Lecture 18: Molecular Bio in Medicine Flashcards
(51 cards)
1
Q
Molecular biology has and will improve
A
human health
2
Q
Molecular cloning is
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3
Q
Molecular cloning purposes
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4
Q
To obtain DNA of interest, one can use?
A
5
Q
Polymerase Chain Reaction (PCR)
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6
Q
PCR amplifies?
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7
Q
Three steps of PCR
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8
Q
Advantages of PCR
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9
Q
Application of PCR
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10
Q
RT-PCR used to obtain?
A
11
Q
PT-PCR enzyme
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12
Q
RT-PCR uses
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13
Q
RT-PCR protocol
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14
Q
RT-PCR can amplify…
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15
Q
Manipulate DNA for cloning uses
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16
Q
Restriction endonucleases (or restriction enzymes) function
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17
Q
Restriction enzymes
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18
Q
“Sticky” ends vs. blunt ends
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19
Q
Palindrome in dsDNA
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20
Q
Restriction enzymes can leave?
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21
Q
After PCR and endonucleases…
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22
Q
Gel electrophoresis
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23
Q
GE, molecules separate based on
Linear DNA separates by….
A
24
Q
DNA staining with fluorescent dyes steps (4)
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25
Polymorphism detection using?
26
RFLPs & example
27
Detection of RFLPs by PCR
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DNA needs to be placed in
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Plasmid/Vector
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Plasmid/Vector uses
31
Plasmid DNA (cloning vector) contains three elements?
32
Molecular cloning
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How to place target DNA into plasmid (2)
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Making recombinant DNA main steps
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B-lactamase
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DNA Ligase in recombinant DNA (3 steps + note)
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DNA sequencing: determine the exact order of nucleotides requires? What method?
37
To confirm the correct clone, what must happen?
38
dNTP
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ddNTP
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Dideoxynucleotide ddNTP function
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DNA polymerase catalyzes?
42
Addition of a nucleotide mediated through 3'-OH group?
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Incorporated dideoxynucleotide lack a?
44
Sequencing reaction mix contains all reagents including?
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Sequencing protocol steps (4)
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Each synthesized DNA has specific length
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DNA length matched to
48
Fluorophore steps
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49
Fluorescent color = ?, Migration in gel = ?
