Laboratory Safety

Question 1

translation of OSHA

Answer 1

Occupational Safety and Health Administration

Question 2

a process that kills all forms of microbial

life, including bacterial spores

Answer 2

sterilization

Question 3

a process that destroys pathogenic organisms, but not necessarily all microorganisms or spores

Answer 3

disinfection

Question 4

physical methods of sterilization

Answer 4

• Incineration
• Moist heat
• Dry heat
• Filtration
• Ionizing (gamma) radiation

Question 5

the most common method of treating

infectious waste

Answer 5

incineration

870 - 980 degrees C
SAFEST method of sterilization
prions are eliminated

Question 6

used to sterilize biohazardous trash and heat-stable objects

Answer 6

moist heat

15 psi (pounds per square inch)
irreversible denaturation of enzymes and structural proteins
fastest and simplest physical method of
sterilization

Question 7

The most commonly used steam sterilizer in the microbiology laboratory

Answer 7

gravity displacement type

Question 8

the to common sterilization temperatures

Answer 8

121 degrees C (250 degrees F)– 15 minutes – media, liquids and instruments

132 degrees C (270 degrees F) – 30 to 60 minutes – infectious medical waste

Question 9

time and temperature requirement of dry heat method

Answer 9

1.5 to 3 hours

160C to 180C

Question 10

method of choice for antibiotic solutions, toxic
chemicals, radioisotopes, vaccines, and carbohydrates,
which are all heat sensitive

Answer 10

filtration

HEPA filters – air – filter up to microorganisms larger than 0.3 um
cellulose acetate or cellulose nitrate membrane with a vacuum – solutions

Question 11

most common chemical sterilant

Answer 11

ethylene oxide (EtO)

Question 12

used to sterilize HEPA filters in BSCs

Answer 12

formaldehyde vapor

vapor-phase hydrogen peroxide

Question 13

sterilant used for medical equipment such as bronchoscopes

Answer 13

glutaraldehyde

– sporicidal

Question 14

cold sterilization uses these reagents

Answer 14

glutaraldehyde

peracetic acid

Question 15

the physical methods of disinfection

Answer 15

• Boiling at 100°C for 15 minutes — kills vegetative bacteria
• Pasteurizing at 63°C for 30 minutes or 72°C for 15
seconds — kills food pathogens without damaging the nutritional value or flavor
• Using nonionizing radiation such as ultraviolet
(UV) light

Question 16

Disinfectants used on living tissue (skin)

Answer 16

antiseptics

Question 17

translation of NFPA

Answer 17

National Fire Protection Association

Question 18

translation os MSDS

Answer 18

(manufacturers’) Material Safety Data Sheets

Question 19

the standard provided by OSHA that ensures all laboratory personnel have thorough working knowledge regarding the chemicals that they handle at work

Answer 19

“Employee right to know” standard

Question 20

RACE

Answer 20

Rescue
Activate
Contain
Extinguish

Question 21

how often all sockets should be checked for electrical grounding and leakage

Answer 21

at least annually

Question 22

frequently acquired laboratory infections in clinical microbiology

Answer 22

shigellosis
salmonellosis
tuberculosis
brucellosis
hepatits

BHTSS

Question 23

frequently acquired infections in NON-MICROBIOLOGY laboratory workers

Answer 23

HBV, HCV, HDV, HIV

Question 24

laboratory personnel that are responsible for ensuring EXPOSURE CONTROL PLAN

Answer 24

Laboratory director

Laboratory supervisor

Question 25

a method wherein infectious wastes are placed in leak-proof plastic bags for sturdiness

Answer 25

double bagging

Question 26

the standard precautions apply to all bloo and other body fluids except:

Answer 26

sweat

Question 27

a device that encloses a workspace in a way as to protect workers from aerosol exposure to infectous disease agents

Answer 27

biologic safty cabinet (BSC)

Question 28

classifications of biosafety cabinets and their specifications

Answer 28

CLASS I BSC:

• sterilizes air that is EXHAUSTED
• negative pressure
• operated with an open front

CLASS II BSC:
-sterilizes air over the infectious material (INSIDE) and the ir EXHAUSTED
-air flows in sheets – vertical laminar flow BSCs
-CLASS IIA: self-contained; 70% of air is recirculated; MOSTLY USED IN MICROBIOLOGY SECTION IN HOSPITALS
-CLASS IIB: air is discharged outside the building; used for radioisotopes, toxic materials, carcinogens
CLASS III:
-completely enclosed
-negative pressure
-most protection for the worker