Lab Diagnosis Flashcards
How can we clinically process samples for recovery?
Macro/Microscopic examination using gram stain
Plate and media inoculation
Incubation of anaerobic inoculated media
What produces a fluorescence under long wave UV and appears as brick red?
Porphyromonas and Prevotella
Pigmented species
Who recommends gram stains on all anaerobic specimens?
Clinical Laboratory Standards Institute (CLSI)
What can indicate necrotizing toxins?
Absence of leukocytes
What supports growth of all obligate and facultative anaerobes and is best for anaerobic gram positive cocci?
Anaerobic Blood Agar (CDC)
What differs the Brucella blood agar (BRU/BA) from the Anaeronic blood agar (CDC)?
Casein peptones
Dextrose
Hemin
What is selective and differential for the recovery and identification of C. difficile?
Cycloserine cefoxitin fructose agar (CCFA) plate
Yellow ground-glass colonies
What are most ideal for incubating anaerobic media?
Anaerobic chambers
Glove boxes are used
What is the length of time and temperature for anaerobic chambers?
5-7 days
35C - 37C
Why do we use glove boxes?
They are less susceptible to oxygen contamination than gloveless chambers
What can indicate the presence of anaerobes in cultures?
Foul odor
Colony morphology
Double zone hemolysis (SBA)
Good growth (BBE)
Brick red fluorescence (KVLB)
Which organisms can grow on the CO2 chocolate but grow better on anaerobic incubation plate?
Actinomyces
Clostridium
Lactobacillus
Propionibacterium
What media do Lecithinase and Lipase reactions use?
Egg yolk agar (EYA)
What can special potency disks be used for?
To determine the true gram stain reaction of the isolate
How do we expect Peptostreptococcus anaerobius and Peptoniphilus asaccharolyticus to respond to Sodium polyanethol sulfonate (SPS) disk?
P. anaerobius: Susceptible
P. asaccharolyticus: Resistant
