Why is ATP production called chemiosmotic?
This process is called chemisomotic coupling since chemical bond formation (“chemi”) is linked to membrane transport processes (“osmotic”)
2 stages of ATP production?
Stage 1: – High energy electrons are used to set up a proton gradient across the mitochondrial inner membrane Stage 2: – ATP synthase (F-type ATPase) uses the proton gradient to drive ATP production
– Permeable to small molecules
– Cristea – Location of the respiratory chain (electron transport proteins) and ATP synthase
• Tricarboxylic (TCA) acid cycle, aka Kreb cycle or citric acid cycle • Source of NADH (reduced NAD+, electron for ETS)
Food molecules can be broken down into what two things?
-fatty acids -pyruvate
Fatty acids and pyruvate are converted to _____ before entering the Krebs (TCA) cycle.
What protein is responsible for dividing mitochondria?
The ____ determines the position and timing of mitochondrial fission
Mitochondria often align with ___
Mitochondria are more prevalent in high energy tissues like....
cardiac muscle, sperm tails
___ is the primary donor of electrons for the ETS
FADH2 is also utilized as an electron donor, entering at ____
NADH is generated by the catabolism of ___ to CO2 during the TCA cycle
Acetyl-CoA is produced from glucose by ___
Acetyl-CoA is produced from fatty acids by ___
NADH donates ___ electrons to the ETC
FADH2 donates ___ electrons to the ETC
The two components of the electrochemical proton gradient formed by the respiratory chain?
-Proton-motive force due to membrane potential (neg in matrix) -Proton motive force due to PH gradient (basic in matrix)
Proton translocating proteins contain...
“proton wires” which are rows of polar or ionic side chains or water molecules spaced at short distances so that proton can jump form one to the other
Each subsequent participant in the electron transport chain has an increasingly ___ oxidation reduction (redox) potential
NADH readily donates its high energy electrons to ___ bound to proteins within the ETC.
Order of proteins in ETC?
What are two types of electron carriers?
- Iron-sulfur proteins
– Contain a covalently-bound heme
– The heme bound iron atom changes from the ferric (Fe3+) to the ferrous (Fe2+) oxidation state whenever it accepts an electron
– Sulfur atoms from cysteine side chains within these proteins bind iron in either a 2S:2Fe or 4S:4Fe configuration
What are the two mobile electron carriers in the ETC?
- cytochrome C
• Can carry either one or two electrons
• Embedded in the membrane via a long chain isoprenoid
• Helps move proton form matrix to intermembrane space via the Q-cycle
• A soluble protein with a bound heme prosthetic group
• Shuttles electrons from cytochrome c reductase to cytochrome oxidase
What is ubiquinone (Q) converted to as Hydrogens are added to it?