Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MicroLAB > Lab Exam #1 > Flashcards

Lab Exam #1 Flashcards

1
Q

Spore staining. What is the primary stain?

A

Malachite green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Spore staining. What is the mordant?

A

Steam

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Spore staining. What is the decolorizer?

A

distilled water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Spore staining. What is the counterstain?

A

Safranin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Spore staining. What are the two genera common spore formers?

A

Bacillus and Clostridium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Spore staining. What color are endospores at the end of this stain?

A

green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Spore staining. What appears to be pink at the end of this stain?

A

vegetative cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Gram staining. Primary stain?

A

Crystal violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Gram staining. What is the mordant?

A

Gram’s iodine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Gram staining. What is the decolorizer?

A

Acid alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Gram staining. What is the counterstain?

A

Safranin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Gram staining. Which of the chemicals used in the staining procedure that we did in lab does not match the book? (Read the bottle labels in your lab)

A

Acid-alcohol and carbol fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Gram staining. What color is a gram positive cell at the end of the stain?

A

Purple (staphylococcus)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Gram staining. What color is a gram negative cell at the end of the stain?

A

red (Escherichia)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Gram staining. List the shapes and colors of the three genera we used for gram staining

A
  1. Escherichia coli, pink rods 2. Staphylococcus. Purple spheres 3. Bacillus badius. Pink-red rods
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Gram staining. Identify Gram stain versus other staining procedure

A

Gram stain will have purple spheres (crystal violet) whereas your acid fast stain will have blue spheres(methylane blue)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Acid-fast staining. What is the primary stain?

A

Carbol fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Acid-fast staining. For the primary stain, what is the new name when basic fuchsin is mixed with phenol and other non polar solvents?

A

Carbol fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Acid-fast staining. Which chemical in the primary stain acts as the mordant?

A

Phenol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Acid-fast staining. What is the decolorizer?

A

acid alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Acid-fast staining. What is the counterstain? How long did we need to counterstain for to ensure that the contrasting color was visible?

A

Methylene blue;

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Acid-fast staining. What color are acid-fast positive cells at the end of the stain?

A

pink-red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Acid-fast staining. What color are the acid-fast negative cells at the end of the stain?

A

blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Acid-fast staining. What is the genus of the acid-fast organism used in lab?

A

Mycobacterium

25
Q

Acid-fast staining. What is the genus of the organism that was used as a negative control?

A

Staphylococcus

26
Q

Acid-fast staining. Identify acid-fast staining versus other staining procedures

A

Slide will be clumps of pink rods and blue-purple spheres

27
Q

Capsule staining. How is the basis of a negative stain different from the other staining protocols that we performed so far in lab?

A

We are staining the background of the slide instead of the organism

28
Q

Capsule staining. How is the fixing step for the capsule stain different from the other staining protocols that we performed so far in lab?

A

Instead of fixing it with heat, we are fixing it with acid alcohol

29
Q

Capsule staining. What is the primary stain?

A

Congo red

30
Q

Capsule staining. What is the purpose of the acid-alcohol for the capsule stain? (How does this compare to the use of acid-alcohol for the acid-fast stain?)

A

The acid alcohol chemically fixes the slide. In acid fast staining, acid alcohol is used as a decolorizer.

31
Q

Capsule staining. How do capsules appear at the end of the staining procedure?

A

They appear clear with a rod-shaped bacteria in the capsule that can appear red-pink in color

32
Q

Capsule staining. What color is the background?

A

gray, purple, or red

33
Q

Capsule staining. Identify capsule stain versus other staining procedures.

A

Pretty fucking difficult to find. Don’t mistake crystals for bacteria capsule

34
Q

Don’t forget to ________ and ______ the genus

A

underline and capitalize

35
Q

Capsule staining. What is the secondary stain?

A

Carbol fuchsin

36
Q

Capsule staining. What was the genus of the organism that was used in class for this stain?

A

Klebsiella

37
Q

Identify this type of stain

A

Simple stain

38
Q

Identify this type of staining

A

Gram staining

39
Q

Identify this type of stain (hint: clumps of bright pink???)

A

Acid fast

40
Q

Identify this type of stain

A

Capsule stain

41
Q

Identify this type of stain

A

Spore staining

42
Q

Identify the organism

A

Planaria (digestive system)

43
Q

Identify the organism

A

Tapeworm (scolex)

44
Q

Identify the organism

A

Rhizopus sporangia zygotes

45
Q

Identify the organism

A

Hydra

46
Q

Identify the organism

A

Rhizopus

47
Q

Identify the organism

A

Penicillium

48
Q

Identify the organism

A

Aspergillus

49
Q

Identify the organism

A

Aspergillus cleistothecia

50
Q

Identify the organism

A

Mixed budding yeast

51
Q

Identify the organism

A

Diatom strew

52
Q

Identify the organism

A

Lichen Thallus

53
Q

Identify the organism

A

Bacteria capsules

54
Q

Identify the organism

A

Bacillus megaterium

55
Q

Identify the organism

A

Streptococcus pyogenes

56
Q

Identify the organism

A

Neisseria gonorrheoea

57
Q

Identify the organism

A

Spiral or comma shaped bacteria

58
Q
A

MicroLAB (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions