Lecture Flashcards

(47 cards)

1
Q

HOW LONG SHOULD YOU LEAVE SECTIONS IN THE OVEN?

A

30 minutes

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2
Q

THIS IS AN EXAMPLE OF AN ACCENTUATOR.

A

phenol in carbon thionine

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3
Q

True of Mordants

a. insoluble
b. does not participate in the staining reaction
c. phenol in carbon fuchsin
d. does not form a complex

A

Insoluble

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4
Q

THIS IS AN EXAMPLE OF A METACHROMATIC STAIN.

A

toluidine blue

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5
Q

Examine living cells (tissues) that have been removed from an organism.

A

supravital stain

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6
Q

Demonstrate carbohydrates and other substances in the tissue.

A

Periodic Acid Schiff stain

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7
Q

What do you expect to see if you use Langhan’s Iodine stain?

A

Glycogen pink to red

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8
Q

What is the counterstain used in the best carmine method?

A

Ehrlich’s hematoxylin

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9
Q

This is useful for staining encapsulated fungi, e.g. Cryptococcus neoformans.

A

Mucicarmine Stain

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10
Q

What is the color produced if basic fuchsin is added in the mixture for carbohydrate staining?

A

bright magenta

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11
Q

best stained by silver impregnation techniques

A

reticulin fibers

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12
Q

Stain for elastic fibers

a. Verhoeffs
b. Kraijan
c. Weigerts
d. all the choices are correct

A

d. all the choices are correct

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13
Q

a rapid method for staining elastic fibers, fibrin and amyloid, employing congo red

A

kraijan

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14
Q

stain for basement membrane

A

Jones

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15
Q

Method for amyloid demonstration

a. gram’s iodine stain
b. congo red method
c. induced fluorescence staining with thioflavine
d. all the choices are correct

A

d. all the choices are correct

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16
Q

stain for lipids performed on frozen section

A

sudan black

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17
Q

a good stain to identify macrophages

A

non specific esterase

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18
Q

stain for calcium except

a. PTAH
b. Alizarin Red
c. Von Kossa
d. no exception

A

a. PTAH

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19
Q

how does reticulin fibers appear in van gieson’s stain

A

faint pinkish color

20
Q

how does reticulin fibers appear in PAS stain

21
Q

collagen in van gieson’s stain

22
Q

muscle in van gieson’s stain

23
Q

collagen in Masson’s trichrome stain

24
Q

collagen in Gomori’s One Step Trichrome stain

25
Muscle fibers in Mallory's Aniline Blue Stain
red
26
What is an endogenous pigment seen in erythrocytes that is derived from Plasmodium infection?
Hemozoin
27
Which among the following cannot detect iron in tissues? a. Leuco patent blue V stain b. Turnbulls blue stain c. Rhoadanine Stain d. Prussian blue stain
d. Prussian blue stain
28
When stained with Gomori's Methenamine silver stain, monosodium crystals would appear?
black
29
The presence of asbestos bodies in lung tissue is due to deposition of?
silica
30
Apathy's media is used when staining for this substance:
copper
31
Which among the following is seen in Fouchet's solution?
tricholoracetic acid and ferric chloride
32
This is an indirect method of identifying calcium in tissues.
Von-Kossa method
33
What fixative is best for the demonstration of monosodium urates?
95% alcohol
34
Which among the following will not stain myelin? a. Holzer Stain b. Weil's method c. Weiger-Pal d. Luxol Fast Blue
Holzer Stain
35
Which among the following will not stain Nissl bodies? a. methylene blue b. hematoxylin and eosin c. toluidine blue d. thionine e. none of the above
e. none of the above
36
Cajal's Gold sublimate is used to stain?
astrocytes
37
Which stain is not helpful for viewing neuritic plaques in Alzheimer's disease?
Baker's Chromate-Acid Hematin
38
Bone marrow specimen are used for the diagnosis of the following, except a. hemochromatosis b. leukemias c. fever of unknown origin d. metastasis e. none of the choices
e. none of the choices
39
Most commonly employed site for BMA aspirate and biopsy collection.
Posterior
40
A type of smear that gives an excellent detail of cell morphology
spread
41
Simple protein
albumin
42
Acid dyes are attracted to acidic substances.
False
43
A fluorescent staining that gives orange red color for DNA and RNA
Rhodamine
44
During fixation, LIPIDs are retained from tissue during processing
False
45
Adequate solvent for Oil Red O and Sudan Black
70% alcohol
46
When using Sudan Black B stain, nuclei will yield what color?
red
47
NILE Blue Sulfate method, neutral lipids are dissolved by:
Red Oxazine