Laboratory ID of parasites

Question 1

Why the recommendation of multiple stool specimen and ova and parasite examinations

Answer 1

these multiple examinations help to maximize the detection of intestinal ova and parasites, at least 3 independently collected stool specimens

Question 2

how does one examine stool specimens and liquid specimen in particular

Answer 2

exam fresh specimens ( can view motile trophozoites) and preserved specimens. Liquid specimens have to be viewed within 30 mins of passage.

Question 3

how does one examine soft specimen

Answer 3

soft specimen may contain both trophozoite and cyst, examine within 1 hour of passage.

Question 4

how long can we keep formed specimens

Answer 4

up to one day prior to examination, are less likely to contain trophozoites.

Question 5

which preservatives tend to be used if stool is not examined within the allotted time

Answer 5

10% formalin and PVA (polyvinyl alcohol)

Question 6

list two concentration procedures and their usefulness

Answer 6

flotation technique enables for organisms to rise to the top and debris to sink to the bottom and sedimentation technique enables for organisms to fall to the bottom.

Question 7

commercial fecal concentration systems

Answer 7

parasites allowed to pass through the strainer, excess fecal debris retained

Question 8

list the stains used to identify trophozoites and to make out nuclear and cellular parts

Answer 8

Trichome stain ( rapid well stained smears of various composition), Modified Acid fast stain ( smears of oocytes of the coccidian spps ), Chromotrope stain (used to differentiate microsporidia spores from background fecal matter)