Lab Safety

Question 1

When do you disinfect your lab area?

Answer 1

before and after each lab session

Question 2

How do you disinfect the lab work area?

Answer 2

wet the counter with disinfectant and wipe over surface with paper towel

Question 3

When should you wash your hands?

Answer 3

before leaving lab

Question 4

How long should you wash your hands?

Answer 4

for at least 15 seconds

with soap and water

Question 5

How do you avoid inadvertent contamination to your mouth?

Answer 5

keep fingers and pencils away from it

Question 6

Can you eat and drink in the lab?

Answer 6

no

Question 7

What do you do if equipment is missing or not working properly (especially microscopes)?

Answer 7

report to the instructor immediately

Question 8

Are you allowed personal effects in the lab?

Answer 8

no

Question 9

What is the lab coat policy?

Answer 9

must wear a buttoned lab coat at all times

Question 10

How do you clean your lab coat between labs?

Answer 10

• take home in plastic bag, do not let come into contact with other belongings
• wash separately from other clothes with detergent and bleach

Question 11

Can your lab coat be worn in other labs before it is washed?

Answer 11

no, it must be washed first

Question 12

What do you do if you know/suspect your lab coat has been contaminated with bacterial culture?

Answer 12

report incident to demonstrator

Question 13

What happens when the demonstrator determines the appropriate means of decontaminations for a contaminated lab coat?

Answer 13

spot disinfection or sterilization in the autoclave

Question 14

What happens if sterilization int he autoclave is needed for a contaminated lab coat?

Answer 14

lab coat remains overnight in the lab and returned to you the following day

Question 15

What does risk group 1 cultures mean?

Answer 15

unlikely to cause disease in healthy workers

Question 16

What does risk group 2 cultures mean?

Answer 16

can cause human disease but are unlikely to under normal circumstances (immune compromised may be at risk)

Question 17

How should you treat all organisms in the lab?

Answer 17

like they cause disease

Question 18

What are the safety measures when handling risk group 2 organisms?

Answer 18

• wear disposable gloves

- be aware of aerosols when flaming loops

Question 19

Where is paper waste discarded?

Answer 19

regular waste paper baskets

Question 20

Where is used petri plates discarded?

Answer 20

large biohazard bins sitting beside door

Question 21

Where is disposable gloves discarded?

Answer 21

large biohazard bins sitting beside door

Question 22

Where is glass slides, broken glass and Pasteur pipettes discarded?

Answer 22

broken glass/sharps discard containers

Question 23

Where is used swabs and toothpicks discarded?

Answer 23

broken glass/sharps containers

Question 24

Where is disposable pipettes discarded?

Answer 24

broken glass/sharps containers

Question 25

What do you do with used glassware (test tubes, beakers, bottles and flasks)?

Answer 25

- clean of all labels | - place upright in the trays on the glassware discard trolleys

Question 26

Do you pour stuff down the sink in the MBIO lab?

Answer 26

NO

Question 27

What do you do for a spilled bacterial culture when broken glass is involved?

Answer 27

notify instructor or demonstrator immediately - do not pick up glass with fingers - prevent other students from walking in it - instructor cleans up

Question 28

What do you do for a spilled bacterial culture if no broken glass is involved? (5 steps)

Answer 28

- cover spill with paper towel - squeeze disinfectant onto outer edges of spill and work towards centre - allow to sit for at least 1 minute - gather up soaked towels (throw in regular garbage) - disinfect area again with fresh paper towels and discard in regular garbage

Question 29

Why do you squeeze disinfectant onto outer edges of spill and work towards centre on a bacterial culture spill?

Answer 29

minimizes amount of aerosols

Question 30

What do you do if culture is splashed on skin?

Answer 30

wash skin with alcohol (handwashing sink), then wash with soap and water

Question 31

What do you do if culture is splashed into eyes?

Answer 31

rinse eyes with water from nearest tap/eyewash station | -avoid rubbing eyes

Question 32

What do you do for accidental contamination of the mouth?

Answer 32

treated by rinsing with copious amounts of water and reported to the instructor

Question 33

What do you do for cuts or wounds not he hands or fingers?

Answer 33

should be kept covered by bandages, and the bandage should be covered with a glove -see TA if you need a bandaid

Question 34

What do you do for clothes that have been contaminated?

Answer 34

- disinfected with small amount of disinfectant (from washing sinks) and then allow to dry completely - once dried, contaminated clothes should be washed separately with detergent and bleach

Question 35

What do you do if a fire occurs?

Answer 35

turn off gas and call for lab instructor

Question 36

What must be done to hair in the lab?

Answer 36

- long hair tied back | - excessive use of hairspray and other products should be avoided

Question 37

How do you light a Bunsen burner?

Answer 37

with a striker provided

Question 38

What to do when clothing is on fire?

Answer 38

smother it

Question 39

What do you do in case of a fire alarm?

Answer 39

wait for signal from instructor to evacuate lab, head for stairs and exit

Question 40

Do you use the pilot flame of the Bunsen burner for sterilization?

Answer 40

NO

Question 41

What does WHMIS stand for?

Answer 41

Workplace Hazardous Materials Information System

Question 42

What is WHMIS?

Answer 42

a system for safe management of hazardous materials

Question 43

Who legislates WHMIS?

Answer 43

provincial and federal governments

Question 44

What are the 3 parts of WHMIS?

Answer 44

cautionary labels, safety dada sheets (SDS) and worker education programs

Question 45

What does SDS stand for?

Answer 45

safety data sheets

Question 46

What are cautionary labels?

Answer 46

placed on all containers with controlled products

Question 47

What 2 places must cautionary labels be placed on?

Answer 47

containers obtained by suppliers and those dispensed in the workplace

Question 48

What 7 parts must be included on the supplier labels?

Answer 48

- product identifier - supplier identifier - SDS - hazard symbol - risk phrases (nature of hazards) - precautionary measures - first aid measures

Question 49

What are the 3 parts of workplace labels?

Answer 49

- product name - precautionary measures (simplified) - reference to availability of SDS

Question 50

What does MSDS stand for?

Answer 50

material safety data sheets

Question 51

What is MSDS

Answer 51

links to online SDS for controlled products and biohazardous materials available on installed laboratory computers