LEC 2ND PPT BUT STAINS ONLY ETC

Question 1

INCLUSIONS

Much Granules

Answer 1

Mycobacterium tuberculosis

Question 2

INCLUSIONS

Babes Ernst Bodies

Answer 2

Corynebacterium diphtheriae

Question 3

INCLUSIONS

Metachromatic Granules

Answer 3

Corynebacterium diphtheriae

Question 4

INCLUSIONS

Volutin

Answer 4

Corynebacterium diphtheriae

Question 5

INCLUSIONS

Halberstaedter Prowazek Bodies

Answer 5

Chlamydia trachomatis

Question 6

INCLUSIONS

Levinthal Cole Lillie Bodies

Answer 6

Chlamydophila psitaci

Question 7

SPECIAL STAINS

Dyar Stain

Answer 7

Cell Wall

Question 8

SPECIAL STAINS

Albert

Answer 8

Metachromatic Granules

Question 9

SPECIAL STAINS

Neisser

Answer 9

Metachromatic Granules

Question 10

SPECIAL STAINS

Fulton-Schaeffer

Answer 10

Endospores

Question 11

SPECIAL STAINS

Dorner

Answer 11

Endospores

Question 12

SPECIAL STAINS

Gray

Answer 12

Flagella

Question 13

SPECIAL STAINS

Leifson

Answer 13

Flagella

Question 14

SPECIAL STAINS

Feulgen

Answer 14

DNA

Question 15

SPECIAL STAINS

Levaditi’s

Answer 15

Spirochetes

Question 16

HOT OR COLD

Kinyoun Method

Answer 16

Cold

Question 17

HOT OR COLD

Ziehl-Neelsen

Answer 17

Hot

Question 18

ZN or Kinyoun:

Which one is safer?

Answer 18

Kinyoun

Because ZN has open flames (toxic)

Question 19

FLAGELLA

Other term for monotrichous

Answer 19

Polar

Question 20

FLAGELLA

Flagella at both ends

Answer 20

Amphitrichous

Question 21

FLAGELLA

Many tufts in one end

Answer 21

Lophotrichous

Question 22

FLAGELLA

Occur all around the bacterium

Answer 22

Peritrichous

Question 23

PILI OR FIMBRIAE

for DNA exchange

Answer 23

Pili

Question 24

T/F:

Capsules act as virulence factors

Answer 24

True

Question 25

# T/F: Bacteria are eukaryotes

Answer 25

False | prokaryotes

Question 26

# G+ or G- Which one has a periplasmic space?

Answer 26

G-

Question 27

# G+ or G- Which one has a thick murein layer?

Answer 27

G+

Question 28

thick cell wall made up of alternating chains of **mycolic acid** and **trehalose sugars**

Answer 28

Acid Fast Cell Wall

Question 29

# Identify G+ and G- cells that lose their cell walls

Answer 29

L-forms

Question 30

# Identify: Contains most/all of the genetic material

Answer 30

Mesosomes

Question 31

# CAPSULE Polypeptide D-Glutamic Acid Containing | what bacteria?

Answer 31

*Bacillus anthracis*

Question 32

# CAPSULE Hyaluronic Acid Containing | what bacteria?

Answer 32

*Pasteurella multocida*

Question 33

# FLAGELLA Motility is best observed at __ degC

Answer 33

25degC

Question 34

Composition of Endospores

Answer 34

dipicolinic acid calcium complex

Question 35

# INCLUSIONS contains **sulfur** containing granules

Answer 35

Thiobacillus

Question 36

# INCLUSIONS (2) have bipolar bodies

Answer 36

* Yersinia pestis * Pasteurella multocida

Question 37

# T/F: Basic dyes are anionic

Answer 37

False | remember BC+ (basic/cationic +, bind to - charged)

Question 38

# T/F: Acid Dyes are anionic

Answer 38

True | remember BC+ and AN-

Question 39

**Gram staining** is an example of: simple or differential staining?

Answer 39

Differential | 2 or more dyes

Question 40

# CAPSULE Klebsiella pneumoniae is

Answer 40

Polysaccharide Containing

Question 41

# T/F: Only bacteria produce biofilms

Answer 41

False | also fungi

Question 42

# ACID OR BASIC rose bengal

Answer 42

acid stain

Question 43

# ACID OR BASIC safranin

Answer 43

basic

Question 44

most popular negative staining procedure

Answer 44

India Ink

Question 45

__ staining is used to demonstrate the capsule

Answer 45

Indirect/Relief/**Negative**

Question 46

# Enumerate 4 reagents in gram staining

Answer 46

* Primary Stain * Mordant * Decolorizer * Secondary Stain

Question 47

# 4 reagents in gram staining this is for stronger binding

Answer 47

mordant

Question 48

staining method for *Nocardia* and *Mycobacterium tuberculosis*

Answer 48

Acid Fast Staining

Question 49

Primary stain in ZN method

Answer 49

Carbolfuchsin

Question 50

# ZN or KInyoun? Mordant is **Tergitol**

Answer 50

Kinyoun