Lecture 3 - G-

Question 1

Cause of petechiae?

Answer 1

Endotoxin from G- bacteria

Question 2

Meningitis cause in all ages?

Answer 2

Strep pneumo

N. meningitidis

Question 3

Meningitis cause in 3mos-7yrs?

Answer 3

H. Influenza type B

Question 4

Meningitis cause in neonates?

Answer 4

S. agalactiae
E. coli
Listeria monocytogenes
Strep pneumo+N. meningitidis

Question 5

Meningitis treatment?

Answer 5

Cephalosporins
Fluid/Electrolyte
Corticosteroids

Question 6

N. meningitidis vaccine against what?

Answer 6

capsular polysaccharide

Question 7

N. meningitidis vaccine effective?

Answer 7

Yes, against capsule groups A, C, Y, and W135

Question 8

N. meningitidis vaccine NOT effective?

Answer 8

capsule group B
most common killer today
non-immunogenic

Question 9

N. meningitidis outbreak…give what?

Answer 9

Rifampin

Question 10

Traps molecules >800 MW

Answer 10

Periplasm

Question 11

Protein in PG layer of G-

Answer 11

LPS

Question 12

Endotoxin=

Answer 12

LPS

Question 13

Toxic part of LPS

Answer 13

Lipid A

non-immunogenic

Question 14

Shared among Enterobacteriaceae (heptose + KDO)

Answer 14

Core Polysaccharide

Question 15

Ag specific part of LPS

Answer 15

O somatic Ag

immunogenic

Question 16

LOS

Answer 16

shorter O chain

Same effect as LPS cause has LPS

Question 17

G- Outer membrane structures

Answer 17

Porins

Pili

Question 18

Form of G- bacteria after losing MOST of cell wall

Answer 18

Spheroplasts

can sometimes regen

Question 19

Endotoxin or Exotoxin?

G- only

Answer 19

endo

Question 20

Endotoxin or Exotoxin?

Part of cell wall

Answer 20

endo

Question 21

Endotoxin or Exotoxin?

LPS (not protein)

Answer 21

endo

Question 22

Endotoxin or Exotoxin?

Lethal dose 1 micro g

Answer 22

endo

Question 23

Endotoxin or Exotoxin?

Non enzymatic

Answer 23

endo

Question 24

Endotoxin or Exotoxin?

non-variable genetic traits

Answer 24

endo

Question 25

Endotoxin or Exotoxin? | G+ or G-

Answer 25

exo

Question 26

Endotoxin or Exotoxin? | Secreted

Answer 26

exo

Question 27

Endotoxin or Exotoxin? | Proteins

Answer 27

exo

Question 28

Endotoxin or Exotoxin? | Lethal dose ng or pg

Answer 28

exo

Question 29

Endotoxin or Exotoxin? | Enzymatic

Answer 29

exo

Question 30

Endotoxin or Exotoxin? | Variable genetic traits

Answer 30

exo

Question 31

Endotoxin or Exotoxin? | Enterotoxin

Answer 31

exo

Question 32

bacteria in blood but no symptoms

Answer 32

bacteremia

Question 33

bacteria in blood with symptoms

Answer 33

septicemia

Question 34

Super Ag list

Answer 34

TSST-1 Enterotoxin B/C SPE A/B/C

Question 35

Pyrogenic and cause fever

Answer 35

IL-1beta and IL-6

Question 36

Capillary leak and cause hypotension

Answer 36

TNF-alpha

Question 37

Cause rash and T cell production

Answer 37

IL-2

Question 38

Macrophage activation and rash cause

Answer 38

IFN gamma

Question 39

``` G+ pathway to bacterial induced shock Causative agent: Receptor on Macrophage: IL-1b: TNF-a: IFN-g +IL-2: Lethal dose: ```

Answer 39

``` LTA TLR 2/6 IL 1b TNFa no 1mg ```

Question 40

``` G- pathway to bacterial induced shock Causative agent: Receptor on Macrophage: IL-1b: TNF-a: IFN-g +IL-2: Lethal dose: ```

Answer 40

``` LPS TLR-4 IL 1b TNF a no 1 micro g ```

Question 41

``` Super Ag pathway to bacterial induced shock Causative agent: Receptor on Macrophage: IL-1b: TNF-a: IFN-g +IL-2: Lethal dose: ```

Answer 41

``` Super Ag MHC-II/TCR cross link IL-1b TNF-a IFNg + IL-2 0.1 micro g ```

Question 42

vBeta on TCR has ______ specificity

Answer 42

great