Lecture 16: Transcriptional Regulation

Question 1

Fill in the blank: Eukaryotic transcription activators/repressors affect _____ _____ by binding to multisubunit co-activators/co-repressors that modulate chromatin structure or interact with Pol II and general transcription factors

Answer 1

gene expression

Question 2

For the organization of control elements that regulate gene expression in yeast, what do most genes have?

Answer 2

Contain only one upstream activating sequence (UAS) and a TATA box, ~90 bp upstream from the transcription start site.

Question 3

For the organization of control elements that regulate gene expression in eukaryotes, what do most mammalian genes with a TATA box have

Answer 3

• promoter elements
• promoter-proximal elements
• enhancers

Question 4

Explain what promoter elements, promoter-proximal elements and enhancers are

Answer 4

promoter elements:
- Position RNA polymerase II to initiate transcription at the start site.
- Influence the rate of transcription.