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LAB SAFETY AND QUALITY CONTROL MEASURES Flashcards

(67 cards)

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1
Q

Hazard identification
Risk assessment and control
Biosafety and biosecurity measures
Safety skills, knowledge and training

A

Elements of laboratory safety

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2
Q

Use a Biosafety Level 2 (BSL2) facility
Separate laboratory for fungal and viral soecimens
Use a Class II biosafety cabinet
Ensure proper monitoring and maintenance

A

Fungal Specimen processing

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3
Q

TRUE OR FALSE

Fungal infection and contamination is more complicated to handle than bacterial agents

A

TRUE

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4
Q

TRUE OR FALSE

Do not always wear PPE during specimen processing

A

FALSE

always wear PPE during specimen processing

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5
Q

When handling clinical isolates especially dimorphic pathogens what position do we maintain it so that it prevents aerosolization

A

Maintain on slants

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6
Q

Plated media Handling

Seal with ___ before removing from the biosafety cabinet

A

gas-permeable tape

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7
Q

Culture vessels for fungal culture must be sealed because of ___

A

spores

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8
Q

Surface decontamination

Use __% bleach for surfaces in contact with clinical specimens or culture

A

10%

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9
Q

Dispose of clinical materials

Use ___ bags secured with tape and dispose in designated biomedical waste containers

A

biohazard

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10
Q

Personne in the Mycology laboratory should be

A

Healthy
Not on antibiotics or immunosuppresive drugs
Trained on safety techniques

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11
Q

Biological Safety Cabinet (BSC)
Alcoholic sand flask
Plastic-baked gauze/cotton pads
Stainless steel containers

A

Essential equipment and materials

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12
Q

Mycology laboratory

Prohibited activities

A

smoking, eating

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13
Q

Mycology laboratory

Handwashing protocols

A

Soap and water; disinfectant solution

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14
Q

Mycology laboratory

Use of biological safety cabinets

A

BSC Class IIA

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15
Q

Mycology laboratory

Transport of cultures and specimens

A

racks or trays

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16
Q

Mycology laboratory

Aerosol reductions

A

electric incinerators minimize aerosol and heat output

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17
Q

Mycology laboratory

Handling contaminated liquids

A

splash-proof containers; avoid aerosols

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18
Q

BSL 2 work practices and facilities
Use of secondary containers for specimen transport
Specimen processing in Class II biosafety cabinets
Disinfection with bleach and alcohol

A

Virology Laboratory Safety considerations

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19
Q

Boiling
Pasteurization
UV Light irradiation

A

Physical Methods of disinfection

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20
Q

Not less than 62.8°C or more than 65.6°C for at least 30 min (LTLT; holder method)

Not less than 71.7°C for at least 15 s (HTST)

A

Pasteurization

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21
Q

At 100°C for 15 minutes

A

Boiling

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22
Q

Not recommended for disinfection of biosafety cabinets because of ineffective penetration; __ deteriorates plastic in the BSC; ___ light intensity decreases over time

A

UV light irradiation

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23
Q

Ethanol (60%-80%)
Ethanol (70%)
Phenolic compoinds
Quaternary ammonium compounds
Chlorine bleach
Chlorine bleach followed by rinsing with 70% alcohol or water

A

Chemical methods of disinfection

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24
Q

Active against enveloped viruses (Herpesviruses, vaccinia virus, influenza virus) and non-enveloped viruses (adenovirus, enterovirus, rhinovirus and rotaviruses) ; also effective against bacteria, fungi, and mycobacteria

A

Ethanol (60%-80%)

