Lecture 16 (Exam 2) Flashcards
Many microbes establish _______ or persistent infections in which the immune response controls but does not eliminate the microbe and the microbe survives without propagating the infection.
Latent
In many infections, tissue injury and disease may be caused by the host response to the microbe (______ ______) rather than by the microbe itself.
Collateral damage
T/F. Inherited and acquired defects in innate and adaptive immunity are important causes of susceptibility to infections.
True
The lack of _______ immune mechanisms leads to very quick expansion of the pathogen.
Innate
The lack of _______ immunity means the infection is initially controlled by innate immunity, however, the pathogen can not be eliminated completely.
Adaptive
All Abs are produced by _______ cells in primary (bone marrow) and secondary (LNs) lymphoid organs.
Plasma
Abs that mediate protective immunity may be derived from _______ or _______ Ab-producing plasma cells.
Short-lived
Long-lived
Abs perform _______ functions in various tissues distant from their production.
Effector
The effector functions of Abs are triggered ONLY after Ag binding. The effector functions of Abs are mediated by the heavy chain _______ region (Fc) of Ig molecules.
Constant
Different Ab _______ serve distinct effector functions.
Isotypes
The effector functions of Abs against microbes and their toxins are –
1) _______ microbial toxins
2) _______ them for phagocytosis
3) _______ them for Ab-dependent cellular cytotoxicity (ADCC)
4) _______ the complement system
Neutralize
Opsonize
Sensitize
Activate
Isotype switching results in the production of Abs with distinct _____ regions capable of different effector functions.
Fc
Affinity maturation increases the ability of Abs to bind to and ________ or eliminate microbes.
Neutralize
The progressive increase in Ab affinity with repeated stimulation of B cells is one of the reasons for the recommended practice of giving multiple rounds of __________ with the same Ag for generating protective immunity.
Immunizations
T/F. Abs against microbes and microbial toxins block the binding of these microbes and toxins to cellular receptors.
True
Influenze viruses use their envelope protein hemagglutinin to infect respiratory epithelial cells. Gram-negative bacteria use pili to attach to and infect a variety of host cells. Abs bind to these microbial structures and interfere with the ability of the microbes to interact with ________ by means of steric hindrance and thereby may prevent an infection.
Receptors
________ inhibit the spread of microbes from an infected cell to an adjacent uninfected cell.
Antibodies
________ factors refer to the properties of bacterial gene products that enable a microorganism to cause disease.
Virulence
_______ block the binding of toxins to cells and thus inhabit the pathologic effects of the toxins.
Antibodies
Antibodies of the _____ isotype coat (opsonize) microbes and promote their phagocytosis by binding to Fc receptors on phagocytes.
IgG
Binding of _____ receptors on phagocytes to multivalent Ab-coated particles leads to phagocytosis and the activation in phagocytes.
Fc
Isotypes _____ and _____ are the most efficient opsonins for promoting phagocytosis via high-affinity FcyRI (CD64).
IgG3
IgG1
Signals from the _____ receptors activate the phagocytes to destroy these microbes.
Fc
Signals from the _____ receptors activate the phagocytes to destroy these microbes.
Fc
The Fc receptor, FcyRI (CD64) has (HIGH/LOW) affinity for Fc.
High
The Fc receptor, FcyRII (CD32) has (HIGH/LOW) affinity for Fc.
Low
The Fc receptor, FcyRIII (CD16) has (HIGH/LOW) affinity for Fc.
Low
The Fc receptor, FceRI, has (HIGH/LOW) affinity for Fc.
High (binds monomeric IgE)
Ag-Ab complexes can simultaneously bind to the ______ and ______ receptor through the Fc portion of the antibody. As a consequence of this simultaneous ligation of receptors, _________ associated with the cytoplasmic tail of the _______ inhibit signaling by the BCR complex and block B cell activation.
BCR
FcyRIIB
Phosphatases
FcyRIIB
FcR-mediated Ab feedback is a physiologic control mechanism in humoral immune responses. It is triggered by secreted Ab and blocks further Ab production as apposing the activation via ______.
CR2
IgE and _______ are used in the killing of helminths. Worms are too large to be engulfed by phagocytes and they are relatively resistant to the microbicidal products of neutrophils and macrophages.
FceRI
***This binds IgE
IgE, eosinophils, and mast cells function together to mediate the killing and expulsion of some helminthic parasites. Worms can be kill by a toxic cationic protein, known as the _______ _______ _______ present in the granules of eosinophils.
Major basic protein (MBP)
T/F. In Ab-dependent cellular cytotoxicity (ADCC), antibody binds antigens on the surface of target cell. Then, Fc receptors on NK cell recognize bound antibody. Cross-linking of Fc receptors signals the NK cell to kill the target cell. Target cell dies by apoptosis.
True
***See Slide 22
The ________ system consists of serum and cell surface proteins that interact with one another and with other molecules of the immune system in a highly regulated manner to generate products that function to eliminate microbes.
Complement
The complement system is one of the major effector mechanisms of humoral immunity and is also an important effector mechanism of innate immunity. It is activated by microbes and by _____ that are attached to microbes and other antigens.
Abs
Activation of complement involves the sequential ________ of proteins to generate enzyme complexes with proteolytic activity.
Proteolysis
The products of complement activation become ________ attached to microbes, to Abs bound to microbes and to other Ags, and apoptotic bodies.
Covalently
T/F. Complement activation is inhibited by regulatory proteins that are present on microbe and absent in normal host cells.
False. Complement activation is inhibited by regulatory proteins that are present on normal host cells and absent in microbes.
There are three pathways of complement activation, which are…
Classical
Alternative
Lectin
Complement activation depends on the generation of two proteolytic complexes, ______ and ______.
C3 convertase
C5 convertase
This proteolytic complex cleaves C3 into two proteolytic fragments called C3a and C3b.
C3 convertase
This proteolytic complex cleaves C5 into C5a and C5b.
C5 convertase
The alternative pathway is activated by _____ binding to various activating surfaces.
C3b
The classical pathway is activated by _____ binding to Ag-Ab complexes.
C1
The lectin pathway is activated by binding of a ______ ______ to microbes.
Plasma lectin
The late steps of all three complement pathways are (DIFFERENT/THE SAME).
The same
T/F. Complement activated by all three pathways serves the same functions.
True
Complement is initiated by three major pathways. The ________ pathway is spontaneously and continuously activated. The ________ pathway is induced when antibodies bind to their corresponding antigen. The _______ pathway is triggered by the binding of mannan binding lectin (MBL or MBP-C) to mannose residues on the surface of microorganisms.
Alternative
Classical
Lectin
The lectin pathway is triggered by the binding of mannan binding lectin (MBL) to mannose residues on the surface of microorganisms. This activates the _______ and ______, which then cleave ______ and ______.
MASP1 (MBL-associated serine protease 1)
MASP2
C4
C2
Complement activation occurs in a sequential manner. The three pathways initiate the formation of enzymes known as _________.
C3 convertases
The C3 convertase for the alternative pathway is _______, and for the classical and lectin pathways is _______.
C3bBb
C4bC2a
The C3 convertase enzymes cleave the central complement component _____ and generate the anaphylactic and antimicrobial peptide _____ and the opsonin _____, which can be deposited onto any nearby surface.
C3
C3a
C3b
If activation progresses, a new enzyme, the ________, is generated.
C5 convertase
The C5 convertase for the alternative pathway is ________, and for the classical and lectin pathways it is ________.
C3bBbC3b
C4bC2aC3b
C5 convertase cleaves _____, releases the potent anaphylactic peptide ______ and generates _____.
C5
C5a
C5b