Lab Safety/Waste Disposal/Hand-washing

Question 1

4 Main Hazards:

1. Biohazard
2. Sharpes
3. Fire
4. Chemicals

Answer 1

Safety Equipment:

1. Eye wash
2. Fire blanket
3. Fire extinguisher:
   
   1. Pull Pin
   2. Aim the nozzle at the base of the fire
   3. Squeeze handle continuously

Question 2

Dress Code:

Long hair contained
Long lab coat w/ long sleeves
No exposed legs
Closed toe shoes
Gloves
No dangling jewelry or sleeves

Answer 2

General Lab Procedures:

1. Wash hands before you begin lab and every time you leave
2. Disinfect lab bench before and after each lab

Question 3

Hand-washing Procedures:

1. Read about hand-washing
2. Have paper towel ready
3. Turn on water
4. Get soap
5. Lather up and rinse while rubbing hands together for 30 seconds
6. Dry hands well, then use paper towel to turn off water

Answer 3

Disinfect Lab Bench:

1. Spray disinfectant (sanisol) on lab bench
2. Use paper towel to cover surface w/ disinfectant
3. Let air dry

Question 4

Rules

1. No food or drink
2. Keep lab bench clear of unnecessary materials
3. Store books, etc. in cubbies
4. Use culture tube rack to carry test tubes
5. Tell instructor if you have a spill or accident

Answer 4

Labeling:

1. All containers with contents must be labeled (any with liquid)
2. Must include: full name, date, lab day/time, what’s in container, full name of microbe
3. Some labels may need: name of media, temperature, other important info

Question 5

Labeling:

Glassware: used colored tape write with sharpie

Disposable: colored tape or write directly on it with sharpie

Answer 5

Labeling:

Each student must remove unneeded cultures from incubator and place them on waste disposal cart (clean up cart)

Labels must be removed

Question 6

Biohazard Bag:

Use for disposable biohazards

Never for glass

Bag Procedure when full:
Do not overfill bag
Use twist ties
Set bag on floor next to cart
Hang a new bag

Answer 6

Sharps Container:

Used for anything sharp even if it’s contaminated with biohazard waste

Never pick up broken glass with hands always use a dustpan and sweeper

Question 7

Dust Pan Procedure:

If dustpan becomes contaminated with biohazard:

1. spray with disinfectant
2. let sit for 15
3. wash at the sink

Answer 7

Reusable Pipette disposal:

1. Remove pump by grasping white part
2. Place pump in bin
3. Place glass pipette in container with the tip pointed down

Question 8

Reusable glassware contaminated with microbes:

Dispose of:

1. Any culture in a glass test tube
2. Any glass test tube used in an experiment
3. Any culture in a glass petri plate
4. Any use glassed petri plate

All labels must be removed from glassware prior to disposal

Answer 8

Glass Petri dish:

Are placed in metal sleeves

oriented with the cover of the dish on top

Question 9

Glass test tubes:

1. Are placed in metal baskets
2. carefully lay them straight so they don’t spill
3. fill one basket before filling another
4. small test tubes are placed in separate baskets from large test tube’s

Answer 9

Other glassware and reusable containers:

1. Should be washed at the sink
2. Dried
3. Put back in the drawer cabinet that is labeled for such glassware or item

Question 10

Regular trash:

Is located at the ends of the lab benches

Dispose of:
Anything that is not sharp not contaminated or not hazardous

Examples: paper towels or gloves not use to clean up biohazard spill, used labels, paper, uncontaminated packaging

Answer 10

Biohazard spill procedures:

1. Inform instructor and other students
2. Spray thoroughly with disinfectant
3. Use dustpan and sweeper for broken glass and dispose of any sharps
4. Disinfect dustpan and sweeper
5. Cover spell with paper towels
6. Saturate paper towels with disinfectant
7. Leave for at least 15 minutes
8. Change gloves and wash hands
9. After 15 minutes wipe up spell place paper towels in biohazard bag
10. Change gloves and wash hands
11. Re-spray area and wife with paper towels
12. Please paper towels in biohazard bag
13. Change clothes and wash hands

Question 11

Disposal of any Reagent containers:

give this to your instructor for proper disposal

Answer 11

Biohazard bag:

Plastic pipette
plastic petri dish 
oxide strip 
cotton swab 
gloves 
disposable test kit

Question 12

Sharps container:

Anything sharp even if it’s contaminated

Broken glass 
glass test strips 
needles 
razors 
etc.

Answer 12

Glass Petri dish:

Place in metal sleeve

Oriented with the cover of that dish on top

Question 13

Reusable glassware:
Should be disposed of on the cleanup cart

Non-contaminated reusable glassware/containers:
Washed in sink
dried
put back in label drawer/cabinet

Answer 13

Glass test tubes:

Placed in metal baskets
Set straight up so they don’t spill
Fill one basket before filling another
Small test tubes are placed in separate baskets from larger test tubes

Question 14

Glass Petri dish:

Are placed in metal sleeves
oriented with the cover of the dish on top

Answer 14

Reusable Pipette disposal:

1. Remove pump by grasping white part
2. Place pump in bin
3. Place glass pipette in container with the tip pointed down

Question 15

Hand-washing:

Microbial Hot Spots:

1. Fingernails
2. Between fingers
3. Jewelry
4. Wrist

Answer 15

Hand-washing Procedures:

1. Read about hand-washing
2. Have paper towel ready
3. Turn on water
4. Get soap
5. Lather up and rinse while rubbing hands together for 30 seconds
6. Dry hands well, then use paper towel to turn off water