Lab Final 2

Question 1

Confirmed Test

Answer 1

performed to confirm if any organism is gram negative and produces acid from latose fermentation

Question 2

Streptococci

Answer 2

Gram +, mostly pathogens, hemolysis test

Question 3

Alpha

Answer 3

incomplete lysis, green zone

Question 4

Beta

Answer 4

clearing around colonies due to complete lysis

Question 5

Gamma

Answer 5

non hemolytic

Question 6

Staphlyococci Media 110 (SM110)

Answer 6

selective due to high salt conc of 7.5%. clear medium.

Question 7

Mannitol Salts Agar (MSA)

Answer 7

selective due to high salt conc of 7.5%. differential for mannitol fermentation due to phenol red pH indicator. fermenters turn plate YELLOW due to acid production

Question 8

Rabbit Plasma

Answer 8

differential for coagulase production. indicated by medium solidifying after inoculation.

Question 9

electroporation transformation

Answer 9

electrical pulses make the cell membrane permeable to allow DNA to enter the cell

Question 10

chemical transformation

Answer 10

treat cells with high concentration of MgSo4 and heat shock

Question 11

storage buffer: polyethylene glycol

Answer 11

acts like antifreeze to keep liquid from freezing

Question 12

storage buffer: glycerol

Answer 12

helps reduce ice crystal formation

Question 13

divalent cations (MgCl2 or CaCl2)

Answer 13

neutralize charge of cell wall

Question 14

Experimental plate

Answer 14

LB-AMP plate spread with cells with plasmic added. expect growth of isolated colonies

Question 15

Positive Control

Answer 15

LB Plate with cells and water. expect lawn. tests for ability to survive transformation

Question 16

Negative Control

Answer 16

LB-AMP plate with cells and water. expect 0 growth. tests for previous antibiotic resistance

Question 17

Plasmid Inhibition Control

Answer 17

LB medium with cells and plasmic. Indicated if cells survived transformation and that the addition of pUC18 did not inhibit growth. expect lawn.

Question 18

resazurin

Answer 18

indicates if oxygen is present (pink) or not (clear)

Question 19

sodium thioglycolate

Answer 19

reduces free oxygen in the medium to water

Question 20

fluid thioglycolate medium

Answer 20

broth that allows for growth of anaerobes in aerobic conditions

Question 21

brewer’s agar

Answer 21

used to streak for growth in anaerobic jar

Question 22

anaerobic jar

Answer 22

sealed incubation container

Question 23

gaspak

Answer 23

added to jar to create anaerobic environment. generates H that binds with O2 present to reduce it to H2O

Question 24

methylene blue strip

Answer 24

indicates if oxygen is present (blue) or not (white)

Question 25

Kirby Bauer Assay

Answer 25

standard method to determine sensitivity of an organism to antibiotics

Question 26

Mueller Hinton Medium

Answer 26

antibiotic assay, clearer drug diffusion

Question 27

antiseptic

Answer 27

kill or inhibit microorgs on a person's skin

Question 28

disinfectant

Answer 28

kill or inhibit microorgs on surfaces

Question 29

bacteriostatic

Answer 29

prevent or inhibit growth but do not kill bacteria

Question 30

bactericidal

Answer 30

capable of destroying bacteria

Question 31

germicidal

Answer 31

kill or inactivate microbes

Question 32

coliforms

Answer 32

non endospore forming, gram neg, rods, facultative anaerobes that ferment lactose to produce gas + acid within 24 hours at 37 degrees C

Question 33

satellite colonies

Answer 33

The formation of satellite colonies are caused by the degradation of ampicillin to lower levels which is done by the colonies who have become resistant to the antibiotic