LDH

Question 1

systematic name of lactate dehydrogenase

Answer 1

EC 11127

Question 2

LDH CATALYZES lactate to _

Answer 2

pyruvate

Question 3

end product for ldh ?

Answer 3

pyruvate + NADH + H+

Question 4

weight of ldh

Answer 4

128,000

Question 5

two different polypeptide chains

Answer 5

h heart and m muscle

Question 6

LD1 AND LD2 TS

Answer 6

heart, rbc, kidney

Question 7

ld3 TS

Answer 7

LUNGS PANCREAS AND SPLEEN

Question 8

LD4 AND LD5

Answer 8

SKELETAL MUSCLE, LIVER AND INTESTINE

Question 9

PRESENCE OF LDH IN RBC

Answer 9

100-150

Question 10

LDH2 IS MORE THAN LDH 1 MEANS:

Answer 10

NORMAL

Question 11

LDH 1 >LDH 2

Answer 11

MYOCARDIAL INFARCTION

Question 12

HIGHEST LDH INDICATES

Answer 12

HEMOLYTIC DISORDERS AND PERNICIOUS ANEMIA

Question 13

LDH 1 WHICH IS IN THE HEART AND RBC CAN INDICATE:

Answer 13

MYOCARDIAL INFARCTION AND HEMOLYTIC ANEMIA

Question 14

LDH 2 SAME ORGANS AS 1 INDICATES :

Answer 14

MEGALOBLASTIC ANEMIA, AMI, HEMOLYZED SAMPLE

Question 15

LDH 5

Answer 15

SKELETAL MUSCLE INJURY AND HEPATIC DISORDERS

Question 16

LDH 3, LUNG LYMPHOCYTES SPLEEN AND PANCREAS

Answer 16

PULMUNARY EMBOLISM, EXTENSIVE PULMONARY PNEUMONIA, LYMPHOCYTIS , AND ACUTE PANCREATITIS CARCINOMA

Question 17

LDH 6

Answer 17

ALCOHOL DEHYDROGENASE

Question 17

LDH 4 IF SEEN IN LIVER

Answer 17

HEPATIC INJURY OR INFLAMMATION

Question 18

LDH 6 IN ARTERIOSCLEROTIC CV FAILURE

Answer 18

SIGNIFIES GRAVE PROGNOSIS AND IMPENDING DEATH

Question 18

THIS MIGRATES CATHODIC TO LDH 5

Answer 18

LDH 6

Question 19

LDH 6 IS ELEVATED IN :

Answer 19

DRUG HEPATOXICITY AND OBSTRUCTIVE JAUNDICE

Question 20

LDH 6 IS RESPONSIBLE FOR THE METABOLIC CONVERSION OF METHANOL AND ETHYLENE GYCOL TO:

Answer 20

TOXIC COMPOUNDS

Question 21

LD 1 PREFERS _ RX

Answer 21

FORWARD

Question 21

_ IS MORE SPECIFIC THAN _ IN THE ASSAY FOR LDH

Answer 21

LACTATE> PYRUVATE

Question 22

LD 5 PREFRES _ RX

Answer 22

REVERSE

Question 23

_ METHOD ( FORWARD )

Answer 23

WACKER

Question 24

PH OF WACKER :

Answer 24

8.8-9.8

Question 25

IT IS THE MOSTH COMMON METHOD

Answer 25

WACKER BOY !

Question 26

PRODUCT OF WACKER

Answer 26

PYRIVATE +NAD

Question 27

PH OF WROBLEUSKI

Answer 27

7.4-7.8

Question 27

T OR F WACKER IS AFFECTED BY PRODUCT INHIBITION

Answer 27

FALSE

Question 28

HAS REVERSE RX AND 3X FASTER THAN FORWARD RX

Answer 28

WROBLEUSKI LA DUE

Question 29

WROBLEUSKI NEEDS SMALLER VOLUME BUT HAS DISADVANTAGES:

Answer 29

SUSCEPTIBLE TO SUBSTRATE EXHAUSTION AND CAN CAUSE LOSS OF LINEARITY

Question 30

WIDELY USED ASSAY FOR LDH WHERE IN IT USES ISOENZYMES

Answer 30

ELECTROPHORESIS

Question 30

ISOENZYMES CAN BE DETECTED BY EITHER

Answer 30

FLOUROMETRICALLY/ COLORIMETRY

Question 30

PRODUCT OF WROBLEUSKI LADUE

Answer 30

LACTATE+NADH

Question 31

IF SAMPLE CANNOT BE PROCESSED IMMEDIATELY:

Answer 31

STORE AT 25C W/IN 25 HRS

Question 32

LDH _ is the most heat labile =, loss of activty starts at _c

Answer 32

5 AT 4C

Question 33

REFERENCE RANGE FOR LDH

Answer 33

100-225 U/L

Question 34

MOST ANODAL TO LEAST ANODAL IN NORMAL SERUM

Answer 34

LDH 2-1-3-4-5

Question 35

THE MAJOR ISOENZYME OF LDH THAT IS PRESENT IN HEALTHY IND.

Answer 35

LDH 2

Question 36

AMI AND INTRAVSCULAR HEMOLYSIS

Answer 36

LDH 1-2-3-4-5