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Clinical Bacteriology (Finals) > (Laboratory) Identification of Streptococcus and Enterococcus > Flashcards

(Laboratory) Identification of Streptococcus and Enterococcus Flashcards

1
Q

List down the step-by-step procedure in the Catalase Test of Streptococcus/Enterococcus:

A
  1. Place 1 drop of hydrogen peroxide reagent in a glass slide
  2. Emulsify a colony from the plate using applicator stick
  3. Observe
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2
Q

List down the step-by-step procedure in the Gram Staining of the Streptococcus/Enterococcus:

A
  1. Using a sterile loop, place a loopful of saline into a labelled slide
  2. Emulsify a colony from Plate into the saline
  3. Air dry and heat fix
  4. Stain with gram stain
  5. View under microscope
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3
Q

List down the step-by-step procedure in the Bacitracin disc test (Taxo A) of Streptococcus/Enterococcus:

A
  1. Make a bacterial suspension (pure inoculum) by placing 3-5 colonies from plate into sterile distilled water. Match the turbidity with the MacFarland standard
  2. Using lawn culture method, streak the pure inoculum into a blood agar plate
  3. After drying for 10 minutes, place the 0.04 units bacitracin disc into the blood agar plate using sterile forceps
  4. Incubate
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4
Q

List down the step-by-step procedure in the CAMP Test for Streptococcus/Enterococcus:

A
  1. Inoculate beta-toxin producing S. aureus straight on the center of BAP
  2. Using bacteria from plate, inoculate a straight line at right angle to Staphylococcus streak. Make sure the two streak lines do not touch.
  3. Incubate at 35 C for 18 hours in a candle jar
  4. Observe
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5
Q

List down the step-by-step procedure in the Colonial Morphology of Streptococcus/Enterococcus:

A
  1. Using multiple interrupted streak, inoculate a BAP with bacteria from plate.
  2. Incubate for 18-24 hours
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6
Q

List down the step-by-step procedure in the Hippurate Hydrolysis:

A
  1. Inoculate bacteria on sodium Hippurate substrate to produce a milky suspension
  2. Incubate at 35 C for 2 hours
  3. Add 0.2 mL ninhydrin reagent and further incubate for 15 minutes
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7
Q

List down the step-by-step procedure in the Bile Esculin Test:

A
  1. Using multiple interrupted streak, inoculate a BEA plate with bacteria from plate.
  2. Incubate for 18-24 hours
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8
Q

List down the step-by-step procedure in the Growth on 6.5% NaCl:

A
  1. Inoculate loopful of bacteria to 6.5% NaCl broth
  2. Incubate at 35 C for 18 hours
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9
Q

Gram Stain Morphology of Streptococcus/Enterococcus:

A

Gram-positive cocci in pairs or chains

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10
Q

What is the Catalase Test Result of Enterobacteriaceae:

A

Catalase Negative
No Effeverscence

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11
Q

A test that differentiates Group A Streptococci from other Streptococci

A

Bacitracin Susceptibility Test (Taxo A)

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12
Q

What is the positive result in Bacitracin Susceptibility Test (Taxo A)?

A

Zone of Inhibition
Group A Strep

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13
Q

What is the negative result in Bacitracin Susceptibility Test (Taxo A)?

A

Negative Zone of Inhibition
Other Beta-hemolytic Streptococci spp.

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14
Q

Test for Group B Streptococci except for Hippurate Test:

A

CAMP Test

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15
Q

Positive Result in CAMP Test:

A

Arrowhead hemolysis [Group B Strep] or bowtie appearance [S. agalactiae]

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16
Q

CAMP means:

A

Christie, Atkins, Munch and Petersen

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17
Q

A diffusible extracellular hemolytic heat stable protein

A

CAMP Factor

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18
Q

Test for Group A and Group D Streptococci

A

PYR Hydrolysis Test

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19
Q

Positive Result in the PYR Hydrolysis Test:

A

Cherry Red/Pink

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20
Q

Substrate of the PYR Hydrolysis Test:

A

L-pyrrolidonyl-beta-napthylamide

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21
Q

Reagent of the PYR Hydrolysis Test:

A

p-dimethyl-aminocinnamaldehyde

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22
Q

Enzyme of PYR Hydrolysis Test:

A

Pyrroglutamylamino peptidase or Pyrrolidomylaryl amidase

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23
Q

The only species of streptococcus that is PYR positive

A

Streptococcus pyogenes

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24
Q

A test used to detect the ability of bacteria to hydrolyze substrate hippurate into glycine and benzoic acid by the action of the hippuricase enzyme present in the bacteria.

