Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Mycology-Virology > Lab Dx Mycology > Flashcards

Lab Dx Mycology Flashcards

(68 cards)

Start Studying
0
Q

It is recommended that specimens should be stored under refrigeration at what temperature?

A

4degreesC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

All specimens should be collected _______ and placed in sterile containers.

A

aseptically

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

This should be stored at 30-37degreesC

A

Blood and CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

This should be stored at 15-30degreesC

A

Dermatologic specimens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Collection notes for BLOOD

A

Wright’s or Giemsa stain bottles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Isolation media for BLOOD

A

Sodium polyethanol sulfonate (SPS)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Collection notes for BONE MARROW

A

Heparin as anticoagulant and collect 0.5 ml of marrow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Isolation media for BONE MARROW

A

BHI broth, SDA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Collection notes for CSF

A

> 2ml: centrifugation

<2ml: uncentrifuged specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Isolation media for CSF

A

SDA, BHI with blood

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Collection notes for HAIR

A

Hairs that fluoresce or are broken and scaly

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Isolation media for HAIR

A

SDA, SDA-CC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Collection notes for NAILS

A

Scrape dissolved or hyperkeratotic areas

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Isolation media for NAILS

A

SDA, SDA-CC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Collection notes for SKIN

A

Scrape outer edge of ring in ringworm infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Isolation media for SKIN

A

SDA, SDA-CC, SDA with oil for Malassezia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Collection notes for MUCOCUTANEOUS

A

Scraping plaque with tongue depressor if Candida is suspected

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Isolation media for MUCOCUTANEOUS

A

SDA, SDA-CC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Collection notes for SUBCU TISSUE, LESIONS AND ABSCESSES

A

Biopsy or needle aspiration and examine presence of granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Isolation media for SUBCU TISSUE, LESIONS AND ABSCESSES

A

SDA, SDA-CC, anaerobic BHI with blood

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Collection notes for SPUTUM, BRONCHIAL WASHINGS AND TRANSTRACHEAL ASPIRATES

A

Early morning sputum for 3 consecutive days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Isolation media for SPUTUM, BRONCHIAL WASHINGS AND TRANSTRACHEAL ASPIRATES

A

SDA, BHI with blood and CC, anaerobic BHI with blood

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Collection notes for THROAT

A

2 sterile swabs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Isolation media for THROAT

A

SDA, SDA-CC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Collection notes for URINE
Midstream clean catch and detect fungal elements
25
Isolation media for URINE
SDA, SDA-CC
26
Collection notes for VAGINAL, CERVICAL
2 swabs examine with KOH
27
Isolation media for VAGINAL, CERVICAL
SDA, SDA-CC
28
Source of culture media
Nitrogen and carbon
29
What does fungi prefer for its growth?
Moisture and increased humidity
30
How many weeks should a culture be incubated?
2-4 weeks
31
Yeasts grow within _______ while H. capsulatum require _________ weeks to grow
24 to 72 hours... 10-12
32
Give the four Primary Isolation Media
SDA, BHI, DTM, Mycosel/Mycobiotic Agar
33
General isolation medium and it contains peptone and glucose
Sabouraud's Dextrose Agar (SDA)
34
Primary recovery of saprobic and pathogenic fungi
SDA
35
Isolation of saprophytic and pathogenic fungi from sterile sites especially SYSTEMIC MYCOSES
BHI
36
Useful for pathogenic fungi exclusive for dermatophytes
BHI
37
For the recovery of fungi from BLOOD and BONE MARROW
BHI Biphasic Culture Bottles
38
Isolation of dermatophytes from hair, skin and nail specimens
DTM
39
Indicator that it has changed to alkaline pH
Yellow to red
40
Inhibitory agents: cycloheximide and chloramphenicol. This is similar to DTM
Mycosel/Mycobiotic Agar
41
This is a differential culture media wherein it shows stimulation of conidiation and chlamydospore production in Candida spp
Cornmeal Agar with Tween 80
42
Useful for spp ID of Candida
Cornmeal Agar with Tween 80
43
Cornmeal agar: numerous chlamydospores with flight clusters of blastoconidia
C. albicans
44
Cornmeal agar: fewer blastoconidia and no chlamydospores present
C. tropicalis
45
Cornmeal agar: colonies appear as "spiders"
C. parasilopsis
46
Cornmeal agar: elongated blastoconidia in a "log in a stream" arrangement
C. pseudotropicalis
47
Differential culture media for the isolation of C. neoformans
Birdseed/Niger seed Agar
48
This production breaks down the medium resulting to melanin forming black brown colonies
Phenol Oxidase
49
Differential culture media useful in slide cultures
Potato Dextrose Agar
50
Differential culture media which demonstrates pigment production of T. rubrum
PDA
51
Differential culture media for the detection of Urease producrion by C. neoformans
Urea Agar
52
Differenti culture media which differentiates T. rubrum from T. mentagrophytes
Urea agar
53
They can grow as white patches with glossy surface
Candida
54
They often appear whitish grey with fuzzy edges
Molds
55
Used in screening for keratinized tissue
KOH mount
56
Most accurate method to preserve and observe fungal microstructure
Slide culture
57
Slide culture should not be performed on ______, _______, and ________.
Coccidioides, Histoplasma, Blastomyces
58
To identify the capsule of C. neoformans
India ink
59
Used to visualize microscopic fungal morphology by imparting a blue color to the cell walls
LPCB
60
A brightening agent can bind to the chitin in the fungal cell wall providing contrast when examined under fluorescence xscope
Calcofluor white
61
Used for the detection of Intracellular Histoplasma capsulatum; C. neoformans also stains quite well in this mtd
Giemsa or Wright's stain
62
Used to screen fungi in histologic spx; fungi is outlined black against pale blue background
Methenamine-Silver Nitrate Stain
63
Stains hyphae of molds and some yeasts
PAS
64
Hydrolyzes CHO of cell walls of the org to form aldehydes and then react w fuchsin dye to form pink-purple complex
Periodic Acid
65
Differential test specific for C. albicans
Germ tube test
66
Differential test for specie ID of some yeasts; INOSITOL:C. Neoformans vs Rhodotorula
CHO Assimilation Test for Yeast
67
Differential test specific for Cryptococcus spp
Rapid Urease Test

Mycology-Virology (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions