccoid rods

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5
Q

general staining of pasteurellaceae?

A
  • Gram - negative
  • Bipolar staining
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6
Q

what is bipolar staining?

A

more intense staining in the middle of the bacteria

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7
Q

what is the general culture of pasteurellaceae?

A
  • Fastidious (Yeast extract, blood, PH-sensitivity)
  • aerobic - facultative anaerobic
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8
Q

general biochemistry for pasteurellaceae?

A
  • oxidase +
  • catalase +
  • Fermentitative
  • toxin production by some species
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9
Q

Pasteurellaceae antigens?

A

cell wall and capsule antigens

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10
Q

pasteurellaceae resistance?

A

Generally weak resistance

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11
Q

Pathogenicity of pasteurellace genera?

A

Facultative pathogens

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12
Q

habitat of pasteurella genera?

A

mucous membranes of the respitatory tract

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13
Q

pasteurella genus produces a capsule?

A

True

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14
Q

colony types of pasteurella?

A

M-S-R colonies

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15
Q

types of cyclotoxins produced by pasteurella?

A

P. multocida D - dermonecrotoxin

  • causing damage to osteoblasts
  • biochemical differenced between P. multocida spp.
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16
Q

P. multocida antigens?

A
  • cell wall antigens
    • 16 Heddleston system
    • (11: namioka system)
  • capsule antigens
    • A,B,E,D,F
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17
Q

species of Pasteurella?

A
  1. P. Multocida A,D
  2. P. multocida D
  3. P. multocida B,E
  4. P. pneumotropica
  5. P. aerogenes
  6. P. canis, P. dagmatis, P. stomatis
  7. P. Caballi
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18
Q

Effects of P. multocida A,D

A
  • Ruminant and swine pneumonia
  • Rabbit septicaemia and pneumonia
  • Fowl cholera
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19
Q

what are the effects of P. multocida D?

A

Atropic rhinitis of swine

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20
Q

what are the effects of P. multocida B,E?

A

Haemorrhagic septicaemia

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21
Q

Habitat of Mannheimia, Biebersteinia?

A

Mucous membranes (respiratory tract)

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22
Q

Mannheimia and Biber steinia cause ß haemolysis?

A

True

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23
Q

toxins produced by mannheimia and biersteinia?

A

leukotoxin production

  • damage of macrophage and endothelial cells
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24
Q

antigens of mannheimia and biersteinia?

