Lab Quiz 4 Flashcards
(17 cards)
Define General Purpose and give an example
Contains all necessary nutrients to grow a wide variety of microorganisms
EX: TSA
Define selective media and give an example
Certain specific components that allow for growth of certain microorganisms while restricting the growth of certain microorganisms while restricting the growth of others
EX: MSA,MAC,C-CNA
Define differential media and give an example
Contain components that allow for a discernible between microorganisms within a specific group based on visual appearance
EX: MSA,MAC,BLOOD,C-CNA
Define enriched media and give an example
Contains specific growth factors needed by fastidious bacteria to support their growth
EX: BLOOD AGAR
What does the term “fastidious” mean and what type of media do fastidious organisms require?
An organism that will not grow without specific factors present or in a specific condition.
Enriched media
What does the term capnophile mean and how did we cultivate capnophilic bacteria in lab?
Microorganisms that thrive in the presence of high concentrations of carbon dioxide.
Place into a anaeropack box to incubate in an environment enriched with carbon dioxide to encourage the growth of capnophiles.
PH indicators are an example of which type of media component?
Differential
What typically happens to the pH of a medium after bacteria have metabolized carbohydrates (glucose, lactose, sucrose, maltose, etc.)
Decreases
What typically happens to the pH of a medium after bacteria have metabolized proteins/amino acids?
Increases
For each of the following pH indicators,state how each would appear in NEUTRAL, ACIDIC, AND ALKALINE pH (if applicable)
-PHENOL RED
-NEUTRAL RED
-EOSIN METHYLENE BLUE
Phenol Red
Acidic-yellow Neutral-Red Basic-purple/hot pink
Neutral Red
Acidic-pink Neutral-Maroon Basic-N/A
Eosin Methylene Blue
Acidic-metallic green/pink Neutral-dark maroon Basic-N/A
MSA
Full name
State whether SELECTIVE (S), DIFFERENTIAL (D), ENRICHED (E)
Selective component(s) and what type of organisms it selects for (if applicable)
Differential component(s) (if applicable)
Enrichment component(s) (if applicable)
Mannitol salt agar
Selective & differential
7.5 % NaCl halotolerant
Mannitol & phenol red
N/A
Blood Agar
Full name
State whether SELECTIVE (S), DIFFERENTIAL (D), ENRICHED (E)
Selective component(s) and what type of organisms it selects for (if applicable)
Differential component(s) (if applicable)
Enrichment component(s) (if applicable)
N/A
Differential/enriched
N/A
Sheep’s blood
Sheep’s blood
MAC
Full name
State whether SELECTIVE (S), DIFFERENTIAL (D), ENRICHED (E)
Selective component(s) and what type of organisms it selects for (if applicable)
Differential component(s) (if applicable)
Enrichment component(s) (if applicable)
MacConkey agar
Selective/differential
Crystal violet & bile salts (gram positive)
Lactose & neutral red pH indicator
N/A
EMB Agar
Full name
State whether SELECTIVE (S), DIFFERENTIAL (D), ENRICHED (E)
Selective component(s) and what type of organisms it selects for (if applicable)
Differential component(s) (if applicable)
Enrichment component(s) (if applicable)
Eosin & Methylene Blue Agar
Selective/differential
Eosin & methylene Blue (gram negative)
Lactose
N/A
CNA
Full name
State whether SELECTIVE (S), DIFFERENTIAL (D), ENRICHED (E)
Selective component(s) and what type of organisms it selects for (if applicable)
Differential component(s) (if applicable)
Enrichment component(s) (if applicable)`
Colistine nalidixic acid agar
Selective/differential/enriched
Antibiotics (gram positive)
Sheep’s blood
Sheep’s blood
TSA
Full name
State whether SELECTIVE (S), DIFFERENTIAL (D), ENRICHED (E)
Selective component(s) and what type of organisms it selects for (if applicable)
Differential component(s) (if applicable)
Enrichment component(s) (if applicable)`
Triple sugar iron agar test
Differential
N/A
Phenol red pH indicator, thiosulfate
N/A
Know the details of each type of media we used in lab:
Know what can be determined by the use of each type of media
Be able to interpret results for each medium
