LEC 9 PROTEIN SYNTHESIS Flashcards

(37 cards)

1
Q

What’s is protein synthesis?

A

Making protein from DNA via RNA

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2
Q

The production of protein from specific gene is called?

A

Gene expression

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3
Q

What are the two stages of protein synthesis?

A

Transcription & Translation

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4
Q

What happens during ‘transcription’?

A

Specific code from gene create mRNA

DNA&raquo_space; mRNA

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5
Q

What happens during ‘Translation’?

A

Ribosomes reads code from mRNA and make protein (polypeptide chain)

mRNA&raquo_space; Protein

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6
Q

Whats the bases of the FIRST codon?

A

AUG

(Chain always starts with AUG)

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7
Q

What are ‘introns’?

A

Non-coding region of gene - No amino acids

(Non-specific information)

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8
Q

What are ‘Exons’?

A

Coding region of gene

(Specific information)

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9
Q

T/F
Exons are taken by mRNA and introns are removed

A

TRUE

(specific information taken by mRNA - make protein)

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10
Q

Define ‘Genetic code’?

A

List of ‘Amino acid’ & matching ‘mRNA codons’

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11
Q

Which condon is alway the START codon?

A

AUG (Methionine)

Chain always start with

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12
Q

List the three (3) STOP codons?

A

UAA
UAG
UGA

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13
Q

T/F
Stop codons does not encode for amino acid

A

TRUE

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14
Q

Transcription of the gene occurs in the nucleus with an enzyme called?

A

RNA polymerase

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15
Q

RNA copy is called?

A

mRNA

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16
Q

What are the 3 steps to transcription?

A

Initiation, Elongation & Termination

17
Q

Explain initiation? (1st step)

A
  1. Transcription factors binds to > promoter
  2. RNA polymerase binds to > promoter >DNA unwind
18
Q

Explain Elongation? (2nd step)

A

RNA polymerase builds> mRNA transcript

Double helix formed

19
Q

Explain Termination?

A

End of gene > mRNA transcript released

Production stops

20
Q

If information translation goes wrong, what’s the result?

A

Abnormal cells produce > Cancer

21
Q

Is the Statement TRUE or FALSE?

Introns - Remove
Exons - Spliced together

22
Q

Where does Translation occur?

A

Cytosol (cytoplasm)

(mRNA exit nucleus )

23
Q

mRNA information/coding is translated into___?

24
Q

mRNA is translated by____ &____?

A

Ribosome & tRNA

(mRNA only reads ribosomes)

25
What’s the function of ribosomes?
Protein synthesis Site (Reads code from mRNA to make protein)
26
Name the 2 subunits of Ribosomes
Large subunit- tRNA -anticodon Small subunit- mRNA-codon Codon > Anticodon
27
What are the three (3) binding sites of ribosomes?
A, P & E
28
What is bonded to the A site, P site and E site?
A site - Binds incoming tRNA P site - Amino acid added to protein E site - tRNA exit
29
What is Transfer RNA?
Link between mRNA and amino acid > produce protein Note: tRNA make and release amino acid then return to make more
30
What is located at the top and bottom ends of tRNA? 2. What are anticodons?
T- Amino acid B- anticodons 2. tRNA 3 nucleotides - match mRNA codon
31
What are three (3) stages of translation?
Initiation, Elongation and Termination
32
What happens during initiation?
tRNA binds to mRNA
33
What happens during Elongation?
Amino acid linked - peptide bond Chain gets longer (Amino acids joined)
34
What happens during termination?
-Ribosome reaches STOP CODON (UAG, UAA or UGA) -Release factor binds to stop codon - Translation ENDS
35
What is the end product of the translation stages?
Polypeptide chain (Protein chain)
36
Post translational modification
Polypeptides destined: Cytocol < Free ribosome Organelle < Bound ribosome- RER
37
START AND STOP CODONS (MEMORIZE)
Start: AUG Stop: UAA, UAG & UGA