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MicroLAB > Lab Exam #1 > Flashcards

Lab Exam #1 Flashcards

(58 cards)

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1
Q

Spore staining. What is the primary stain?

A

Malachite green

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2
Q

Spore staining. What is the mordant?

A

Steam

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3
Q

Spore staining. What is the decolorizer?

A

distilled water

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4
Q

Spore staining. What is the counterstain?

A

Safranin

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5
Q

Spore staining. What are the two genera common spore formers?

A

Bacillus and Clostridium

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6
Q

Spore staining. What color are endospores at the end of this stain?

A

green

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7
Q

Spore staining. What appears to be pink at the end of this stain?

A

vegetative cells

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8
Q

Gram staining. Primary stain?

A

Crystal violet

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9
Q

Gram staining. What is the mordant?

A

Gram’s iodine

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10
Q

Gram staining. What is the decolorizer?

A

Acid alcohol

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11
Q

Gram staining. What is the counterstain?

A

Safranin

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12
Q

Gram staining. Which of the chemicals used in the staining procedure that we did in lab does not match the book? (Read the bottle labels in your lab)

A

Acid-alcohol and carbol fuchsin

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13
Q

Gram staining. What color is a gram positive cell at the end of the stain?

A

Purple (staphylococcus)

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14
Q

Gram staining. What color is a gram negative cell at the end of the stain?

A

red (Escherichia)

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15
Q

Gram staining. List the shapes and colors of the three genera we used for gram staining

A
  1. Escherichia coli, pink rods 2. Staphylococcus. Purple spheres 3. Bacillus badius. Pink-red rods
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16
Q

Gram staining. Identify Gram stain versus other staining procedure

A

Gram stain will have purple spheres (crystal violet) whereas your acid fast stain will have blue spheres(methylane blue)

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17
Q

Acid-fast staining. What is the primary stain?

A

Carbol fuchsin

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18
Q

Acid-fast staining. For the primary stain, what is the new name when basic fuchsin is mixed with phenol and other non polar solvents?

A

Carbol fuchsin

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19
Q

Acid-fast staining. Which chemical in the primary stain acts as the mordant?

A

Phenol

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20
Q

Acid-fast staining. What is the decolorizer?

A

acid alcohol

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21
Q

Acid-fast staining. What is the counterstain? How long did we need to counterstain for to ensure that the contrasting color was visible?

A

Methylene blue;

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22
Q

Acid-fast staining. What color are acid-fast positive cells at the end of the stain?

A

pink-red

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23
Q

Acid-fast staining. What color are the acid-fast negative cells at the end of the stain?

A

blue

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24
Q

Acid-fast staining. What is the genus of the acid-fast organism used in lab?

Study These Flashcards
A

Mycobacterium

25
Acid-fast staining. What is the genus of the organism that was used as a negative control?
Staphylococcus
26
Acid-fast staining. Identify acid-fast staining versus other staining procedures
Slide will be clumps of pink rods and blue-purple spheres
27
Capsule staining. How is the basis of a negative stain different from the other staining protocols that we performed so far in lab?
We are staining the background of the slide instead of the organism
28
Capsule staining. How is the fixing step for the capsule stain different from the other staining protocols that we performed so far in lab?
Instead of fixing it with heat, we are fixing it with acid alcohol
29
Capsule staining. What is the primary stain?
Congo red
30
Capsule staining. What is the purpose of the acid-alcohol for the capsule stain? (How does this compare to the use of acid-alcohol for the acid-fast stain?)
The acid alcohol chemically fixes the slide. In acid fast staining, acid alcohol is used as a decolorizer.
31
Capsule staining. How do capsules appear at the end of the staining procedure?
They appear clear with a rod-shaped bacteria in the capsule that can appear red-pink in color
32
Capsule staining. What color is the background?
gray, purple, or red
33
Capsule staining. Identify capsule stain versus other staining procedures.
Pretty fucking difficult to find. Don't mistake crystals for bacteria capsule
34
Don't forget to ________ and ______ the genus
underline and capitalize
35
Capsule staining. What is the secondary stain?
Carbol fuchsin
36
Capsule staining. What was the genus of the organism that was used in class for this stain?
Klebsiella
37
Identify this type of stain
Simple stain
38
Identify this type of staining
Gram staining
39
Identify this type of stain (hint: clumps of bright pink???)
Acid fast
40
Identify this type of stain
Capsule stain
41
Identify this type of stain
Spore staining
42
Identify the organism
_Planaria_ (digestive system)
43
Identify the organism
Tapeworm (scolex)
44
Identify the organism
_Rhizopus_ sporangia zygotes
45
Identify the organism
Hydra
46
Identify the organism
_Rhizopus_
47
Identify the organism
_Penicillium_
48
Identify the organism
_Aspergillus_
49
Identify the organism
_Aspergillus cleistothecia_
50
Identify the organism
Mixed budding yeast
51
Identify the organism
_Diatom strew_
52
Identify the organism
Lichen Thallus
53
Identify the organism
Bacteria capsules
54
Identify the organism
_Bacillus megaterium_
55
Identify the organism
_Streptococcus pyogenes_
56
Identify the organism
_Neisseria gonorrheoea_
57
Identify the organism
Spiral or comma shaped bacteria
58

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