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25
Disinfects external surfaces of equipment and small surfaces
Ethanol (70%)
26
Bactericidal, fungicidal, tuberculocidal, and virucidal but not effective against enterovirus
Phenolic compounds
27
Fungicidal, bactericidal and virucidal (only against enveloped viruses); not sporicidal and tuberculocidal
Quaternary ammonium compounds
28
Recommended for surface disinfection and has a broad spectrum of activity; used for decontamination of blood spills (1:10 dilution of 5.15% to 6.25% sodium hypochlorite)
Chlorine bleach
29
Recommended to decontaminate stainless steel surfaces
Chlorine bleach followed by rinsing with 70% alcohol or water
30
Moist heat (steam) Incineration Flame sterilization Dry heat (hot air)
Physical methods of sterilization
31
Ethylene oxide
Chemical Methods of Sterilization
32
Sterilization of supplies and media (121°C for 15 minuets) and medical wastes (132°C for 30 to 60 minutes)
Moist heat (steam)
33
Medical wastes
Incineration
34
Used for metal tools including inoculating loops and wires, tips of forceps, glass objects such as culture tubes and microscope sludes
Flame sterilization
35
For sterilization of dry glassware including flasks, pipettes, tubes, petri dishes, and metal instruments
Dry heat (hot air)
36
Most common chemical method
Ethylene oxide
37
Recommended when working with microbes not known to cause disease in healthy adults (e.g Saccharomyces cerevisae)
BSL 1
38
Access is restricted to authorized personnel Readily accessible sinks for hand washing Readily accessible eyewash stations Wearing of appropriate PPE Laboratory bench tops must be resistant to chemicals and liquids Laboratory surfaces and equipment are easily cleaned and disinfected Decontamination of solid wastes
BSL 1
39
Recommended for microbes associated with human disease but are not aerosol-transmitted (e.g Candida ablicans, Cryptococcus neoformans, Hepatitis B virus, Herepes simplex virus)
BSL 2
40
Universal biohazard signs Processing of specimens in a BSC Decontamination of equipment must be available (e.g autoclave) Use of appropriate PPE Sharps are placed in a puncture-resistant containers
BSL 2 (+ BSL 1 practices)
41
Recommended for hazardous microbes that are primarily aerosol-transmitted (e.g Blastomyces dermatitidis, West nile virus)
BSL 3
42
Controlled access to the laboratory Cultures and clinical materials are processed in a BSC (Class II) Negative-pressure airflow HEPA-Filtered exhaust air from BSCs Use of appropriate PPE and containment devices HEPA-filtered respirators or masks Serologic determination of immune status of personnel
BSL 3 (+ BSL 1 and 2 practices)
43
Recommended for agents that can cause life-threatening or untreatable diseases brought about by aerosols or unknown transmission (Ebola virus, Marburg virus, Crimean-congo hemorrhagic fever virus)
BSL 4
44
Change of clothing before entering, and shower on exit Decontamination of all wastes of exit Use of BSC (Class III) Specialized ventilation and waste management systems
BSL 4 (+ BSL 3 practices)
45
Goal of Quality Control
Accuracy Reliability Reproducability
46
Closeness to actual results
Accuracy
47
Standard of quality over time
Reliability
48
Consistency of measurement -Different appraisers -Same equipment
Reproducibility
49
Check for cracks, uneven filling, hemolysis, freezing, drying out, bubbles,contamination
Inspection of media and petri dishes
50
Verify media are within expiration dates
Expiration dates
51
Check for contamination or foreign substances
Reagent inspection
52
Record temperatures of refrigerators, freezers, and incubators Acceptable variation:±1°C Include humidity and CO2 readings if applicable
Daily temperature monitoring
53
Avoid placing objects on grid panels to maintain airflow (Perform smoke testing before use)
Biological safety cabinets
54
Use distilled or deionized water Maintain temperature fluctuation within ±1°C
Water baths
55
Follow manufacturer's instructions or package insert strictly
Test kit storage
56
Waste disposal Chemical wastes :Treatment methods
Decomposition, decontamination, landfill disposal
57
Waste disposal Chemical wastes Flammable fluids and chemicals
Original, securely sealed, and labeled containers Never pour down the drain
58
Waste disposal Chemical wastes Risks of improper disposal
Hazardous fumes Disruption of sewage treatment facilities Damage to laboratory plumbing
59
Waste disposal Chemical wastes Disposal of non hazardous solvents : Water soluble and less than a pint
flush with plenty of water
60
Waste disposal Chemical wastes Disposal of non-hazardous solvents : For non-soluble or volumes over one pint
use a designated solvent waste container
61
Waste is labeled, segregated by compatibility, stored in secondary containment, and in an isolated area
Proper hazardous waste storage
62
No VEHS labels No secondary containment No segregation and containers covered in residue
Improper hazardous waste storage
63
Waste disposal Solid wastes Risk of puncture wounds and infectious agents Use leakproof, puncture-proof containers labeled as contaminated waste
Needles, syringe, and scalpels
64
Waste disposal Solid wastes Dispose in labeled cardboxes, not reular trash cans
Broken and damaged glassware
65
Waste disposal Solid wastes Paper towels, packaging materials, certain plastics Discard in regular trash bins
Non-contaminated waste
66
Waste disposal Solid wastes Highest risk level in medical mycology laboratory: includes clinical specimens, contaminated materials, cultures
Biologically contaminated waste
67
Waste disposal Solid wastes Use discard pans with 5% hypochlorite solution or similar disinfectant
Biologically contaminated waste

MYVCOVIRO LAB (2 decks)

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