Laboratory Equipment

Question 1

What is the function of the Microscope?

Answer 1

Enable vision of elements that are too small to be seen with the naked eye

Question 2

Microscopy

Answer 2

Inspection with magnification

Question 3

Macroscopy

Answer 3

Gross inspection with the naked eye

Question 4

What objects can be identified with microscopy? - CELLS

Answer 4

CELLS Red Blood Cells White Blood Cells Sperm Platelets Looking at ratio and identification

Question 5

What objects can be identidied with microscopy? - URINE

Answer 5

URINE Bacteria Casts Crystals

Question 6

What objects can be identified with microscopy? PARASITES

Answer 6

PARASITES Worm eggs Lice Mites

Question 7

Microscope elements Eye Piece

Answer 7

MONOCULAR BINOCULAR TRINOCULAR Eye piece holds the occular lense. Magnification of x10

Question 8

Microscope elements Nose Piece

Answer 8

Lower end of the body tube Holds the objective lenses Rotate clockwise - LOWER - HIGHER

Question 9

Microscope elements Objective Lenses

Answer 9

Housed in the nose pieces x4 - scanning x10 - low power x40 - high dry x100 - oil immersion

Question 10

Total magnification

Answer 10

Objective lens x Eye piece e.g. 40 x 10 = x400

Question 11

Microscope elements Limb / Arm

Answer 11

Connects the base with the body Supports the stage and condenser

Question 12

Microscope elements Stage

Answer 12

A flat platform oh which the slide is placed. A hole in the centre allows light from the condenser to illuminate the specimen Stage can be moved up and down by the course and fine adjustment knobs.

Question 13

Microscope elements

Mechanical stage

Answer 13

Attached to the stage, holds the slide in place as well as allowing movement of the slide up and down and side to side by using the mechanical stage hand wheel.

Question 14

Microscope elements

Vernier scales

Answer 14

Scales that allows the relocation of a certain point of the slide.

Question 15

Microscope element

Substage condenser

Answer 15

Below the stage Consists of two lenses that condense the light from the light source onto the specimen to make it brighter and the image sharper.

Question 16

Microscope elements

Iris diaphragm

Answer 16

adjusting the aperture of the iris diaphragm with the control can regulate the amount of the light that passes through the condenser

Question 17

Microscope elements

Base

Answer 17

Houses the light source houses on/off switch

Question 18

Microscope elements

Rheostat

Answer 18

changes the intensity of the light

Question 19

Battlement technique

Answer 19

the technique of looking at the body of a slide thoroughly. Start left-hand side

Question 20

Define - Supernatant

Answer 20

Fluid from a spun sample

Question 21

Define - Sediment

Answer 21

Solid from a spun sample

Question 22

Functions of a centrifuge

Answer 22

Seperates sample Serum - cells from fluid PCV - cells of different densities Urine - concentrating material (sediment)

Question 23

Spinning speeds Blood

Answer 23

10,000 RPM for 5 mins

Question 24

Spinning speeds Urine

Answer 24

2,000 RPM for 5 mins

Question 25

Refractometers

Answer 25

Refractometers measure the degree to which the light refracts in the device. The concentration of the liquid changes how much refraction occurs. Should be calibrated before every use

Question 26

Dry chemistry blood analysers

Answer 26

Slide impregnated with chemicals, sample dropped onto slide Read + Interpret colour change

Question 27

Wetchemistry blood analysers

Answer 27

Small containers that have 'wet' chemicals in. Uses chemical reaction to determin level in the sample

Question 28

Spectrophotometer

Answer 28

mechanically measures colour change within a sample colour change represents the levels within a sample

Question 29

Quality assurance

Answer 29

overall programme which ensures final results reported by the laboratory are all correct

Question 30

Quality control

Answer 30

the measures in place to ensure the test is working correctly to ensure accuracy, precision and reliability. Manufacturers will provide a sample with known values to asses any inconsistensies

Question 31

Glassware

Answer 31

Petri Dishes Test tubes conicle flasks slides/coverslips PVC tubes - haemocrit tubes