Lec: Megakaryopoiesis and Platelet

Question 1

IL-3, GM-CSF, and M-CSF are required for the conversion of?

Answer 1

Hematopoietic Stem Cell to Common Myeloid Progenitor

Question 2

The factor that stimulates the conversion of megakaryocyte progenitor to erythrocytes

Answer 2

EPO (Erythropoietin)

Question 3

Factors that stimulate the conversion to megakaryocyte erythroid progenitor

Answer 3

IL-3, SCF (stem cell factor), TPO (thrombopoietin)

Question 4

IL-11 and TPO stimulates the conversion of Megakaryocyte Progenitor to?

Answer 4

Platelets

Question 5

The largest hematopoietic stem cell in the bone marrow

Answer 5

Megakaryocyte/ Mature Megakaryocyte

Question 6

This is unique only in the megakaryocytic series

Answer 6

Endoreduplication (endomitosis) (๑>◡<๑)

Question 7

It is a process whereby the nuclear material reduplicates but the nucleus does not divide

Answer 7

Endoreduplication or Endomitosis

Question 8

The three progenitor cells carry the marker?

Answer 8

CD34 (ノ^_^)ノ

Question 9

What are the 3 Progenitor Cells?

Answer 9

1. Burst forming unit (BFU-Meg)
2. Colony forming unit (CFU-Meg)
3. Light density- CFU (LD-CFU- Meg)

Question 10

These carry the marker CD42

Answer 10

MK-I, MK-II, MK-III

Question 11

Hormones/ cytokines responsible for the transformation of MK-I to MK-II (Differentiation to Megakaryocytes)

Answer 11

TPO, L-3, L-11

Question 12

Hormones/ cytokines responsible for the transformation from granualr to mature ( Late maturation)

Answer 12

TPO, L-6_ L-11

Question 13

Hormones/ cytokines responsible for thrombopoiesis

Answer 13

L-6, L-11
(੭ु｡╹▿╹｡)੭ु⁾⁾

Question 14

IDENTIFY:

Cytoplasm is more abundant, less basophilic, and granules begin to form

Answer 14

Promegakaryocyte

Question 15

IDENTIFY:

Cytoplasm contains coarse clumps of granules aggregating into little bundles, which bud off from the periphery to become platelets.

Answer 15

Mature megakaryocyte

Question 16

IDENTIFY:

Its nucleus is round, oval and has multiple nucleoli

Answer 16

Megakaryoblast

Question 17

IDENTIFY:

Multiple nucleoli are visible.
irregular in shape. may show lobulation.

Answer 17

Promegakaryocyte
☝( ◠‿◠ )☝

Question 18

It is the first stage in the Megakaryopoiesis that does not contain nucleolus

Answer 18

Granular Megakaryocyte

Question 19

IDENTIFY:

Nucleus: Multiple nuclei or may show multi-lobulation, Chromatin is coarser. No nucleoli are visible.

Answer 19

Granular Megakaryocyte

Question 20

Occurs as a reserve in case of trauma in the body (ex. hemorrhagic or unexpected bleeding)

Answer 20

Splenic Platelet Pool

Question 21

IDENTIFY:

Nucleus: Multilobulated. No nucleoli visible.

Answer 21

Mature megakaryocyte

Question 22

IDENTIFY:

2-4 um in size, Cytoplasmic fragments of megakaryocyte and it has no nucleus

Answer 22

Platelets (Thrombocytes) ٩(●ᴗ●)۶

Question 23

What is the function of platelets?

Answer 23

Primary Hemostasis and Maintaining Capillary Integrity

Question 24

Platelet normal range:

Answer 24

150,000-450,000/ uL

Question 25

Platelet normal function

Answer 25

>100,000/uL

Question 26

Hemorrhage happens if the platelet count is?

Answer 26

<10,000/uL

Question 27

These are major glycoproteins responsible for Primary Hemostasis

Answer 27

GP Ib and GP IIb/ IIIa Complex ヾ(＠^▽^＠)ﾉ

Question 28

This part of the platelet structure is composed of the membranes and is responsible for platelet Adhesion and Aggregation

Answer 28

Peripheral zone

Question 29

This is composed of the Mitochondria, Alpha granules, dense bodies (delta granules)

Answer 29

Organelle Zone

Question 30

Invaginations of the plasma membrane for release of granule content during platelet activation process.

Answer 30

Surface Connecting system (Open Canalicular system)

Question 31

Composed of protein tubulin that maintains the shape of platelets

Answer 31

Microtubules ┗|・o・|┛

Question 32

Glycoprotein responsible for Platelet Adhesion

Answer 32

GP Ib

Question 33

Glycoprotein responsible for Platelet Aggregation

Answer 33

GP IIb/ IIIa Complex

Question 34

A Cofactor in Fibrin Formation is released from?

Answer 34

Alpha Granules

Question 35

Which Zone has the receptors necessary for platelet Aggregation?

Answer 35

Peripheral Zone

Question 36

Granule formation occurs in?

Answer 36

Promegakaryocyte ୧〳 ＾ ౪ ＾ 〵୨

Question 37

One of the sources of Calcium during Platelet aggregation can be found in?

Answer 37

Dense Tubular system

Question 38

Von Willebrand Factor (vWF) participates in?

Answer 38

Platelet Adhesion

Question 39

Responsible for Platelet shape:

Answer 39

Sol Gel Zone and Structural Zone

Question 40

Provide negative charged phospholipid surface for Factor V and II activation

Answer 40

Platelets

Question 41

Endoplasmic Reticulum that sequesters calcium for Platelet activation

Answer 41

Dense Tubular System

Question 42

PF3 is found in which component of the Platelet?

Answer 42

Peripheral Zone ヾ（*⌒ヮ⌒*）ゞ