lab quiz # 4 Flashcards
(22 cards)
IMVIC
Indole
Measures protein metabolism, ability of organism to convert tyrptophan to amino acid
Media-TRYPTONE BROTH
Detection reagent- KOVAC’S
Pos=RED
Neg= YELLOW
IMVIC
METHYL RED
Carbohydrate fermentation
Media-MR-VP BROTH
Detection reagent-METHYL RED
Pos=RED
Neg=YELLOW
IMVIC
VOGES-PROSKAUR
pos result=organism has produced acetoin through butanediol fermentation pathway
Media-MR-VP BROTH
Detection Reagents- BARRET’S
Pos= RED Neg= YELLOW
IMVIC
CITRATE
Measures organisms ability to grow using only citrate (organic acid) carbon source
Media- CITRATE SLANT
Detection Reagent- BROMTHYMOL BLUE
Pos= BLUE Neg= GREEN
IMVIC CONTROLS
E. COLI= (+ )(+)(+)( -)
ENTERO. aerogenes= (-)( -)( +)( +)
PHENYLALANINE DEAMINASE
AMINO ACID
POS RESULT= ORGANISM PRODUCED DEAMINASE ENZYMES WHICH REMOVE AN AMINO GROUP
MEDIA USED= PHENYLALANINE AGAR
INDICATOR= ACIDIFIED FERRIC CHLORIDE
POS= GREEN NEG= YELLOW
MORGENELLA MORGANII (+) E. COLI (-)
DNASE
DETECT DNASE ACTIVITY IN STAPYLCOCCUS ORGANISMS
INDICATOR= DNASE AGAR/ W/ METHYL GREEN
STAPHYLOCOCCUS AUREUS SHOWED VERY STRONG POSITIVE RESULTS
STAPH. EPIDERMIDIS (-)
STAPH. AUREUS (++)
STAPH SAPROPHYTICUS (+)
NITRATE REDUCTION
AFTER AN ORGANISM WAS GROWN IN NITRATE BROTH FOR 48 HRS, AND TURNED RED AFTER THE ADDITION OF REAGENTS THIS SHOWS THAT IS IS POSITIVE FOR NITRATE REDUCTASE
THE RESULT WOULD BE NEGATIVE FOR BOTH NITRATE AND NITRITE REDUCTASE BECAUSE AFTER ADDING ZINC THERE WAS A COLOR CHANGE
THE RESULT WOULD BE POSITIVE FOR BOTH NITRATE AND NITRITE BECAUSE EVEN AFTER THE ADDITION OF ZINC THE ORGANISM REMAINED COLORLESS
ENTEROTUBE
THE GRAM (-) ROD MUST BE OXIDASE NEGATIVE
OXI/FERM TUBE
THE GRAM (-) ROD MUST BE OXIDASE POSITIVE
COAGULASE TEST
DETECTS THE ABILITY OF AN ORGANISM TO COAGULATE PLASMA
MEDIA= STERILE RABBIT PLASMA
POS= SOLID
STAPH. AUREUS (+)
STAPH. EPIDERMIS (-)
Virulence factor=the degree to which a microbe is pathogenic.
being able to coagulate provides a protective barrier against phagocytic cells
RAPID PLASMA REAGIN (RPR)
DETECTS THE PRESENCE OF REGAIN (ANITBODY)
SEROLOGICAL TEST FOR SCREENING FOR SYPHILIS. IT DETECTS THE PRESENCE OF REAGIN, A NONSPECIFIC AB PRESENT IN THE BLOOD OF PEOPLE WITH SYPHILIS
INDICATOR= ANITGEN COATED RED BEADS
POS= AGGLUTINATE THE COLORED BEADS. NEG= CLOUDY
TREPONEMA PALLIDUM CAUSES SYPHILIS.
SINCE THIS TEST DETECTS NONSPECIFIC AB FALSE NEGATIVES CAN OCCUR
BACTERIAL AGGLUTINATION
TEST UTILIZES SPECIFIC AB-AG BINDING TO IDENTIFY A SPECIFIC BACTERIA
positive result= clumping
E. coli (-)
Salmonella (+)
DIRECT AGGLUTINATION
ARE MIXED WITH BACTERIA AND IF THE AB BIND TO THE PROTEINS ON THE SURFACE OF THE BACTERIA VISIBLE CLUMPS WILL FORM
INDIRECT AGGLUTINATION
COLORED LATEX BEADS ARE COVERED WITH AG AND MIXED WITH AB (MUCH EASIER TEST)
YEAST FUNGI
SACHCAROMYCES CEREVISIAE IS USED TO FERMENT SUGARS FOUND IN GRAPES BECAUSE IT PRODUCES ALCOHOL AS A BYPRODUCT
SABOURAND DEXTROSE AGAR PLATES (SAB) SELECTIVE FOR THE GROWTH OF FUNGI OVER BACTERIA (LOWER PH)
SACHCAROMYCES (GRAM +) WHITE OPAGUE
(BUBBLES AND ALCOHOL)
RHODOTORULA RUBRA (GRAM -) PINK OPAGUE (NO BUBBLES OR ALCOHOL)
GERM TUBE TEST
TO IDENTIFY CANDIDA ALBICANS
GERM TUBES ARE ELONGATED PSEUDOHYPHAE PRODUCED ONLY BY CANDIDA.
SACCHAROMYCES (NO GERM TUBE)
FILAMENTOUS FUNGI
RHIZOPUS- GREY WITH BLACK EDGES =SPORANGIOSPORE (SAC)
PENICILLIUM- GREEN CENTER SURROUNDED BY WHITE
=CONIDIOSPORE (NO SAC) ASEXUAL
ASPERGILLUS -BLACK
=CONIDIOSPORE
LIVE PROTOZOA
AMOEBA = PSEUDOPODIA EUGLENA= FLAGELLA PARAMACIUM= CILIATE VORTICELLA= CILIATE STENTOR = FLAGELLA
HYDOGEN SULFIDE PRODUCTION
TSI=TRIPLE SUGAR AGAR FOR CONTROLS
(WE USED PEPTONE IRON AGAR)
INDICATOR PHENOL RED
A/A=GLUCOSE AND LACOTSE &/OR SUROSE WERE FERMENTED
A/A+H2S=2 OR MORE SUGARS WERE FERMENTED AND GAS PRODUCED; BLACK IS PRESENT
K/A=ONLY GLUCOSE WAS FEREMENTED(K=ALKALINE REACTION/RED &A=ACID/YELLOW)
K/K= NO SUGARS WERE FERMENTED
K/A +H2S=GLUCOSE WAS FERMENTED AND BLACK PPT IS PRESENT
A/A= 2 OR MORE SUGARS WERE FERMENTED AND GAS PRODUCED (NO H2S PRODUCED)
E. COLI= (A/A) SALMONELLA TYPHIMURIUM (K/A+H2S) CITROBACTER FREUNDII (A/A + H2S) PROVIDENCIA ALCALIFACIANS (K/A)
EFFECTS OF DISINFECTANTS AND ANTISEPTICS
MOST EFFECTIVE LEASE EFFECTIVE
E. COLI BLEACH PURELL
S. AUREUS BLEACH PURELL
EFFECTS OF ANTIBIOTICS
MEDIUM: NA AGAR
MOST EFFECTIVE LEAST EFFECTIVE E. COLI OFLAXACIN PENICILIN/ERYTHO
S. AUREUS PENICILIN TRIMETHOPRIM
OLFAXACIN HAD EQUAL EFFECT ON BOTH
MIC= MG/ML
