Lecture 1 - Neuorpharm basics

Question 1

what glial cell provides physical support and repair, as well as removes excess glutamate which is neurotoxic?

Answer 1

astrocyte

Question 2

oligodendrocytes:
myelinate neurons in the ____.
myelinate one or many axons?

schwann cells:
myelinate neurons in the ____.
myelinate one or many neurons?

Answer 2

CNS;
many;

PNS; one

Question 3

____ are activated in response to neuronal damage. they are clear debris via _____

Answer 3

microglia;

phagocytosis

Question 4

3 structures that form the BBB:

Answer 4

tight junctions between nonfenestrated capillary endothelial cells + basement membrane + astrocyte foot processes

Question 5

BBB:
allows hydro____ molecules in, blocks hydro____ molecules.
glucose and AA cross via _____

Answer 5

phobic, phillic;

carrier mediated transport

Question 6

neurotransmission is triggered by electrical ____ of the neuron aka influx of ____ ions

Answer 6

depolarization;

Na

Question 7

resting potention of neuron is roughly ____;

repolarization is due to ____ _____

Answer 7

-85 mV;

K+ efflux

Question 8

action potentials for a single neuron are of (the same or different) magnitude;

the current carried by a nerve fiber is the result of _____

Answer 8

the same (ie All or None);

summation

Question 9

Ach:
produced where?
\_\_\_\_ receptors are coupled to an ion channel.
\_\_\_\_ receptors are G-protein coupled.
\_\_\_\_ in alzheimers and HD;
\_\_\_ in parkinsons

Answer 9

basal nucleus of meynert;
nicotinic;
muscarinic;
decreased;
increased

Question 10

dopamine:
produced in \_\_\_\_ and \_\_\_\_\_;
D1 receptor is \_\_\_ coupled.
D2 receptor is \_\_\_ coupled. 
\_\_\_\_ in depression, parkinson's;
\_\_\_\_ in schizophrenia, HD.

Answer 10

ventral tegmentum, SN pars compacta;
Gs;
Gi;
decreased;
increased

Question 11

NE:
produced in the \_\_\_\_\_
Alpha 1 receptor is \_\_\_ coupled
Alpha 2 receptor is \_\_\_ coupled;
\_\_\_\_ in anxiety
\_\_\_\_ in depression

Answer 11

locus ceruleus;
Gq;
Gi;
increased;
decreased

Question 12

Serotonin:
produced in the \_\_\_\_;
5HT1 and 2 are \_\_\_\_\_ coupled.
5HT3 is \_\_\_ coupled;
\_\_\_\_ in anxiety,
\_\_\_\_ in depression

Answer 12

raphe nucleus;
GPCR;
ion-channel;
decreased;
decreased

Question 13

GABA:
produced in the ____;
GABA A receptor is ____ coupled.
GABA b and C are ____ coupled

Answer 13

nucleus accumbens;
Cl- channel coupled;
GPCR

Question 14

glutamate:
____ and ___ receptors are ____ coupled;
mGluR is a ____ coupled receptor

Answer 14

AMPA, NMDA, Na channel;

GPCR

Question 15

ion channel vs GPCR:
____ can cause gene expression.
____ is associated with rapid response.
____ is associated with persistent responses

Answer 15

GPCR;
ion channels
GPCR

Question 16

voltage gated ion channels:
activated by \_\_\_\_ charge. 
each ion goes...
Na
Ca
K
Cl

Answer 16

positive;
Na = into cell
Ca = into cell
K = out of cell
Cl = into cell

Question 17

most NT transporters are ____ symporters.

Answer 17

Na

Question 18

saturation assay:
drugs are (radiolabeled or unlabeled);
direct or indirect assesment of affinity of drug for receptor?
direct estimate of receptor ____ aka Bmax;

Answer 18

radiolabeled;
direct (Kd);
density/numbert

Question 19

competition assay:
drug is labeled or unlabeled?
direct or indirect assesment of affinity of drug for receptor?

Answer 19

unlabeled;

indirect (Ki)

Question 20

competitive antagonists:
shift binding curves to the ____;
decrease in ____.
can or cannot be overcome by increasing substrate concentration?

Answer 20

right;
potency;
can

Question 21

noncompetitive antagonist:
shift binding curves ____;
decrease in _____;
can or cannot be overcome by increasing substrate concentration?

Answer 21

down;
efficacy;
cannot

Question 22

partial agonist:
shufts curve ____;
decrease in ____

Answer 22

down;

efficacy