Lecture 10: Principles of Vesicular Traffic Flashcards Preview

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Flashcards in Lecture 10: Principles of Vesicular Traffic Deck (17):
1

Steps of vesicular traffic:

  1. Budding
  2. Uncoating
  3. Transport
  4. Tethering
  5. Docking
  6. Fusion 

2

Budding is initiated by the interaction of a small ____ with the donor membrane 

  • GTP-binding protein
    • ARF for COPI 
    • SAR for COPII

3

4

SAR1 binding ___ coat association 

nucleates

5

SAR1 ____ leads to uncoating (molecular timer) 

GTPase activity

6

Sar1 is activated via ___

Sar1 GEF which removes the bound GDP, allowing GFP to bind

7

COPII can adapt to larger cargo 

A image thumb
8

What are the advantages of transporting along cytoskeletal tracks?

These tracks limit the number of possible destinations and aid in proper targeting 

9

Tethering vs Docking

The primary difference between tethering and docking is the physical distance between the vesicle membrane and the target membrane 

10

The operational definition of docking is the interaction of ____ proteins 

SNARE

11

____ proteins can be used to identify different compartments

SNARE

12

____ proteins facilitate transport vesicle docking 

Rab

A image thumb
13

Activated ____ can organize specialized membrane patches that actively recruit proteins to a fusion site 

Rabs

14

Rabs proteins cooperate as organelles mature 

A image thumb
15

SNARE _____ drives membrane fusion

pairing

16

How do SNAREs drive membrane fusion?

v-SNAREs and t-SNAREs interact to form a stalk, pulling the two membranes closer until the meet and fuse together

17

What are responsible for recycling and unpairing cis-SNARE complexes?

NSF and SNAPs