A
  • call wall antigens cannot be used for diagnostics
  • capsule
    • M. haemolytica 12 types
    • B. trehalosi 4 types
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25
pathogenicity of mannheimia and Beibersteinia?
1. **M. Haemolytica** * Pasteurellosis * ruminant pneumonia * Lamb septicaemia * mastitis * arthritis 2. **B. Trehalosi** * acute systemic pasteurellosis * feed-lot lambs
26
Actinobacilllus habitat?
Alimentary tract, respiratory and genital tract, mucous membranes
27
Species of Actinobacillus?
1. A. lignieresii 2. A. equuli 3. A. suis 4. A. seminis 5. A. ureae 6. A. Pleuropneumoniae
28
habitat of A. lignieresii?
cattle and sheep oral cavity, rumen and gut
29
morphology of A. lignieresii?
* 1 - 1.2μm rods * filaments * sulphur granules in lesions (like actinomycosis)
30
culture of A. lignieresii?
* aerobic, facultative anaerobic * poor growth on nutrient agar * sticks to the agar
31
Antigens of A. lignieressi?
Cell wall antigens * 6 serogroups
32
pathogenicity of A. lignieressi?
cattle, sheep and swine **actinobacillosis** * **Wooden tongue** * granulocytomas * pus containing small, hard yellow to white granules
33
A. equuli habitat?
horse and swine alimentary tract, tonsils, respiratory tract
34
A. equuli has filaments?
true
35
A. equuli cultures?
* large viscous colonies * some strains can haemolyse
36
subspecies of A. equuli?
1. A. equuli subsp. equuli 1. A. equuli subsp. haemolyticus
37
both A. equuli subspecies cause which disease?
actinobacillosis
38
symptoms of actinobacillosis in the foal?
septicaemia, nephritis, pneumonia, arthritis
39
habitat of A. suis?
Swine mucous membranes respiratory and genital tract
40
culture of A. suis?
* Blood agar * ß hemolysis
41
pathogenicity of A. suis?
* Swine (grower/adult) * pneumonia, septicaemia, metritis, abortion, arthritis * Suckling piglet * septicaemia
42
habitat of A. seminis?
mucous membrane of genital tract of rams
43
A. seminis culture?
10% CO2 blood agar
44
A. seminis pathogenicity?
facultative pathogenic inflammation of the accessory glands epidiymitis abortion
45
habitat of A. ureae?
Human respiratory tract
46
habitat of A. pleuropneumoniae?
mucous membranes of the respiratory tract of swine
47
some strains of A. Pleuropneumoniae have fimbria?
true
48
culture for A. pleuropneumoniae?
* blood agar, chocolate agar with yeast extract * ß hemolysis
49
biotypes of A. pleuropneumoniae?
* Byotype 1: V - factor demand * satellitism, NAD, fresh yeast extract * Byotype 2: V factor is not needed
50
what is factor V?
NAD
51
cytotoxins produced by A. pleuropneumoniae?
I - II - III - IV cytotoxins produced * damage of macrophages and endothelial cells
52
how many serogroups of antigens does A. pleuropneumoniae have?
18 serotypes
53
what is the pathogenicity of A. pleuropneumoniae?
**swine hemorrhagic - necrotic pleuropneumonia**
54
habitat of Haemophilius
mucous membranes of human and animals | (respiratory and genital)
55
Haemophilus genus have filaments and sometimes fimbria?
true
56
Additives to the culture for Heamophilus?
* Haem (X - factor) * NAD (V - factor) * some species need 10-15% CO2
57
species of haemophilus?
**H. influenzae** **H. Parasuis**
58
features of H. parasuis?
* V factor demand * 15 serotypes * causes **Porcine polyserositis** (**glasser's disease**) * causes severe systemic disease characterised by: * Arthritis * Meningitis * Fibrious polyserositis
59
Habitat of Histophilus somni?
Urogenital and respiratory mucous membranes
60
culture requirements for H. somni?
* X, V factor not needed * CO2​ demand
61
pathogenicity of H. somini?
* cattle * calf pneumonia * septicaemia * thromboembolic meningoencephalitis (**TEME**) * Abortion * Mastitis * Sheep * epididymitis - orchitis * abortion * mastitis * lamb septicaemia
62
Habitat of Avibacterium?
respiratory mucosa
63
Avibacterium have filaments?
true
64
culture requirements of Avibacterium?
* some species need CO2 * V factor demand is variable
65
species of Avibacterium?
1. **A. paragallinarum** 2. **A. gallinarium** 3. **A. Avium**
66
features of A. paragallinarum?
* CO2 and factor V are needed * 3 serogroups, 9 subgroups * **Infectious coryzoa of hens** * **​**inflammation of sinuses, nasal discharge and sneezing * chicken cold - v. infectious
67
Features of A. gallinarum?
* factor V is not needed * mild respiratory disease of hens
68
features of A. Avium?
* Factor V is needed * saprophyte, sometimes causes mild respiratory disease in hens
69
habitat of orinithobacterium?
upper respitatory tract of some bird species (chicken and turkey)
70
culture needed for Orinthobacterium?
* blood agar * 10% CO2
71
biochemistry of orinithobacterium?
* Catalase - * oxidase +
72
pathogenicity of orinithobacterium?
turkey, chicken, upper respiratory infection
73
habitat of Riemerella anatipestifer?
water fowl upper respiratory ways
74
culture for Riemerella anatipestifer?
* blood agar * chocolate agar * 10% CO2
75
biochemistry of Rimierella anatipestifer?
* extracellular enzymes * proteolytic
76
pathogenicity of Riemerella anatipestifer?
**anatipestifer disease** in waterfowl
77
Habitat of Taylorella equigenitalis?
* genital mucous membranes of the stallion * clitoris of the mare
78
Taylorella equigenitalis has filaments?
true
79
culture needed for taylorella equigenitalis?
* X and V factors are not needed * CO2 demand
80
disease caused by taylorella equigenitalis?
**CEM: contagious equine metritis**

Bacteriology (2 decks)

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