Lecture 10b and 11

Question 1

1) Where do trypsin and chymotrypsin cut?

Answer 1

Trypsin cuts the C terminal end of Lys and Arg

Chymotrypsin cuts c terminal end of Trp, Phe and Tyr (aromatic groups)

Question 2

2) With respect to delta G, what do enzymes do for the reaction?

Answer 2

• the reaction rate in increased by reducing the activation energy, more products are made so delta G is a negative number

Question 3

3) What are some tests that show the evidence of the ES complex?

Answer 3

Enzyme saturation tests, xray crystallography, change in spectroscopy.

Question 4

4) What exactly does Vo, Vmax, Km, Kcat, 1/Kcat,

Answer 4

Vo is initial rate, Vmax is the highest rate, Km is half of Vmax, Kcat is turnover # (# of substrate molecules converted into product units are 1/S), 1/kcat is time required for one molecule to be processed.