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MT6319 > [LAB] UNIT 4.3 Gel Electrophoresis > Flashcards

[LAB] UNIT 4.3 Gel Electrophoresis Flashcards

(48 cards)

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1
Q

This is the process of separating the charged
constituents of a sample by means of an electrical
current.

A

Electrophoresis

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2
Q

In electrophoresis, separation is done by means of _________ ________

A

electric current

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3
Q

2 main components of electrophoresis

A

Chamber
Power Source

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4
Q

Positively charged pole that attracts negative particles

A

Anode

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5
Q

Color of Anode

A

Red

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6
Q

Negatively charged pole that attracts positive particles

A

Cathode

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7
Q

Color of Cathode

A

Black

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8
Q

Flow of electric current

A

Cathode –> Anode –> Power Source

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9
Q

Electrophoresis is used to separate __________
charged particles.

A

negatively

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10
Q

An ion that contains two functional groups

A

Zwitterion

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11
Q

T or F: A zwitterion is an ion possessing both positive and negative electrical charges

A

T

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12
Q

T or F: A zwitterion is mostly positively charged

A

F

It is mostly neutral

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13
Q

T or F: DNA is negatively charged and will travel towards the cathode

A

F

It will travel towards the anode (positive pole)

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14
Q

Specific buffer needed for electricity to flow

A

Tris-Acetate-EDTA Buffer

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15
Q

Migration of charged macromolecules in the
presence of an electrical power through a porous
support

A

Zone Electrophoresis

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16
Q

T or F: Zone electrophoresis requires electrical power

A

T

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17
Q

Examples of porous support used in zone electrophoresis

A

Paper
Cellulose acetate
Agarose gel

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18
Q

Has a net charge that can be either positive or
negative depending on pH conditions

A

Amphoteric

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19
Q

Movement of buffer ions & solvent relative to the
fixed support

A

Electroendosmosis / Endosmosis

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20
Q

Migration of small charged ions

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A

Iontophoresis

21
Q

5 components of electrophoresis

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A
  1. The driving force
  2. The support medium
  3. The buffer
  4. The sample
  5. The detecting system
22
Q

Examples of general support media

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A

Cellulose acetate
Agarose gel
Polyacrylamide gel

23
Q

Carcinogenic stain used for visualization of analyte

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A

Ethidium bromide (EtBr)

24
Q

In the first step of electrophoresis, EtBr is added to a flask containing ________ _____

25
T or F: Adding EtBr to agarose gel requires shaking or swirling
T
26
Added in order to track the migration of the analyte across the gel
Tracking dye
27
Added by some manufacturers in their tracking dyes
Glycerol
28
T or F: DNA can be seen even in the absence of dyes
F
29
Example of a tracking dye
Cotton Phenol Blue
30
T or F: GelRed is an alternative fluorescent detection dye
T
31
T or F: EtBr is a detection dye
T
32
What equipment is used to place the analyte into the wells?
Pipette
33
This will give proteins the net electric (negative) charge that allows them to move from the negatively charged electrode to the positively charged electrode
Buffer
34
Red wire provides a _______ charged electrode Black wire provides the _______ charged electrode
positively ; negatively
35
A standard piece of equipment used in life science laboratories for visualization of target DNA
UV Transilluminator
36
Unit used in electrophoresis
bp (base pairs)
37
It is a reference to estimate the size of unknown DNA molecules that were separated
DNA Ladder
38
T or F: The closer the sample to the well, the heavier it is. The farther the sample to the well, the lighter it is.
T
39
The best stain to use for DNA extraction is _______ _______ because it allows for intercalation or insertion between layers
ethidium bromide
40
T or F: Gel M is safer and more sensitive than EtBr
F It is less sensitive
41
T or F: DNA Ladder is treated the same as the samples
T
42
T or F: Turn on the power prior to running
T
43
T or F: Results of electrophoresis is merely quantitative
F Results are both quantitative and qualitative
44
Fractions of Separated Proteins
1. Albumin 2. Alpha 1 3. Alpha 2 4. Beta 5. Gamma globulins
45
Stains for visualization of bands
● Amido Black ● Ponceau S ● Oil Red O ● Sudan Black ● Fat Red 7B ● Coomassie Blue ● Gold/Silver Stain
46
The movement of molecules towards the gel is dependent of their
molecular weight
47
T or F: Lower voltages are used for longer periods of time, while higher voltages for shorter periods of time
T
48
T or F: The dye somewhat serves as the standard curve
F The DNA Ladder somewhat serves as the standard curve

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