Lecture 3 - Proteins for crystallography

Question 1

Directional and gene-fragmentation cloning can produce many constructs to be

Answer 1

screened for expression

Question 2

Expression system speed (low to high)

Answer 2

Mammalian
Insect
Yeast
Bacteria

Question 3

Expression system cost (low to high)

Answer 3

Bacteria
Yeast
Insect
Mammalian

Question 4

Expression system typical yield (low to high)

Answer 4

Mammalian
Insect
Bacteria
Yeast

Question 5

Expression system post-translational modification (low to high)

Answer 5

Bacteria
Yeast
Insect
Mammalian

Question 6

Expression system FDA approval (low to high)

Answer 6

Insect
Yeast
Bacteria
Mammalian

Question 7

An affinity tag is typically for

Answer 7

purification

Question 8

A fusion partner is typically for

Answer 8

solubility, monitoring expression, or crystallization

Question 9

A cleavage site is for

Answer 9

tag/fusion removal

Question 10

Co-expression of a protein with its binding partner can lead to a

Answer 10

stable complex more suitable for crystallization

Question 11

Antibodies and nanobodies are often used to

Answer 11

1. stabilize proteins
2. serve as crystallization chaperones

Question 12

Lysine methylation can improve crystallization by

Answer 12

changing surface charge properties of the target protein

Question 13

Circular dichroism is used to measure contributions from

Answer 13

alpha-helices, beta-sheets and random coils

Question 14

Light scattering can determine

Answer 14

molecular weight, radius of gyration, diffusion coefficient, and size distributions

Question 15

ThermoFluor stability assay can determine

Answer 15

how stable an array of protein samples are

Question 16

Small angle X-ray scattering can be used to calculate

Answer 16

a molecular envelope of a sample in solution

Question 17

NMR can help differentiate

Answer 17

a folded from an unfolded sample