Lab Quiz 1

Question 1

What are the required materials for lab?

Answer 1

Safety goggles with side shield, lab handout, close-toed-shoes, and internet device

Question 2

When you enter and leave the room, what should you do?

Answer 2

Wipe table and wash hands

Question 3

What do you need to wear for lab?

Answer 3

Safety goggles, close-toed shoes, legs covered

Question 4

Where should you store food/drinks/backpacks?

Answer 4

At front of the classroom or in the cabinet

Question 5

If an injury occurs, what should you do?

Answer 5

Notify instructor

Question 6

If there is a spill or broken equipment, what should you do?

Answer 6

Notify instructor

Question 7

Small chemical spill: what should you do?

Answer 7

napkins

Question 8

Large chemical spill: what should you do?

Answer 8

Hazchem spill response kit, chemical shower

Question 9

Emergency evacuation: what is the first thing you should do?

Answer 9

Secure station if time permits

Question 10

Emergency evacuation: what are the steps?

Answer 10

Secure station if time permits, walk to designated exit and down stairwell, meet in lot 2. Gather at lot 2, then notify instructor of presence. Wait for signal for re-entry

Question 11

What goes down the drain?

Answer 11

Nothing, except for water you wash your hands with

Question 12

What goes in the trash?

Answer 12

Nothing, except for napkins you used to wash your hands with

Question 13

Where do gloves go?

Answer 13

Burn box

Question 14

What goes in the blue recycling bin?

Answer 14

Paper, aluminum foil, cardboard

Question 15

What goes in the glass recycling bin?

Answer 15

Not broken glass

Question 16

What goes into the glass recycling BUCKET?

Answer 16

Broken glass

Question 17

What goes in the burn box?

Answer 17

Pipettes and gloves

Question 18

What goes into the biohazardous waste bin?

Answer 18

bacterial cultures, used pipette tips with certain bacteria on them

Question 19

Where do you dispose of liquid chemicals?

Answer 19

In the hood, into a waste jar

Question 20

Blue part on the NFPA

Answer 20

Health Hazard:
4= can be lethal,
3= can cause serious injury,
2= can cause temporary incapacitation or residual injury,
1= can cause significant irritation
0=no hazard

Question 21

Red part on the NFPA

Answer 21

Flammability Hazard:
4= will vaporize and burn at normal temperatures.
3= can be ignited at almost all ambient temperatures
2= must be heated or high ambient temperature to burn.
1= must be preheated before ignition can occur
0= will not burn

Question 22

White part on the NFPA

Answer 22

Special hazard:
OX= oxidizing.
SA = simple asphyxiants
W crossed out = reacts violently or explosively with water

Question 23

Yellow part on the NFPA

Answer 23

Instability hazard:
4= may explode at normal temperatures and pressures.
3= may explode at high temperature or shock
2 = violent chemical change at high temperatures or pressures
1= normally stable. high temperatures make unstable.
0= stable

Question 24

base units of measurement

Answer 24

meter, liter, gram

Question 25

When should you use a micropipette

Answer 25

when pipetting less than a mL

Question 26

when should you use a p-1000?

Answer 26

when pipetting 100-1000 microliters

Question 27

p-20

Answer 27

2 microliters to 20 microliters

Question 28

how to set volume on a micropipette

Answer 28

gently pull up and turn black wheel, then push black wheel back into original position

Question 29

before pipetting anything:

Answer 29

place a disposable plastic pipette tip

Question 30

how to hold a micropipette

Answer 30

place tip onto micropipette by holding micropipette by handle and then putting the pipette tip-down, sliding it into the tip.

Question 31

what does the plunger do?

Answer 31

fluid taken up into and expelled from the pipette tip by using the micropipette; push down on the button

Question 32

plunger stops: how many

Answer 32

2 stops

Question 33

how do you aspirate fluid into the pipette tip?

Answer 33

push the plunger button down to the first stop. continue to hold it as you place the pipette tip into a fluid you want. Only very end of pipette tip needs to be placed into fluid. Release button slowly to draw fluid into pipette. Keep pipette in the fluid until the plunger has completely regained original position. Draw tip out of the fluid into the microcentrifuge tube.

Question 34

how do you expel fluid from the pipette tip?

Answer 34

place pipette into tube where you want to expel liquid, slowly push down on plunger from first stop to the second stop. Hold down button until pipette is no longer touching the fluid

Question 35

what direction should you hold the pipette?

Answer 35

straight down

Question 36

what speed should you let the plunger up?

Answer 36

slowly

Question 37

relationship between transmittance and absorbance

Answer 37

absorbance = -log transmittance

Question 38

when to use a P10

Answer 38

.5 microliters-10 microliter

Question 39

where should you dispose of needles or razors?

Answer 39

Sharp disposal box (small and red), or orange and white burn-up bin

Question 40

Where should you dispose of non-sharp biohazards?

Answer 40

Large, red bins with plastic biohazard bag inserts