LABORATORY MATERIALS AND INSTRUMENTATION

Question 1

Used to separate substances of different mass or density

Answer 1

CENTRIFUGE

Question 2

RPM stands for

Answer 2

revolutions per minute

Question 3

RCF stands for

Answer 3

relative centrifugal force

Question 4

formula for RCF

Answer 4

RPM2 x r x 1.12 x 10-5

Question 5

RPM of the centrifuge is calibrated using _____

Answer 5

tachometer

Question 6

Swinging bucket type, the centrifuge tubes are held in a vertical position when not moving but are horizontal when the centrifuge is fully in motion

Answer 6

Horizontal head centrifuge

Question 7

Centrifuge recommended for SST

Answer 7

Horizontal head centrifuge

Question 8

Has a fixed 25-52 degrees angle in which the tubes are held during centrifugation

Answer 8

Angle Head Centrifuge

Question 9

Centrifuge adequate for cell packing

Answer 9

Angle Head Centrifuge

Question 10

Generates the highest speed; centrifuge head is held at a fixed angle but generates tight sediment buttons due to the high speed generated

Answer 10

Ultracentrifuge

Question 11

Centrifuge for lipoprotein separation

Answer 11

Ultracentrifuge

Question 12

Pipets are classified according to:

Answer 12

According to design, drainage, and purpose

Question 13

Holds a particular volume but does not dispense the exact volume

Answer 13

To contain

Question 14

Will dispense the exact volume indicated

Answer 14

To deliver

Question 15

Last drop of the liquid should be expelled into the receiving vessel to get the exact volume

Answer 15

Blow out / Blown Out

Question 16

Allows the contents of the pipet to drain by gravity

Answer 16

Self- draining pipet

Question 17

It is a self-draining pipet but the tip should not be allowed to touch the vessel while the pipet is draining

Answer 17

Mohr Pipet

Question 18

Has a graduation marks to the tip and is generally a blowout pipet

Answer 18

Serologic Pipet

Question 19

It is a pipet with a total holding volume of less than 1 mL

Answer 19

Micropipet

Question 20

Used for biological fluids with viscosity greater than that of water (such as blood)

Answer 20

Ostwald Folin Pipet

Question 21

What type of pipet is Ostwald Folin Pipet

Answer 21

Blow out pipet

Question 22

It has the greatest degree of accuracy and precision

Answer 22

Volumetric Pipet

Question 23

Most commonly used mechanical pipet which relies on piston for suction

Answer 23

Air displacement pipet

Question 24

A mechanical pipet that operates by moving the pistol in the pipet/barrier

Answer 24

Positive displacement pipet

Question 25

Plastic pipet is made of ____

Answer 25

Polypropylene

Question 26

Calibrating media for TD and TC pipet

Answer 26

TD: distilled water TC: mercury

Question 27

Wavelength is ____ proportional to amount of energy

Answer 27

inversely

Question 28

<400 nm

Answer 28

UV

Question 29

>700 nm

Answer 29

infrared

Question 30

Absorbance is ___proportional to the concentration

Answer 30

directly

Question 31

Light source for visible - infrared range

Answer 31

tungsten halongen / iodide lamp

Question 32

MC used light source

Answer 32

tungsten halogen lamp (1200 nm)

Question 33

Light source for ultraviolet range

Answer 33

mercury arc lamp, deuterium discharge lamp, xenon arc lamp

Question 34

Function of entrance slit

Answer 34

Minimizes the unwanted or stray light

Question 35

It isolates specific or individual wavelengths

Answer 35

monochromator

Question 36

Give (4) examples of monochromator

Answer 36

colored glass filters, prisms, interference filters, diffraction grating

Question 37

wedge shaped pieces of glass, quartz or NaCl

Answer 37

Prisms

Question 38

most commonly used due to better resolution than prisms

Answer 38

Diffraction gratings

Question 39

Controls the width of light beam (bandpass)

Answer 39

Exit Slit

Question 40

Holds the sample solution

Answer 40

Sample Cuvette

Question 41

Which is more preferred when it comes to the bottom of cuvette? Round or square

Answer 41

Square

Question 42

Convert the transmitted energy into an equivalent amount of electrical energy

Answer 42

detector

Question 43

Has excellent sensitivity and rapid response

Answer 43

Photomultiplier tube

Question 44

Fluoremetry is a.k.a _____

Answer 44

Molecular luminescence spectrophotometry

Question 45

Principles of fluoremetry

Answer 45

1. It measures the amount of light intensity present over a zero background 2. Determines the amount of light emitted by a molecule after excitation by EM radiation

Question 46

Things to remember about fluorometry:

Answer 46

Things to remember about fluorometry: 1. Uses 2 monochromator 2. Monochromator is set at a right angle 3. Uses a UV light source 4. More sensitive and more specific (500-1000x more sensitive than spectro)

Question 47

referes to presence of other substance that could interfere fluorescence

Answer 47

Quenching Phenomenon

Question 48

Stokes effect refers to the difference between ___. ____, ______.

Answer 48

maximum wavelength, excitational, and emitted fluorescence