Lecture 2 Flashcards

Question

BASE

Answer 1

Supports the microscope and houses the lamp and field diaphragm LIGHT SOURCE – Bulb in base – turn on using main switch – adjust amount of light using the Brightness adjustment or Light Intensity Knob When burns out, lamp must be replaced

Answer 2

Horizontal surface that supports the microscope slide for observation Move slide by hand or translational controls Specimen holder/Stage clip(s) holds the slide in place

Answer 3

Graduated locator markings found on the stage Ability to record the exact location of important specimen details or cells on a slide X&Y axis coordinates are noted you can go back to the exact spot with the measurements

Answer 4

Located under stage Can be on right or left (x axis) front back (y axis)

Answer 5

Allow the specimen to be focused moving the stage up and down Coarse adjustment knob – Focuses the image under lower power (generally, larger in size and located on arm of microscope)- DO FIRST Fine adjustment knob – Sharpens the image under all objective powers (generally, smaller in size and located inside coarse adjustment knob)

Answer 6

An opaque structure with a central aperture Located between the light source and the condenser OPEN ALL LIGHT THROUGH CLOSED ALL LIGHT CLOSED

Answer 7

Works in conjunction with the Aperture or Iris Diaphragm Lens system located directly under stage (made up of one or more lenses) Can be raised or lowered, to help focus light passing through and can be centered in the field of view

Answer 8

Located under stage, incorporated with condenser system Controls angle and amount of light

Answer 9

Adjusts condenser lighting focus

Answer 10

Centers image of field diaphragm

Answer 11

method of specimen illumination helps to generate even illumination , reduce artifact and high sample contrast uses diaphragm and condenser

Answer 12

After setting Kohler, when examining a new slide, always bring the specimen into focus using 10x objective first, and then move to higher magnifications especially important when oil immersion rotate the nosepiece so the the objectives dont come in contact with the oil start with the lowest objective with low light intensity and then move on up with higher light intensity

Answer 13

Transport microscope using one hand on the base as a support and one hand firmly around the arm Clean objective lenses and eyepieces with lens paper and lens cleaner or 70% isopropyl (use sparingly) Dry lenses with lese paper NEVER USE: KIMWIPES or PAPER TOWEL - scratches the lenses KLEENEX - leaves lint behind Clean off all residual oil/debris Avoid placing fingers on lens surfaces Set Kohler and document in log Ensure to remove slides from stage when complete turn off microscope when not in use and set back to 10x

Answer 14

check the light source bulb plug plugged in

Answer 15

Oculars - Objective lens (both clean or loose) - Condenser (clean, adjusted, kohler set) - Slide (upside down?) - Coverslip If slides are too thick, rays of light will be focused within the slide and not on the specimen

Answer 16

strains in the neck, back, forearms, wrists, hands and legs/feet may result, which can lead to Musculoskeletal Disorders (MSDs) eyestrain

Answer 17

Interpupillary distance of binocular eyepieces should be adjusted to ensure that both eyes are focusing comfortably Microscope eyepieces should be at an angle of no more than 30°above the horizontal

Answer 18

Neck and Head should bend as little as possible, preferably no more than 10-15º below the horizontal Back should be: Completely upright and flat against the back rest and feet supported equally on the floor or footrest

Answer 19

feet should rest firmly on the floor or a footrest, wrists should be straight or neutral

Answer 20

adequate leg room height-adjustable chair breaks stretch take breaks - 15 mins