LE cell

Question 1

Autoimmune disorder in which the immune system attacks its own tissues

Answer 1

Systemic lupus erythematosus

Question 2

Drug that caused moon shape face

Answer 2

Steroid

Question 3

Characteristics of systemic lupus erythematosus

Answer 3

Butterfly rash and moon faced

Question 4

Num. of cell needed to become positive in lupus

Answer 4

10 LE cells

Question 5

Anticoagulant of choice

Answer 5

Heparin

Question 6

Num. of glass bead used

Answer 6

5-10 glass beads

Question 7

Purpose on putting glass bead

Answer 7

To rupture the WBC to make the nuclear material bursted out

Question 8

Mechanical rotator mins

Answer 8

30 mins

Question 9

Incubation time and degree

Answer 9

30 mins in 37C

Question 10

Centrifuge period

Answer 10

10 mins in 200g

Question 11

Specimen of choice

Answer 11

Buffy coat (heparinized blood)

Question 12

Stain used

Answer 12

Wright’s stain

Question 13

Num. dip

Answer 13

5

Question 14

View microscope at

Answer 14

Oil immersion objective
Magnification : 100x
Total magnification: 1000x

Question 15

an antibody found in the serum
especially of patients with systemic lupus
erythematosus

Answer 15

Lupus erythematosus factor

Question 16

neutrophil containing a large
spherical body in its cytoplasm

Answer 16

Lupus erythematosus cell