Lab Exam Flashcards by Sarah Midbrød

Urease test /Christensen medium:

urease (+): purple (proteus, klebsiella, helicobacter)

urease (-): citric yellow

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clauberg + löeffler medium?

corynebacterium diphteriae

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Löwenstein-jensen medium?

Mycobacterium tuberculosis

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korthof medium?

leptospira

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holman + thioglycolate media?

clostridium tetani and clostridium perferingens

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wasserman test (CF test)?

syphilis /Treponema Pallidum

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gruber-widal test?

salmonella

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weil-felix test?

rickettsia (also proteus OX19)

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wright test?

brucella/francisella

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elek test?

corynebacterium diptheriae

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Neisser staining?

corynebacterium diptheriae

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Silver impregnation?

leptospira

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ziehl-neelsen staining?

Mycobacterium tuberculosis

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giemsa staining?

plasmodium falciparum and trypanosoma brucei

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coagulase test

coagulase (+): staph. aureus

coagulase (-): other staphylococci

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catalase test?

catalase (+): staphylococci

catalase (-): streptococci

oxidase test?

oxidase (+): pseudomonas, vibrio, campylobacter, neisseria (obligate anaerobes, positive test =pink purple color)
oxidase (-):

indol test (ureum-indol tube):

(+): E. coli (intense red ring on top

(-): Klebsiella

EMB test? (Mackony agar?)

lactose (+): fermenters= E.coli, Klebsiella

lactose (-): salmonella and shigella, proteus

slide agglutination with E coli?

(+): white particles

(-):

TCBS?

Vibrio cholerae

Bismuth sulphite medium?

salmonella

Brilliant green medium?

e.coli and salmonella

DC medium?

shigella and e. coli

Russel medium:

glucose, saccharose and lactose fermenter test (+): deep red (e.coli) (gas production) (-): other sugars than lactose are fermented =little red color and no gas - shigella