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Microbiology > Lab Practical 1 > Flashcards

Lab Practical 1 Flashcards

1
Q

Why is it necessary to heat fix bacterial cells

A

to get bacteria to stick to the glass

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2
Q

a common stain that is used in simple staining

A

crystal violet

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3
Q

give two reasons for cooling the slide before applying the stain

A

prevent cracking and prevent participate

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4
Q

the stain used in a Negative stain is

A

Nigrosin

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5
Q

Why is Nigrosin called the negative stain

A

because it makes ligh structures dark and dark structures light

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6
Q

the easiest staining procedure is

A

a simple stane

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7
Q

why is the simple stain valuable

A

identify organisms/inhibit growth

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8
Q

the type of mount that you use in the lab to study organisms for a short period of time is

A

temporary mount

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9
Q

why should you flame the inoculation loop while using it

A

to sterilize it

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10
Q

the microscope used in the labratory is a

A

compound binocular light microscope

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11
Q

the ability of a microscope to reveal fine detail is known as

A

greater resolution

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12
Q

the 10X eyepiece used in combination with a 45X objective produces a magnification of

A

450X

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13
Q

another name for the 100X objective lens is

A

oil immersion objective

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14
Q

when putting the microscope away which objecive should be in place

A

low power objective

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15
Q

if the letter e is moved to the right, in which direction does it appear to move in the field of view

A

left

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16
Q

what part of the microscope regulates the amount of light enering

A

the condenser

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17
Q

what part of the microscope is used if some focusing adjustment is needed with high power

A

find adjustment knobs

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18
Q

identify the person who was first to observe microorganisms

A

van leewenhoek

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19
Q

which of the folowing cannot be seen with your labratory microscope

A

viruses

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20
Q

the primary stain in an acid fast stain is

A

carbolfuschin

21
Q

the counterstain is

A

methelyne blue

22
Q

an example of an acid fast positive organism is

A

Mycobacterium

23
Q

acid fast positive organism stian what color

A

red

24
Q

the decolorizer used in an acid fast stain is

A

95% acid alcohol

25
Q

what enables the acid fast bacteria to retain the primary stain

A

heating

26
Q

acid fast negative organisms stain what color

A

blue/green

27
Q

a colony with a form such as O is described as

A

cocci

28
Q

when idenfying organisms according to growth what precaution should be taken after inocculating the tubes

A

shaking tube

29
Q

can you tell celllular contents or cell shape from cellular morphology?

A

cell shape

30
Q

a culture in which you have many diifferent types are associated togehter is considere to be

A

mixed

31
Q

the single most important bacteriological stain is

A

gram stain

32
Q

the counter stain used in a gram stain is

A

safranin

33
Q

idetify the decolorizing agent in a gram stain

A

95% ethyl alcohol

34
Q

gram negative bacteria stain what color

A

red

35
Q

gram positive bacteria stain what color

A

purple

36
Q

the most critical part of the gram staining procedure is

A

95% ethyl alchohol

37
Q

what techinique used to maintain sterility and avoid contamination is called

A

aseptic technique

38
Q

turbidity can best be seen on what time of plate

A

broth

39
Q

what is the purpose of flaming your loop

A

to sterilize

40
Q

the primary stain used in the endospore procedure is

A

malchite green

41
Q

what color do the endospores stain

A

green

42
Q

why do you steam the endospore stain

A

to put cracks in the spore wall to allow dye in

43
Q

another name for the endospore stainis

A

shaeffer-fulton method

44
Q

what counter stain is used in an endospore stain

A

safranin

45
Q

two genera most noted for production of endospores

A

bacillis and clostridium

46
Q

an endospore is a vegetative or dormant cell

A

dormant cell

47
Q

the color produced which indicates an organism uses citrate as a carbon source

A

blue

48
Q

E. Coli is citrate positive or negative

A

negative

49
Q

the steps of a gram stain

A
  1. apply crystal violet for 30 seconds
  2. add iodine grams for 30 seconds
  3. rinse with 95% ethyl alcohol until majority of stain runs off (20 seconds)
  4. ) counterstain with safranin for 30 seconds

Microbiology (4 decks)

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