A

Hippurate Hydrolysis Test

25
Q

A constitutive enzyme that hydrolyzes the substrate hippurate to produce the amino acid glycine

A

Hippuricase

26
Q

Medium of the Hippurate Test:

A

Broth with Hippurate

27
Q

Reagent Indicator in the Hippurate Test

A

Ninhydrin

28
Q

Substrate of the Hippurate Test

A

Sodium Hippurate

29
Q

What is the positive result of the Hippurate Hydrolysis Test?

A

Purple Color
Streptococcus agalactiae

30
Q

_____ is detected by the oxidation with Ninhydrin which results in the production of a deep purple.

A

Glycine

31
Q

A test that differentiate Group D from Beta-hemolytic

A

Bile Esculin Test

32
Q

Enzyme of the Bile Esculin Test

A

Esculinase

33
Q

Media used for the Bile Esculin Test

A

Bile Esculin Agar

34
Q

What is the color of the Bile Esculin Agar?

A

Slightly Brown in color

35
Q

Substrate used in the Bile Esculin Test

A

Esculin

36
Q

What is the positive result of the Bile Esculin Test?

A

Blackening of Medium
Group D

37
Q

Further test from Bile Esculin Test

A

Salt Tolerance Test (6.5% NaCl)

38
Q

What is the negative result of the Salt Tolerance Test (6.5% NaCl)?

A

Clear/Transparent
Non-enterococci

39
Q

What is the positive result of the Salt Tolerance Test (6.5% NaCl)?

A

Turbid
Enterococci

40
Q

Media used in the Salt Tolerance Test (6.5% NaCl)

A

6.5% NaCl Broth

41
Q

Substrate in the Leucine Aminopeptidase Test

A

Leucine-β-naphthylamide → β-naphthylamine

42
Q

Reagent in Leucine Aminopeptidase Test

A

Paradimethylaminocinnamaldehyde reagent

43
Q

Positive result in the Leucine Aminopeptidase Test

A

Red Color
Viridans Streptococci

44
Q

A susceptibility test in conjunction with bacitracin is used for the presumptive identification of beta hemolytic Streptococci on blood agar.

A

SXT Susceptibility Test

45
Q

What specie/s is resistant to SXT Susceptibility Test?

A

Group A & B Strep

46
Q

What specie/s is susceptible in the SXT Susceptibility Test?

A

Other Strep spp.

47
Q

A test that detect carbohydrate component of the cell wall of Streptococci

A

Serological Test

48
Q

Principle of Serological Tests

A

Antigen (from colonies) - Antibody (from Reagent) Reaction

49
Q

Positive result in the Serological Tests

A

Agglutination/clumping [Group A Strep]

50
Q

Negative result in the Serological Tests

A

No agglutination [S. agalactiae]

50
Q

A rapid latex test system for use in the qualitative detection and identification of the lancefield group of Streptococci.

A

Serological Tests

51
Q

Incubation period of CAMP Test

A

35 C for 18 hours

52
Q

How many units of Bacitracin Disc is placed into plate?

A

0.04 units

53
Q

Incubation period of CAMP Test

A

35 C for 18 hours

54
Q

How many units of Bacitracin Disc is placed into plate?

A

0.04 units

55
Q

Incubation period of Hipurate Hydrolysis Test

A

35 C for 2 hours and then after adding 0.2 mL ninhydrin reagent, further incubate for 15 minutes

56
Q

How many ml of ninhydrin reagent is added into the tube for Hippurate Hydrolysis Test?

A

0.2 mL of ninhydrin reagent

57
Q

Incubation period for the Bile Esculin Test

A

18-24 hours

58
Q

Incubation period for the 6.5% NaCl growth

A

35 C for 18 hours

Clinical Bacteriology (Finals) (4 decks)

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