Lec 3-4 Cloning

Question 1

Steps

Answer 1

1. Generation of DNA fragments (chemical or enzymatic synthesis,
   digestion with restriction enzyme)
2. Incorporation of this fragment into another DNA molecule (vector),
   which contains sequences needed for its replication
3. The vector – with the DNA of interest- is introduced into cells,
   where is replicated
4. Cells containing the DNA of interest are identified or selected

Question 2

Expression of cloned genes

Answer 2

Cloned genes: can be expressed when the
vector contains regulatory sequences for
transcription and translation