Laboratory Detection of Bacterial Infections

Question 1

Gold standard method in identifying bacterial infection as well as isolating it

Answer 1

Culture

Question 2

Traditional means of detencting bacterial agents

Answer 2

Bactrial culture

Question 3

What are the 3 culture media

Answer 3

Enriched
Selective
Differential

Question 4

Media that inhibits growth of groups of organisms while facilitating the groups of another

Answer 4

Selective media

Question 5

Allows to differentiate the use of bacteria based on their color characteristics

Answer 5

Differential agar

Question 6

MacConkey agar is a what type of agar?

Answer 6

Selective and differential

Question 7

2 types of stain used in microscopy?

Answer 7

Differential and Fluorescent

Question 8

Example of differential stain?

Answer 8

Gram
Acid fast
Giemsa

Question 9

Example of Fluorescent?

Answer 9

Fluorescein isothioscyanate

Auramine rhodamine

Question 10

Limitation of fluoescent stain?

Answer 10

Training is needed

Not all organisms can be seen in this microscope

Question 11

Microscope that allows to see viral particles

Answer 11

Electron

Question 12

The most widely known method for molecular?

Answer 12

PCR

Question 13

Methhod of hoice for N. gonorrhoeae and C. trachomatis

Answer 13

Nucleic acid-based assay

Question 14

Highly specific and sensitivities have improved dramatically

Answer 14

Antigen detection

Question 15

Elisa

Latex agglutination

Lateral flow immunochromatography

Is an example of?

Answer 15

Antigen detection

Question 16

Detect and confirm infection from organisms that are difficult to grow

Useful in determining the causative agent when clinical manifestation are not specific enough

Disadvantage:
Delay between start of infection and antibody production

Answer 16

Antibody detection

Question 17

Major cause of bacterial pahryngitis and childhood impetigo

Answer 17

Group A strep (S. pyogenes)

Question 18

Primary virulence factor for S. pyogenes?

Answer 18

M protein

Question 19

Filamentous molecule consisting of 2 alpha-helical chains that extend out from the cell surface

Answer 19

M protein

Question 20

Gene codes for M protein

Answer 20

emm genes

Question 21

Virulence factor that:

Limits the depositio of C3 on the bacterial surface

Answer 21

M protein

Question 22

Virulence factor that:

Causes scarlet fever

Answer 22

Pyogenic exotoxins

Question 23

Strep throat and impetigo are the major sites of infection

T or F

Answer 23

T

Question 24

Due to infection with GAS that produces pyrogenic exotoxins

Answer 24

Scarlet fever

Question 25

Antibodies of diagnostic significance for GAS?

Answer 25

ASO Anti-DNase

Question 26

Detects antibodies to the streptolysin O enzyme produced by GAS

Answer 26

Asntistreptolysin testing

Question 27

Clinically useful for patients suspected of having PSGN preceeded by streptococcal skin infections

Answer 27

Anti-DNase B testing

Question 28

A slide agglutination screening test for the detection of antibodies against streptococcal antigens

Answer 28

Streptozyme testing

Question 29

is oxygen labiled hemolysin produced by S. pyogenes

Answer 29

Streptolysin O

Question 30

Strepthroat usually negative in ASO? T or F

Answer 30

T

Question 31

Gram-negative, microaerophilic, helical rods

Answer 31

Helicobacter pylori

Question 32

Major cause of gastric and duodenal ulcer

Answer 32

Helicobacter pylori

Question 33

MOT or H. pylori?

Answer 33

Fecal-oral route

Question 34

Major virulence factor for H. pylori

Answer 34

Protein CagA

Question 35

Detection of H. pylori Ag before & after antimicrobial therapy in fecal samples help determine if bacteria has been eliminated following treatment

Answer 35

Fecal Ag test

Question 36

Smallest free living organisms that can grow on artificial media with no cell wall

Answer 36

Mycoplasma pneumoniae

Question 37

MOT for M. pneumoniae

Answer 37

Droplets

Question 38

Inc pd for M. pneumoniae

Answer 38

1-3 weeks

Question 39

Dermatological manifestation of mycoplasma pneumoniae?

Answer 39

Steven-Johnson syndrome Reynaud Syndrome

Question 40

M. pneumoniae is a fasidious microorganisms T or F?

Answer 40

T

Question 41

Appearane in culture for H. pylori?

Answer 41

Fried egg/hockey puck

Question 42

Gram (-) obligate intracellular organism

Answer 42

Rickettsia

Question 43

MOT for Rickettsia?

Answer 43

Tick borne

Question 44

Rickettsia is Can grow in MacConkey? T or F

Answer 44

F Even though it is Gram neg, it cannot survive in artificial media

Question 45

Term when infant ticks up to adulthood, meron syang rickettsia

Answer 45

Transstadial

Question 46

Term whenl parang vertical transmission ng Rickettsia pero for ticks (mother tick to child tick)

Answer 46

Transovarial

Question 47

causative agent for Rocky Mountain Spotted Fever?

Answer 47

Rickettsia rickettsii

Question 48

Gold standard for Rickettssia?

Answer 48

Indirect IFA

Question 49

Ox-19 - (+) Ox-2 - (+) Ox-K - (-)

Answer 49

prowazeki typhi rickettsii

Question 50

Ox-19 - (-) Ox-2 - (-) Ox-K - (-)

Answer 50

akari Coxiella burnetti

Question 51

Ox-19 - (-) Ox-2 - (-) Ox-K - (+)

Answer 51

Orientia tsutsugamushi