Lecture 14: Energy Conversion in Mitochondria Flashcards Preview

BIOC 341 > Lecture 14: Energy Conversion in Mitochondria > Flashcards

Flashcards in Lecture 14: Energy Conversion in Mitochondria Deck (48)
Loading flashcards...
1

Why is ATP production called chemiosmotic?

This process is called chemisomotic coupling since chemical bond formation (“chemi”) is linked to membrane transport processes (“osmotic”)

2

2 stages of ATP production?

Stage 1: – High energy electrons are used to set up a proton gradient across the mitochondrial inner membrane Stage 2: – ATP synthase (F-type ATPase) uses the proton gradient to drive ATP production

3

Outer membrane

– Permeable to small molecules

4

Inner membrane

– Cristea – Location of the respiratory chain (electron transport proteins) and ATP synthase

5

Matrix

• Tricarboxylic (TCA) acid cycle, aka Kreb cycle or citric acid cycle • Source of NADH (reduced NAD+, electron for ETS)

6

Food molecules can be broken down into what two things?

-fatty acids -pyruvate

7

Fatty acids and pyruvate are converted to _____ before entering the Krebs (TCA) cycle.

Acetyl-CoA

8

What protein is responsible for dividing mitochondria?

Dynamin

9

The ____ determines the position and timing of mitochondrial fission

ER

10

Mitochondria often align with ___

microtubules

11

Mitochondria are more prevalent in high energy tissues like....

cardiac muscle, sperm tails

12

___ is the primary donor of electrons for the ETS

NADH

13

FADH2 is also utilized as an electron donor, entering at ____

ubiquinone

14

NADH is generated by the catabolism of ___ to CO2 during the TCA cycle

acetyl-CoA

15

Acetyl-CoA is produced from glucose by ___

glycolosis

16

Acetyl-CoA is produced from fatty acids by ___

β-oxidation

17

NADH donates ___ electrons to the ETC

2

18

FADH2 donates ___ electrons to the ETC

4

19

The two components of the electrochemical proton gradient formed by the respiratory chain?

-Proton-motive force due to membrane potential (neg in matrix) -Proton motive force due to PH gradient (basic in matrix)

20

Proton translocating proteins contain...

“proton wires” which are rows of polar or ionic side chains or water molecules spaced at short distances so that proton can jump form one to the other

21

Each subsequent participant in the electron transport chain has an increasingly ___ oxidation reduction (redox) potential

larger

22

NADH readily donates its high energy electrons to ___ bound to proteins within the ETC.

metals

23

Order of proteins in ETC?

24

What are two types of electron carriers?

  • cytochromes
  • Iron-sulfur proteins

25

Cytochromes

– Contain a covalently-bound heme 

– The heme bound iron atom changes from the ferric (Fe3+) to the ferrous (Fe2+) oxidation state whenever it accepts an electron 

26

Iron-sulfur proteins

– Sulfur atoms from cysteine side chains within these proteins bind iron in either a 2S:2Fe or 4S:4Fe configuration 

27

What are the two mobile electron carriers in the ETC?

  • ubiquinone
  • cytochrome C

28

Ubiquinone

• Can carry either one or two electrons 

• Embedded in the membrane via a long chain isoprenoid 

• Helps move proton form matrix to intermembrane space via the Q-cycle 

29

Cytochrome C

• A soluble protein with a bound heme prosthetic group 

• Shuttles electrons from cytochrome c reductase to cytochrome oxidase 

30

What is ubiquinone (Q) converted to as Hydrogens are added to it?

  • 1 H (QH*) = ubisemiquinone
  • 2 H (QH2) = ubiquinol